Search Results (4)

Accumulation of heavy metals (HMs) in agricultural soil following the application of superphosphate fertilisers seems to induce resistance of soil bacteria to HMs and appears to co-select for resistance to antibiotics (Ab). This study aimed to investigate the selection of co-resistance of soil bacteria to HMs and Ab in uncontaminated soil incubated for 6 weeks at 25 °C in laboratory microcosms spiked with ranges of concentrations of cadmium (Cd), zinc (Zn) and mercury (Hg). Co-selection of HM and Ab resistance was assessed using plate culture on media with a range of HM and Ab concentrations, and pollution-induced community tolerance (PICT) assays. Bacterial diversity was profiled via terminal restriction fragment length polymorphism (TRFLP) assay and 16S rDNA sequencing of genomic DNA isolated from selected microcosms. Based on sequence data, the microbial communities exposed to HMs were found to differ significantly compared to control microcosms with no added HM across a range of taxonomic levels. Full article

The toxic effect of exposure to arsenic, As(V), at concentrations of 0 to 30 mg L⁻¹, for 49 days, on epipsammic biofilms, was evaluated in a microcosm experiment. The growth and composition of biofilms developed on sediments containing As concentrations of 31 mg kg⁻¹ and 85 mg kg⁻¹ were compared, using photosynthetic parameters and Live/Dead stains as end points. A toxic effect of arsenic could not be demonstrated; however, biofilm growth was higher over the sediment with higher arsenic concentrations, suggesting the development of pollution-induced community induced tolerance (PICT). Nevertheless, PICT was not observed after exposure to high arsenic concentration in the laboratory, as there were no differences in algal growth between the previous 0 and 30 mg L⁻¹ systems exposed to new 30 mg As L⁻¹ dissolution over 29 days. The algal composition was affected by the added arsenic, and brown algae were the most tolerant compared to green algae and cyanophyceae, as their percentage increased from 25 and 33% in the control samples to 57 and 47% in the samples with the highest added As concentration. In turn, the biofilm development influenced arsenic redistribution and speciation. Arsenic concentration in water decreased with time during the incubation experiment, retained by the sediment particles and the biofilm. In the biofilm, extracellular As was significantly higher (up to 11 times) than intracellular arsenic. As(V) was the predominant species in water and in the biofilm, but products of biotic transformation, namely As(III), DMA(V) and MMA(V), were also found in the solution and in the biofilm in some systems, demonstrating reduction and methylation by the organisms. As a conclusion, a toxic effect was not detected for the concentrations evaluated. Biofilms naturally exposed in the river system to high As concentrations acquire pollution-induced tolerance; however, tolerance was not acquired by exposure to 30 mg L⁻¹ for 29 days in the laboratory. Full article

The assessment of remediation on metal-polluted soils is usually focused on total and/or bioavailable metal content. However, these chemical variables do not provide direct information about reductions in heavy metals pressure on soil microorganisms. We propose the use of bacterial communities to evaluate the efficiency of three remediation techniques: crushed mussel shell (CMS) and pine bark (PB) as soil amendments and EDTA-washing. A soil sample was polluted with different doses of Cu, Ni, and Zn (separately). After 30 days of incubation, the remediation techniques were applied, and bacterial community tolerance to heavy metals determined. If bacterial communities develop tolerance, it is an indicator that the metal is exerting toxicity on them. Soil bacterial communities developed tolerance to Cu, Ni, and Zn in response to metal additions. After remediation, bacterial communities showed decreases in bacterial community tolerance to Cu, Ni, and Zn for all remediation techniques. For Cu and Ni, soil EDTA-washing showed the greatest reduction of bacterial community tolerance to Cu and Ni, respectively, while for Zn the soil amendment with PB was the most effective remediation technique. Thus, bacterial community tolerance to heavy metals successfully detect differences in the effectiveness of the three remediation techniques. Full article

Accumulation of trace elements (including heavy metals) in soil from usage of superphosphate fertilisers induces resistance of soil bacteria to trace elements of environmental concern (TEoEC) and may co-select for resistance to antibiotics (Ab). This study aimed to investigate selection of co-resistance of soil bacteria to Cd, Zn and Hg, and Ab in soils with varied management histories. Genetic diversity of these bacteria and horizontal transfer of Cd resistance genes (cadA and czcA) were also investigated. Soils with either pastoral and arable management histories and either high levels of Cd and Zn, or indigenous bush with background levels of these TEoEC from the Waikato region, New Zealand were sampled. Plate culturing with a range of TEoEC and Ab concentrations, Pollution Induced Community Tolerance (PICT) assay, antibiotic sensitivity, terminal restriction fragment length polymorphism (TRFLP) and horizontal gene transfer (HGT) analyses were employed to investigate co-selection of TEoEC and Ab resistance. Higher levels of bacterial resistance to TEoEC and Ab correlated with higher levels of TEoEC in soil. Bacterial community structures were altered in soils with high TEoEC levels. Cd resistance genes were transferred from donor bacterial isolates, to recipients and the transconjugants also had resistance to Zn and/or Hg and a range of Ab. Full article