Assessment of the Chronic Toxicity and Interactions between Arsenic and Riverbed Biofilms
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling and Characteristics of Water and Sediments
- -
- Eguas (43°13′24.26″ N, 8°45′44.61″ W): not affected by mining activities, so As concentration in sediments was expected to be low.
- -
- Xavarido (43°13′48.82″ N, 8°49′54.29″ W): located 8 km downstream Eguas, just a little downstream from the area affected by mining, and known to have high As concentrations.
2.2. Experimental
2.2.1. Chronic Toxicity Experiment
2.2.2. Natural Tolerance Evaluation
2.3. Analytical Methods
2.3.1. Biofilm Growth and Characteristics
Phytoplankton Pulse-Amplitude-Modulated (Phyto-PAM) Measurements
Live/Dead Analyses
2.3.2. Arsenic
Arsenic in Water
Arsenic in Biofilm
Arsenic in EPS
2.4. Statistics
3. Results
3.1. Effect of As Concentration on Biofilm Growth
3.2. Arsenic in Water and Biofilm
3.3. Development of Tolerance to As
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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pH | Electrical Conductivity (µS cm−1) | Alkalinity (mg L−1) | Total N (mg L−1) | Total P (mg L−1) | |
---|---|---|---|---|---|
Eguas | 7.39 | 151 | 27.1 | 1.64 | 0.045 |
Xavarido | 7.40 | 146 | 26.6 | 1.52 | 0.026 |
Na | K | Mg | Ca | As | |
(mg L−1) | (µg L−1) | ||||
Eguas | 11.4 | 1.6 | 3.6 | 7.0 | 0.85 |
Xavarido | 10.9 | 1.5 | 3.6 | 8.5 | 1.33 |
Particle Size (%) | >2 mm | 2–1 mm | 1–0.5 mm | 0.5–0.2 mm | 0.25–0.1 mm | 0.1–0.05 mm | <0.05 mm |
---|---|---|---|---|---|---|---|
Eguas | 0.64 | 5.11 | 9.26 | 6.28 | 20.00 | 0.11 | 58.62 |
Xavarido | 17.25 | 25.64 | 30.95 | 18.19 | 6.14 | 0.09 | 1.75 |
N% | C% | S% | C/N | Al% | Fe% | Ti% | |
Eguas | 0.60 | 9.19 | 0.13 | 15 | 6.5 | 6.1 | 0.8 |
Xavarido | 0.06 | 0.44 | 0.01 | 7 | 6.3 | 4.7 | 1.4 |
P (mg kg−1) | Mn (mg kg−1) | Ni (mg kg−1) | Zn (mg kg−1) | Cu (mg kg−1) | As (mg kg−1) | ||
Eguas | 1349 | 1126 | 39 | 257 | 36 | 31 | |
Xavarido | n.d. 1 | 994 | 16 | 65 | 11 | 85 |
Day 0 | Day 9 | Day 42 | |||
---|---|---|---|---|---|
Ymax | Eguas | 0 mg L−1 | 0.02 ± 0.02 | 0.59 ± 0.02 | 0.49 ± 0.18 |
0.3 mg L−1 | 0.02 ± 0.02 | 0.57 ± 0.05 | 0.44 ± 0.21 | ||
1 mg L−1 | 0.01 ± 0.02 | 0.61 ± 0.02 | 0.43 ± 0.13 | ||
3 mg L−1 | 0.04 ± 0.04 | 0.59 ± 0.05 | 0.47 ± 0.09 | ||
10 mg L−1 | 0.04 ± 0.04 | 0.59 ± 0.04 | 0.44 ± 0.14 | ||
30 mg L−1 | 0.05 ± 0.05 | 0.50 ± 0.04 | 0.47 ± 0.08 | ||
Xavarido | 0 mg L−1 | 0.12 ± 0.03 | 0.65 ± 0.01 | 0.52 ± 0.05 | |
0.3 mg L−1 | 0.13 ± 0.05 | 0.63 ± 0.02 | 0.54 ± 0.05 | ||
1 mg L−1 | 0.09 ± 0.02 | 0.64 ± 0.01 | 0.49 ± 0.07 | ||
3 mg L−1 | 0.12 ± 0.03 | 0.62 ± 0.02 | 0.50 ± 0.01 | ||
10 mg L−1 | 0.09 ± 0.01 | 0.58 ± 0.02 | 0.53 ± 0.05 | ||
30 mg L−1 | 0.12 ± 0.07 | 0.53 ± 0.04 | 0.44 ± 0.03 | ||
Yeff | Eguas | 0 mg L−1 | 0.17 ± 0.06 | 0.54 ± 0.06 | 0.58 ± 0.01 |
0.3 mg L−1 | 0.04 ± 0.01 | 0.55 ± 0.04 | 0.39 ± 0.18 | ||
1 mg L−1 | 0.10 ± 0.07 | 0.53 ± 0.04 | 0.59 ± 0.01 | ||
3 mg L−1 | 0.06 ± 0.02 | 0.54 ± 0.03 | 0.50 ± 0.05 | ||
10 mg L−1 | - | 0.43 ± 0.03 | 0.51 ± 0.02 | ||
30 mg L−1 | 0.03 ± 0.01 | 0.39 ± 0.05 | 0.55 ± 0.02 | ||
Xavarido | 0 mg L−1 | 0.06 ± 0.06 | 0.48 ± 0.07 | 0.49 ± 0.03 | |
0.3 mg L−1 | 0.12 ± 0.00 | 0.51 ± 0.02 | 0.46 ± 0.08 | ||
1 mg L−1 | 0.08 ± 0.09 | 0.57 ± 0.04 | 0.39 ± 0.08 | ||
3 mg L−1 | 0.03 ± 0.05 | 0.53 ± 0.05 | 0.43 ± 0.11 | ||
10 mg L−1 | 0.04 ± 0.01 | 0.47 ± 0.02 | 0.46 ± 0.03 | ||
30 mg L−1 | 0.16 ± 0.17 | 0.47 ± 0.15 | 0.39 ± 0.01 | ||
qP | Eguas | 0 mg L−1 | 0.62 ± 0.48 | 0.90 ± 0.03 | 0.94 ± 0.01 |
0.3 mg L−1 | 0.32 ± 0.23 | 0.94 ± 0.04 | 0.86 ± 0.12 | ||
1 mg L−1 | −1.31 ± 3.63 | 0.88 ± 0.04 | 0.96 ± 0.05 | ||
3 mg L−1 | 0.07 ± 0.64 | 0.92 ± 0.09 | 0.93 ± 0.03 | ||
10 mg L−1 | −0.74 ± 0.77 | 0.88 ± 0.04 | 0.92 ± 0.04 | ||
30 mg L−1 | −0.05 ± 0.64 | 0.99 ± 0.07 | 0.91 ± 0.06 | ||
Xavarido | 0 mg L−1 | 0.82 ± 0.98 | 0.84 ± 0.07 | 0.85 ± 0.02 | |
0.3 mg L−1 | 6.56 ± 9.56 | 0.86 ± 0.10 | 0.78 ± 0.14 | ||
1 mg L−1 | 0.83 ± 0.61 | 0.89 ± 0.06 | 0.75 ± 0.11 | ||
3 mg L−1 | 3.75 ± 2.17 | 0.87 ± 0.06 | 0.81 ± 0.15 | ||
10 mg L−1 | 0.69 ± 0.27 | 0.80 ± 0.05 | 0.85 ± 0.01 | ||
30 mg L−1 | 3.40 ± 3.38 | 0.91 ± 0.09 | 0.80 ± 0.01 |
Extracellular As | Intracellular As | ||
---|---|---|---|
Eguas | Control | 0.230 ± 0.015 | 0.080 ± 0.007 |
3 mg L−1 | 0.400 ± 0.049 | 0.083 ± 0.033 | |
30 mg L−1 | 6.630 ± 1.669 | 0.325 ± 0.249 | |
Xavarido | Control | 0.182 ± 0.018 | 0.093 ± 0.008 |
3 mg L−1 | 1.571 ± 0.197 | 0.218 ± 0.048 | |
30 mg L−1 | 2.097 ± 0.527 | 0.096 ± 0.031 |
As | Carbohydrates (mg g−1) | As EPS (µg g−1) | As/Carbohydrates | |
---|---|---|---|---|
Eguas | 0.3 mg L−1 | 0.62 ± 0.11 | 0.30 ± 0.06 | 0.5 |
1 mg L−1 | 0.37 ± 0.13 | 0.20 ± 0.10 | 0.5 | |
10 mg L−1 | 0.38 ± 0.28 | 0.34 ± 0.32 | 0.9 | |
Xavarido | 0.3 mg L−1 | 0.24 ± 0.03 | 0.09 ± 0.03 | 0.4 |
1 mg L−1 | 0.60 ± 0.14 | 0.62 ± 0.07 | 1.0 | |
10 mg L−1 | 0.76 ± 0.16 | 1.36 ± 0.09 | 1.8 |
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Barral, M.T.; Rodríguez-Iglesias, D.; Martiñá-Prieto, D.; Paradelo, R. Assessment of the Chronic Toxicity and Interactions between Arsenic and Riverbed Biofilms. Int. J. Environ. Res. Public Health 2022, 19, 12689. https://doi.org/10.3390/ijerph191912689
Barral MT, Rodríguez-Iglesias D, Martiñá-Prieto D, Paradelo R. Assessment of the Chronic Toxicity and Interactions between Arsenic and Riverbed Biofilms. International Journal of Environmental Research and Public Health. 2022; 19(19):12689. https://doi.org/10.3390/ijerph191912689
Chicago/Turabian StyleBarral, María Teresa, Diego Rodríguez-Iglesias, Diego Martiñá-Prieto, and Remigio Paradelo. 2022. "Assessment of the Chronic Toxicity and Interactions between Arsenic and Riverbed Biofilms" International Journal of Environmental Research and Public Health 19, no. 19: 12689. https://doi.org/10.3390/ijerph191912689