Search Results (14)

Tissue culture is used to multiply Al-Taif rose (Rosa damascena f. trigintipetala (Dieck) R. Keller) plants in order to meet the demands of the fragrance, cosmetic, and floriculture industries. The use of arbuscular mycorrhizal fungus (AMF) could potentially improve plant growth and acclimatization performance to ex vitro conditions. Thus, in the current study, we investigated how AMF Rhizophagus fasciculatus influences the growth, establishment, and physiological performance of micropropagated Al-Taif rose plants during the acclimatization stage. The growth and physiological parameters of the AMF-treated plants were evaluated after a 12 week growth period in the growth chambers. The plants treated with AMF exhibited greater height (25.53 cm) and biomass growth values for both shoot fresh weight (0.93 g/plant) and dry weight (0.030 g/plant), more leaves (11.3/plant), more leaf area (66.15 cm²), longer main roots (15.05 cm/plant), total root length (172.16 cm/plant), total root area (64.36 cm²/plant), and biomass from both fresh weight (383 mg/plant) and dry weight (80.00 mg/plant) of the plants. The plants treated with AMF also exhibited increased rates of net CO₂ assimilation, stomatal conductance, and transpiration compared to the control plants. The proline content in the leaves and roots was significantly lower in the AMF-treated plants than untreated plants. The Fv/Fm ratio, which serves as an indicator of the intrinsic or maximal efficacy of Photosystem II (PSII) demonstrated a notable decline in the untreated Al-Taif rose plants. These results elucidate the advantageous impact of AMF colonization on micropropagated Al-Taif rose plants, thereby enhancing their resilience against adverse ex vitro conditions. Full article

Micropropagation facilitates the rapid production of roses. Nevertheless, in vitro rhizogenesis of essential oil roses has presented significant challenges, primarily attributed to low rates of rooting and poorer acclimatization compared to ornamental rose varieties. This study reports the optimization of in vitro rooting of Al-Taif rose (Rosa damascena f. trigintipetala (Diek) R. Keller) microshoots with the aim of increasing survival rate during acclimatization. We also investigated the effects of various parameters, including type and concentration of auxin (i.e., 2,4-Dichlorophenoxyacetic acid (2,4-D), indole acetic acid (IAA), indole butyric acid (IBA), and naphthaleneacetic acid (NAA) at concentrations of 0, 0.05, 0.1, 0.2, and 0.4 mg/L), salt strength (i.e., full- and half-strength Murashige and Skoog (MS) medium), sucrose concentration (i.e., 20, 30, 40, 60, and 80 g/L), light spectra (a 2:1 or 1:2 blue/red spectral ratio, cool or warm white light at a 1:1 ratio, and fluorescent light), light intensity (photosynthetic photon flux density (PPFD) values of 25, 50, and 100 µmol·m⁻²·s⁻¹), and activated charcoal (i.e., 0 and 0.5 g/L) on the rooting and growth of in vitro regenerated Al-Taif rose axillary shoots. We found that half-strength MS medium supplemented with 0.2 mg/L NAA, 80 g/L sucrose, 0.5 g/L activated charcoal, and 50 μmol·m⁻²·s⁻¹ PPFD were the optimal conditions for 100% induction of adventitious roots. Next, micropropagated Al-Taif rose plantlets were successfully transferred to a potting medium containing perlite/peatmoss (in a 1:1 ratio). We found that 98% of plants survived ex vitro conditions. The genetic fidelity of micropropagated Al-Taif rose clones along with their mother plant was tested using the inter-simple sequence repeats (ISSR) molecular marker. The genetic similarity between the micropropagated plantlets and the mother plant of Al-Taif rose plants was 98.8%, revealing high uniformity and true-to-type regenerated plants. These findings may therefore contribute toward the commercial micropropagation of Al-Taif roses. Full article

Rose is a widely favored floriculture crop that is commercially propagated through the application of tissue culture techniques. Here, we report an effective method for axillary shoot proliferation in Al-Taif rose, an important cultivar for rose oil industry. Stem nodes were excised from an adult donor Al-Taif rose shrub and cultured for 4 weeks on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP) or gibberellic acid (GA₃) at 0 and 3 mg·L⁻¹ to induce the sprouting of axillary shoots. Al-Taif rose shoots were cultured in vitro for 6 weeks on MS medium fortified with different concentrations of cytokinins, light/dark incubation and different culture types (gelled and liquid/bioreactor culture). The culture conditions that were applied had a noteworthy impact on the responses of Al-Taif rose shoot proliferation. The supplementation of the medium with 6-benzylaminopurine (BAP) resulted in an augmented rate of shoot proliferation in comparison to other cytokinins. Additionally, dark incubation limited foliage growth, leaf yellowing and abscission and favored shoot proliferation compared with light incubation. Liquid culture using bioreactors provided higher axillary shoot proliferation and growth as compared with gelled culture. A continuous immersion system with a net provided the highest axillary shoots (four shoots per explant) and shoot length (16.5 cm), whereas an immersion system without a net provided the highest fresh weight of axillary shoots (499 mg per explant). These findings will improve commercial propagation and contribute to the rose oil industry of Al-Taif rose. Full article

Rose oil production is believed to be dependent on only a few genotypes of the famous rose Rosa damascena. The aim of this study was to develop a novel GC-MS fingerprint based on the need to expand the genetic resources of oil-bearing rose for industrial cultivation in the Taif region (Saudi Arabia). Gas chromatography-mass spectrometry (GC-MS) is a widely used analytical technique for determining the volatile composition of distilled rose oil from flower data. Because biosample availability, prohibitive costs, and ethical concerns limit observations in agricultural research, we aimed to enhance the quality of analysis by combining real observations with samples generated in silico. This study proposes a novel artificial intelligence model based on generative adversarial neural networks (GANs) to classify Taif rose cultivars using raw GC-MS data. We employed a variant of the GAN known as conditional stacked GANs (cSGANs) to predict Taif rose’s oil content and other latent characteristics without the need to conduct laboratory tests. A hierarchical stack of conditional GANs is used in this algorithm to generate images. A cluster model was developed based on the dataset provided, to quantify the diversity that should be implemented in the proposed model. The networks were trained using the cross-entropy and minimax loss functions. The accuracy of the proposed model was assessed by measuring losses as a function of the number of epochs. The results prove the ability of the proposed model to perfectly generate new real samples of different classes based on the GC-MS fingerprint. Full article

The objective of the current study was to examine the chemical composition and biological functions of the various Taif’s rose (TR) organs and floral solid distillation wastes (SDW). Additionally, it assessed the SDW’s potential use in animal feed and potential health applications. For chemical and biological analyses, the plant stems, leaves, and flowers as well as the SDW of TR were gathered from four farms in the Al-Shafa highland region of Taif, Saudi Arabia. The highest levels of cardiac glycosides, flavonoids, and phenolics were found in the flowers (7.66 mg securiaside g⁻¹, 16.33 mg GAE g⁻¹, and 10.90 mg RUE g⁻¹, respectively), while the highest carbohydrate and alkaloid contents were found in the TR leaves (2.09% and 9.43 mg AE g⁻¹, respectively) with no significant differences from the SDW. Quercetin, apigenin, and rutin flavonoids, as well as isocorydine and boldine alkaloids, were found in larger concentrations in the flowers and floral SDW than in the leaves and stems. The various TR flower extracts were effective against Gram-negative and -positive bacteria but had no effect on fungal strains, but the SDW’s methanol extract was only effective against fungi. The plant stem had the highest N, K, and Mg contents (138, 174, and 96.12 mg kg⁻¹, respectively), while the leaves had the highest P and Ca values (6.58 and 173.93 mg kg⁻¹, respectively). The leaves had the highest contents of total carbohydrates and acid detergent fibre (59.85 and 3.93%, respectively), while the stems had the highest total protein and acid detergent fibre (8.66 and 24.17%, respectively), and the SDW had the highest fats and crude fiber (0.57 and 36.52%, respectively). The highest amounts of digestible crude protein, gross energy, and total dissolved nutrients (TDN) (4.52% and 412.61 Mcal kg⁻¹) were found in the plant stem and flowers, respectively. The results of the current experiment showed that the TDN contents of the various organs and the SDW of TR are suitable for mature dry gestating beef cows. It was determined that, in addition to the SDW’s potential usage as an ingredient in animal feed, various plant parts and TR’s SDW can be utilized for a variety of medical reasons. Full article

This study aimed to evaluate the nutrient content and nutritive value of pruning waste of the Taif Damask rose for its potential use as an organic fertilizer or animal forage in the Taif region, Saudi Arabia. For this purpose, the pruning waste of Taif’s rose and soil samples supporting the plant growth at different ages were collected from four farms (F1: 4, F2: 10, F3: 12, and F4: 20 years old). The total aboveground biomass (AGB) of the plants, stems, and leaves were measured in addition to the stem height and crown diameter. The results showed that the maximum stem height and crown diameter (184.20 and 243.5 cm) were recorded in the oldest farm (F4). Moreover, the stem, leaves, and aboveground biomass (AGB) of the waste were maximal (3.91, 1.30 and 5.21 t ha⁻¹) at F4. F1 had the highest N content (154.30 mg kg⁻¹) in the plant leaves, while F2 had the highest stem N and P (172.33 and P 9.40 mg kg⁻¹). Moreover, F3 had the highest concentrations of leaf P (7.17 mg kg⁻¹), leaf and stem K (112.47 and 277.30 mg kg⁻¹), stem Ca²⁺ (251.93 mg kg⁻¹), and leaf and stem Mg²⁺ (122.27 and 123.57 mg kg⁻¹). The stems had higher percentages of total proteins, fibers, ash, and NFE (total carbohydrates) than the leaves in F1 and F2, while the opposite was observed in F3 and F4. The leaves of F2 rose plants had the highest percentage of neutral detergent fibers (NDF), and their stems had the highest percentages of total proteins (10.71%). The leaves of F3 plants had the highest percentage of acid detergent lignin (ADL) and the lowest crude fibers (7.63 and 13.27%), while the stems had the highest NFE (72.71%). The plant–soil relationship expressed by the CCA biplot showed that all the measured plant parameters were at higher positions on the Mg axis, except for the plant height and crown diameter, which were at low positions on the N and NO₃ axes, respectively. In contrast, Cl⁻, NO₃⁻, HCO₃⁻, and SO₄²⁻ had high positive correlations with axis 1 and negative values with axis 2, while EC, the total P, and Ca²⁺ had high positive correlations with, and pH had high negative values in relation to, axis 2. Due to its considerable high inorganic and organic nutrient contents, Taif’s rose could be used in the manufacturing of organic fertilizer. Additionally, the analysis of the nutritive value of the pruning waste supports its use as animal forage. We strongly recommend that further studies be conducted on the application of plant waste as a soil amendment and animal forage in the field. Full article

The current study examines the bioaccumulation potential of Taif rose shrubs by analyzing the shrubs’ wastes. f. At Al-Shafa Highland, four farms with plants of different ages were chosen to collect soil samples and vegetative waste (leaves and stems) for morphological and chemical analysis. The tallest stem and largest crown diameter (184.2 and 243.5 cm, respectively) were found in the oldest (20-year-old) shrubs, which also produced the highest biomass of pruning wastes of stems and leaves (3.9 and 1.3 t/ha, respectively). The 10-year-old shrubs gathered the highest concentration of Co and Pb (1.74 and 7.34 mg kg⁻¹) in the stem and the highest Fe, Mn, and Ni (18.55, 18.60, and 9.05 mg kg⁻¹) in the leaves, while the youngest plants (4 years) accumulated the highest Cr and Zn (0.83 and 13.44 mg kg⁻¹) in their leaves. The highest contents of Cd, Cr, Cu, Fe, Mn, Pb, and Zn were found in the oldest Taif rose stem (34.94, 1.16, 36.29, 49.32, 51.22, 24.76, and 32.51 g ha⁻¹), while the highest contents of Co and Ni were found in the stems of plants that were 10 and 12 years old (3.21 and 9.54 g ha⁻¹, respectively). The Taif rose’s stem and leaves can absorb the majority of heavy metals that have been studied with BAF values greater than one. Significant relationships between various heavy metals in the soil and the same in the stems (Al, Co, and Pb) and leaves (Co, Fe, Mn, Ni, and Pb) of Taif roses have been observed. According to the current findings, the Taif rose is a promising viable and safe crop for heavy metals phytoremediation if it is grown in polluted soil because there is little to no risk of contamination in the use of its end products, high biomass of pruning wastes, and high efficiency of heavy metal removal. Full article

Exposure to cadmium chloride (CdCl₂) causes an imbalance in the oxidant status of the body by triggering the release of reactive oxygen species (ROS). This study investigated the effect of Rosa damascena (R. damascena) extract on oxidative stress, hepatotoxicity, and the injured cardiac tissue of male rats exposed to CdCl₂. Forty male Wistar albino rats were divided into four groups: the vehicle control (1 mg/kg normal saline), the CdCl₂-treated group (5 mg/kg), the R. damascena extract group (100 mg Kg), and the combination of CdCl₂ and R. damascena extract group. Male rats exposed to CdCl₂ showed multiple significant histopathological changes in the liver and heart, including inflammatory cell infiltration and degenerative alterations. Successive exposure to CdCl₂ elevated the levels of hepatic and cardiac reactive oxygen species (ROS), malondialdehyde (MDA), tumour necrosis factor-alpha) (TNF-α) and interleukin -6 (IL-6) and decreased antioxidant defences. The extracts significantly increased the levels of glutathione, superoxide dismutase (SOD), and catalase (CAT), whereas it dramatically decreased the levels of lipid peroxidation (LPO), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and the mRNA of TNF-α and IL-6. R. damascena administration prevented liver and heart injury; suppressed excessive ROS generation, LPO, and inflammatory responses; and enhanced antioxidant defences. In addition, R. damascena upregulated the mRNA of TNF-α and IL-6 in CdCl₂-administered male rats. In conclusion, R. damascena modulated the oxidative stress and inflammation induced by CdCl₂. The hepatic and cardiac tissue damage and histopathological alterations resulting from the CdCl₂-induced oxidative stress were counteracted by the administration of R. damascena extracts. R. damascena enhanced antioxidant defence enzymes in male rats. Full article

This study attempts to utilize newly developed machine learning techniques in order to develop a general prediction algorithm for agricultural soils in Saudi Arabia, specifically in the Taif region. Energy dispersive X-ray fluorescence (EDXRF) measurements were used to develop national predictive models that predict the concentrations of 14 micronutrients in soils of Taif rose farms, for providing high-quality data comparable to conventional methods. Machine learning algorithms used in this study included the simple linear model, the multivariate linear regression (MLR); and two nonlinear models, the random forest (RF) and multivariate adaptive regression splines (MARS). Our study proposes a machine learning (ML) strategy for predicting fertility parameters more accurately in agricultural soils using 10 farms of the Taif rose (Rosa damascena) in Taif, Saudi Arabia as a case study. Results demonstrated that MARS provides higher prediction performance when the number of explanatory variables is small, while RF is superior when the number of variables is large. On the other hand, the MLR is recommended as a moderate method for predicting multivariate variables. The study showed that multivariate models can be used to overwhelm the drawbacks of the EDXRF device, such as high detection limits and an element that cannot be directly measured. Full article

Despite being an important economic component of Taif region and the Kingdom of Saudi Arabia (KSA) as a whole, Taif rose experiences challenges because of uncontrolled conditions. In this study, we developed a phenotyping prediction model using deep learning (DL) that used simple and accurate methods to obtain and analyze data collected from ten rose farms. To maintain broad applicability and minimize computational complexity, our model utilizes a complementary learning approach in which both spatial and temporal instances of each dataset are processed simultaneously using three state-of-the-art deep neural networks: (1) convolutional neural network (CNN) to treat the image, (2) long short-term memory (LSTM) to treat the timeseries and (3) fully connected multilayer perceptions (MLPs)to obtain the phenotypes. As a result, this approach not only consolidates the knowledge gained from processing the same data from different perspectives, but it also leverages on the predictability of the model under incomplete or noisy datasets. An extensive evaluation of the validity of the proposed model has been conducted by comparing its outcomes with comprehensive phenotyping measurements taken from real farms. This evaluation demonstrates the ability of the proposed model to achieve zero mean absolute percentage error ($\mathrm{MAPE}$) and mean square percentage error (MSPE) within a small number of epochs and under different training to testing schemes. Full article

Taif rose (Rosa damascena Mill) is one of the most important economic products of the Taif Governorate, Saudi Arabia. Cadmium chloride (CdCl₂) is a common environmental pollutant that is widely used in industries and essentially induces many toxicities, including hepatotoxicity. In this study, the major compounds in the waste of Taif rose extract (WTR) were identified and chemically and biologically evaluated. GC–MS analysis of WTR indicated the presence of many saturated fatty acids, vitamin E, triterpene, dicarboxylic acid, terpene, linoleic acid, diterpenoid, monoterpenoid, flavonoids, phenylpyrazoles, and calcifediol (vitamin D derivative). The assessment of potential anticancer activity against HepG2 cells proved that WTR had a high cell killing effect with IC50 of 100–150 µg/mL. In addition, WTR successfully induced high cell cycle arrest at G0/G1, S, and G2 phases, significant apoptosis, necrosis, and increased autophagic cell death response in the HepG2 line. For the evaluation of its anti-CdCl₂ toxicity, 32 male rats were allocated to four groups: control, CdCl₂, WTR, and CdCl₂ plus WTR. Hepatic functions and antioxidant biomarkers (SOD, CAT, GRx, GPx, and MDA) were examined. Histological changes and TEM variations in the liver were also investigated to indicate liver status. The results proved that WTR alleviated CdCl₂ hepatotoxicity by improving all hepatic vitality markers. In conclusion, WTR could be used as a preventive and therapeutic natural agent for the inhibition of hepatic diseases and the improvement of redox status. Additional in vitro and in vivo studies are warranted. Full article

This study investigated the phytochemical contents of Taif’s rose pruning wastes and their potential application as phytomedicine, thereby practicing a waste-recycling perspective. In the Al-Shafa highland, four Taif rose farms of various ages were chosen for gathering the pruning wastes (leaves and stems) for phytochemical and pharmacological studies. The leaves and stems included significant amounts of carbohydrates, cardiac glycosides, alkaloids, flavonoids, and other phenolic compounds. The cardiac glycoside and flavonoid contents were higher in Taif rose stems, while the phenolic and alkaloid contents were higher in the plant leaves. Cardiovascular glycosides (2.98–5.69 mg g⁻¹), phenolics (3.14–12.41 mg GAE g⁻¹), flavonoids (5.09–9.33 mg RUE g ⁻¹), and alkaloids (3.22–10.96 mg AE g⁻¹) were among the phytoconstituents found in rose tissues. According to the HPLC analysis of the phenolic compounds, Taif’s rose contains flavonoid components such as luteolin, apigenin, quercetin, rutin, kaempferol, and chrysoeriol; phenolics such as ellagic acid, catechol, resorcinol, gallic acid, and phloroglucinol; alkaloids such as berbamine, jatrorrhizine, palmatine, reticuline, isocorydine, and boldine. Warm water extract was highly effective against Bacillus subtilis, Escherichia coli, and Proteus vulgaris, whereas methanol and cold water extracts were moderately effective against Aspergillus fumigatus and Candida albicans. The study’s findings suggested that Taif’s rose wastes could be used for varied medical purposes. Full article

The flower industry depends on oil and fragrance, which is addressed in the current work. Different concentrations of NaCl (0, 250, 500, 1000, and 1500 ppm) were applied to Taif rose plants (Rosa damascena var. trigintipetala Dieck) to evaluate their effects on growth and essential oil content. Results clearly indicated the highest survival percentage (98.3%) was seen in untreated plants compared to plants under salinity stress. Moreover, increasing the NaCl levels induced an adverse effect on the growth parameters of Taif rose plants, while some essential oil contents were increased to the maximum degree of their tolerance to salinity stress. The extracted essential oils were analyzed using GC/MS. The essential oils of Taif rose plants treated with 500 ppm NaCl recorded the highest values of citronellol, geraniol and phenylethyl alcohol contents (16.56, 8.67 and 9.87%), respectively. NaCl at 250 ppm produced the highest values of heneicosane (13.12%), and then decreased to the lowest value (7.79%) with the increase of NaCl to 1500 NaCl, compared to the control and other NaCl levels. The current results could highlight the impact of salinity stress on Rosa damascena Miller var. trigintipetala Dieck for better economic and industrial applications. Full article

A fragment of 772 bp of the chloroplast maturase K gene was amplified and sequenced for Rosa x damascena trigintipetala variety growing in Taif region of Saudi Arabia. The data were aligned with their counterparts of other varieties already found in the Genbank database and were analyzed by maximum-parsimony, neighbor-joining and maximum-likelihood methods and a single rooted tree was executed. R. x damascena trigintipetala was paraphyletic where one sample [A] clustered with all varieties while the second [B] was basal. R. x damascena was sister to R. x chinensis semperflorens with the later being basal. R. x damascena gori was basal for all taxa studied. R. moschata was inside the clade of R. x damascena. Hybridization could be possible among R. damascena, R. chinensis and R. moschata. The genetic distance and tree topology indicated that [A] variety could be originated from R. moshata while [B] could be originated from gori or R. chinensis semperflorens. We, therefore, may consider that R. x damascena gori or R. chinensis could be the origin of all nowadays R. x damascena varieties. Full article