Search Results (13)

An analysis of 483 remotely operated vehicle (ROV) dives carried out along the Italian coast on hard substrata at mesophotic depths (40–200 m) allowed an overview of the rich sponge diversity (53 taxa) of the deep continental platform to be obtained for the first time. About 40% of the potential actual species diversity was recognisable using ROV, suggesting that this group is among the richest yet underestimated using this technology in contrast to other megabenthic taxa. Additionally, the study allowed us to gather data on the current basin-scale distribution and bathymetric limits of five common and easily identifiable demosponges with up to 55% occurrence in the explored sites: Aplysina cavernicola, the group Axinella damicornis/verrucosa, Chondrosia reniformis, Foraminospongia spp., and Hexadella racovitzai. Four of these latitudinal distributions were characterised by high occurrence in the Ligurian Sea and a progressive decrease towards the south Tyrrhenian Sea, with an occasional second minor peak of occurrence in the Sicily Channel. In contrast, Foraminospongia spp. showed a maximum occurrence on the offshore reliefs and a second one in the North–central Tyrrhenian Sea, while it was almost absent in the Ligurian Sea. Trophic and biogeographic reasons were discussed as possible causes of the double-peak distributions. The vertical distributions support a more consistent occurrence of all considered taxa in deeper waters than previously known. This suggests that they may more typically belong to the mesophotic realm than the shallow waters, owing to a more extensive sampling effort in the deeper depth range. The five target taxa are typical or associated species of seven reference habitats in the recently revised UNEP/SPA-RAC classification. However, they may create such dense aggregations that they should be listed as new facies in the abovementioned classification. Full article

This study focuses on optimizing time series forecasting models for water demand in a North Italian city as part of the Battle of the Water Demand Forecast (BWDF) challenge. It aims to accurately predict water demands across ten district-metered areas (DMAs) using historical data and weather information over a one-week horizon. The methodology encompasses data preprocessing, including missing data imputation, feature engineering, and novel normalization techniques, followed by the development and hyperparameter optimization of various data-driven models such as random forest, XGB, LSTM, and Prophet. Extensive cross-validation tests assess each model’s performance, revealing that our refined approach markedly enhances forecast accuracy, demonstrating the importance of model and parameter selection for effective water demand forecasting. Full article

Safe water is a global public health concern amid increasing scarcity and pollution. Bottled water production and consumption contribute to these problems. This study examines tap water consumption in Italy, assessing associated sociodemographic factors and related health outcomes such as obesity and self-perceived health status. Data from the Italian National Statistics Institute’s “Aspects of daily life” survey (N = 45,597) were analyzed. Covariates included education, age, gender, economic status, region, concerns about waste and climate change, consumption of carbonated drinks excluding water, alcohol consumption, consumption of vegetables, consumption of snacks, body mass index, and self-perceived health status. Bivariate analyses and mixed-effect logistic regression models explored the associations. People who drink tap water made up 19,674, with a higher prevalence in people aged 45 to 59 old, people with a graduate/post-graduate degree diploma, with optimal economic resources, people concerned about waste production and climate change, and those coming from the north-east regions of Italy. Underweight people showed a higher prevalence of TW consumption as well as those who less than occasionally drank carbonated drinks, drank alcohol, consumed vegetables more than once a day and snacks less than once a week, dairy products more than once a day, sweet less than once a week, cured meat less than once a week, and chicken meat less than once a week, those with no consumption of sheep meat, consumption of beef meat less than once a week and consumption of pork meat less than once a week, and those with a satisfactory level of perceived health status. Regressions showed that all other age classes are less likely to drink tap water than people younger than 20 years old. The category with “inadequate” economic resources is more likely to consume tap water. Low educational classes show a low likelihood of consuming tap water as well as islands. A concern about waste production and climate change is associated with an increased likelihood of consuming tap water. Tap water consumption was negatively associated with obesity but not with a satisfactory self-perceived health status. Insights from this study can inform public health strategies. Full article

Between 2007 and 2022, 112 specimens of newborn and young pelagic sharks were recorded in the waters of Tuscany Region, in the South Ligurian–North Tyrrhenian Seas (north-western Mediterranean basin). The sharks belonged to the Carcharhinus plumbeus (n = 14), Prionace glauca (n = 66), Isurus oxyrinchus (n = 16), Mobula mobular (n = 5) Alopias vulpinus (n = 7) and Hexanchus griseus (n = 4) species. Each animal was correctly identified thanks to the photographs or videos collected. All specimens were incidentally captured with set nets in inshore shallow waters, except bluntnose six-gill sharks, which were bycatch of deep-water bottom-trawl fishery. Body mass, sex, total length and biometric measurements were recorded in 34 baby sharks following the Mediterranean Large Elasmobranches Monitoring (MEDLEM) protocol. The presence of very evident and often non-healed umbilical scar confirmed that some of the sample specimens were newborn. Further confirmation came from the comparison between the total length observed and the size at birth known for the sampled species as reported in the literature. Some baby sharks were preserved in the Museums of Natural History of Pisa and Florence University collections. The importance of the coastal area studied as a possible shark nursery is discussed. Full article

Sea level changes in coastal areas significantly influence port activities (e.g., the safety of navigation). Along Italian coastlines, sea level variations are mainly due to astronomical tides (well known, due to gravitational attraction between Earth, Moon and Sun); however, during the last fifteen years, a high number of “anomalous” tides has been observed: the study of the phenomenon has allowed to attribute its cause to variations in atmospheric pressure (the so-called meteorological tides: sea level drops when atmospheric pressure increases and vice versa); the statistical analysis of acquired data made it possible to evaluate the hydrobarometric transfer factor (a local parameter which represents the correlation between atmospheric pressure changes and consequent sea level variations): it was found that it is usually much larger within gulfs or port basins than offshore areas, where a pressure change of 1 hPa results in a sea level variation of about 1 cm; the statistical analysis described in the following, and aimed at correctly estimating the hydrobarometric transfer factor in harbors, can play a fundamental role in optimizing the management of port waters: its results allow to forecast meteorological tides and therefore future sea level (and depth) variations in a given port basin. The results of the study conducted in the port of La Spezia (North Western Italy) are presented here, together with possible applications on port activities and harbor water management. Full article

Brown trout (Salmo trutta) populations living in Mediterranean peninsulas and the surrounding islands belong to old evolutionary lineages that persisted during Quaternary glaciations. Many of these populations inhabit marginal areas along the south limit of the distribution of the species, where they face hard environmental conditions (drought, high temperatures and sudden shifts in water flow) that can get worse by anthropogenic activities and climate change. In islands, this vulnerable situation is exacerbated by geographical isolation. Sicilian trout remain only in the south-eastern part of the island and, based on their morphological characteristics, they have been recognized as a distinct species named Salmo cetti. Despite their genetic singularity among other Italian trout (a unique Italian native trout of Atlantic origin), the mitochondrial DNA haplotypes that were found in Sicily are clearly related with the brown trout Atlantic lineages from North Africa and the Iberian Peninsula. In the present study, brown trout in four rivers from north-eastern Sicily were genetically analysed. Based on the genotyping of mitochondrial (control region) and nuclear (LDHC, GP1, GP14, GP31, GP34, GP37, GP38, GP42, SS2 and SL) genes, this study aimed to: evaluate the impact of past stocking practices in natural populations; estimate mitochondrial and nuclear gene diversity; and reconstruct the phylogenetic relationships of Sicilian trout. The initial results showed that only trout from the Tellessimo River remain free from hatchery introgression. Gene diversity was low in most populations, and both mitochondrial and nuclear phylogenetic reconstruction related Sicilian trout with old Atlantic lineages. Full article

Slope exposure is known to affect soil biogeochemical processes in mountainous forest ecosystems, but little attention has yet been paid to its influence at a soil aggregate scale. Therefore, we evaluated the effects of slope exposure (north- vs south-facing slope) on the physico-chemical and microbiological properties of bulk soil and dry-sieved and water-stable aggregate size fractions in both organic (OF) and mineral (AE) horizons in an Italian alpine forest. The changes in organic carbon (OC) and nitrogen (ON) fractions were assessed together with a battery of thirteen enzyme activities involved in the main nutrient cycles. In addition, soil biological properties including microbial biomass (estimated as double-stranded DNA content), and microbial activity (assessed as the ratio between the extra-(exDNA) and intracellular (iDNA) fractions of the total soil DNA pool) were determined. The OF horizon at the north-facing slope was enriched in recalcitrant and insoluble OC and ON fractions and characterized by a lower microbial activity, as indicated by the higher exDNA/iDNA ratio with respect to the south-facing slope. On the contrary, exDNA and iDNA contents, microbial biomass, as well as most of the enzyme activities, reached higher levels at the southern exposure in the AE horizon. These exposure-effects were bulk soil- and aggregate size fraction-specific. Overall, lower values of the chemical and microbiological parameters were found in the water-stable fraction. Our findings indicate that slope exposure (and thus topography), soil horizon, and aggregate size distinctly influence soil OC dynamics in mountain ecosystems. Full article

During the last 150 years, the trout-culture industry focused on enhancing trout populations by stocking, in response to the growing anglers’ demand and the habitat degradation associated to the rapid urbanization and hydropower development. The industrialized north of Italy, home to the Italian Alpine and subalpine trout populations, is the source of most of the revenues of the national trout-culture industry. Its rapid growth, and the massive introduction of non-native interfertile trouts eroded the genetic diversity of native lineages, leading to harsh confrontations between scientists, institutions, and sportfishing associations. We review here the state of the art of the taxonomy and distribution of the northern Italian native trouts, presenting both scientific results and historical documentation. We think the only native trouts in this region are Salmo marmoratus, widespread in this region, plus small and fragmented populations of S. ghigii, present only in the South-western Alps. We strongly recommend the interruption of stocking of domesticated interfertile non-native trouts in this area, and recommend the adoption of Evolutionary Significant Units for salmonid fishery management. We further propose future research directions for a sustainable approach to the conservation and ecosystem management of the fishery resources and inland waters of northern Italy. Full article

The quantification of antimicrobial usage (AMU) in food-producing animals can help identify AMU risk factors, thereby enhancing appropriate stewardship policies and strategies for a more rational use. AMU in a sample of 34 farms in the Province of Trento (north-eastern Italy) from 2018 to 2020 was expressed as defined daily doses for animals per population correction unit according to European Surveillance of Veterinary Antimicrobial Consumption guidelines (DDDvet) and according to Italian guidelines (DDDAit). A retrospective analysis was carried out to test the effects of several husbandry practices on AMU. Overall, the average AMU ranged between 6.5 DDDAit in 2018 and 5.2 DDDAit in 2020 (corresponding to 9 and 7 DDDvet, respectively), showing a significant trend of decrement (−21.3%). Usage of the highest priority critically important antimicrobials (HPCIA) was reduced by 83% from 2018 to 2020. Quarantine management, available space, water supply, animals’ cleanliness and somatic cell count had no significant association with AMU. Rather, farms with straw-bedded cubicles had lower AMU levels than those with mattresses and concrete floors (p < 0.05). In conclusion, this study evidenced a decrement in AMU, particularly regarding HPCIA, but only a few risk factors due to farm management. Full article

This project aimed to apply eco-friendly extraction methods to Crocus sativus L. by-product (flowers without stigmas i.e., tepals composed of petals and sepals) to recover extracts with high antioxidant capacity and polyphenol content, to be used in cosmetic products. Flowers grown in two different Italian regions (Sample 1—Alba in Piemonte, north of Italy and Sample 2—Sibillini in Marche, centre of Italy) were subjected for the first time to different eco-friendly microwave-mediated green solvents extractions (MGSE) andquali-quantitative determination in antioxidant molecules. Firstly, the extracts from Sample 1 were selected according to their total phenol content (TPC) by Folin–Ciocalteu’s assay and antioxidant capacity (AC) by spectrophotometric assays. Then, according to preliminary results, MGSE carried out in ethanol 70°, water, and glycerin were selected as the most performing methods and applied to both Samples 1 and 2. The best results were obtained using green solvents, such as water or ethanol 70°, for the samples coming from Marche. The identification and quantification of phenolic compounds, belonging to anthocyanins and flavonols classes, was performed by using UPLC-DAD-ESI-MS. Concerning flavonols content, the most abundant analyte is kaempferol 3-O-sophoroside and the extract in water from Sample 1 showed the higher amount of flavonols, reaching the concentration of 25.35 mg of kaempferol 3-O-glucoside equivalent per gram of tepals DW of raw material. Among anthocyanins, the most abundant was delphinidin 3,5-O-diglucoside and the high concentration of anthocyanin was detected in water and ethanol extract. Two new compounds, myricetin-di-glucoside and primflasine, were identified for the first time in Crocus sativus L. by-product by high-resolution mass spectrometry (HRMS). The green batches obtained by extraction were thus characterized and evaluated for their biological potential and safety in keratinocyte HaCaT cells. The extracts were not cytotoxic up to 0.03 mg/mL. The water and ethanol 70° extracts were the most effective in counteracting oxidative stress induced by H2O2 and UVA exposure and reduced cytotoxicity induced by UVB exposure. The water extract was also able to significantly reduce cytotoxicity induced by sodium dodecyl sulphate-induced damage. Taken together, these results suggest a potential use of these waste materials as cosmeceutical preparations such as antiaging, and as anti-skin irritation formulation by-products. Full article

Saffron is a well-known spice, produced for a long time, mainly in the Mediterranean area. In the last few years, saffron has been seen as an alternative crop for the diversification of agricultural production and a new source of income, due to its high price, including in Alpine areas. Apart from the spice, constituted by the stigmas, saffron production provides a high amount of fresh tepals, which have so far been considered agricultural waste. Recently, studies on the composition of saffron tepals have been revealing their potential as a rich source of bioactive compounds. In this study, we evaluated the productive traits and the quality of the spice of saffron cultivated in open fields in the north western Italian Alps, for 2 years. In addition, in order to valorize the crop residues, we evaluated the bioactive compound content in tepals by using maceration and ultrasound assisted extraction with different rates of water and methanol as solvents. Higher yields in spice were obtained in the second year of cultivation. However, in both years, the spice had a high quality (ISO 3632), and a very high total phenolic content and antioxidant activity. Thirteen bioactive compounds were identified, including flavonols, cinnamic acids, benzoic acids, catechins, and vitamin C, with few differences between sites and year of cultivation. In tepals, the extraction method and the solvent used influenced the evaluated parameters, i.e., total phenolics and total anthocyanins content, antioxidant activity, and the amount of the four phenolic compounds found (hyperoside, rutin, ellagic acid, and epicatechin). Overall, this study revealed that both saffron spice and its by-product, the fresh tepals, produced in the north western Italian Alps can be considered a source of bioactive compounds with nutraceutical properties, having an antioxidant capacity that is often similar or higher than those of some vegetables and fruits. Full article

Hydrogeochemical analyses were carried out to identify geochemical processes occurring in the low-lying coastal aquifer of Ravenna, North Adriatic Sea (Italy). The area is characterized by a complex coexistence of several environments: coastal dunes, paleodunes, pine forests, freshwater wetlands, rivers, brackish lagoons, gravel pit lakes, reclaimed lands, agricultural fields and industrial areas. Water quality is of primary importance for the sustainability of these, areas and a full understanding of geochemical processing is fundamental for their management. A total of 104 water samples was collected from groundwater wells and surface water bodies, and analyzed for the major and trace elements (TEs). Field measurements of chemical-physical parameters were carried out by a multiparameter device XS PCD650; major elements were analyzed following the Italian National Environmental Agency standards (APAT-IRSA 2003), while TEs were analyzed by ICP-AES/ICP-MS. The major findings include: organic matter degradation in salinized and anoxic conditions; TEs concentrations related to water–sediment interactions, i.e., adsorption, ion exchange, redox reactions, mineral dissolution and precipitation; anthropogenic contamination from pesticides and fertilizers use; pollution from industrial district; TEs enrichments and depletion due to groundwater salinization and water management practices; comparison of TEs concentrations with respect to national and international thresholds. The findings can provide water managers and local authorities with a comprehensive framework of the coastal water hydrochemistry, allowing a better understanding of the effects of current management practices and the design of mitigation measures to reduce water resource deterioration in the studied coastal area. Full article

Water in rock masses is a key factor in geo-mechanics, hydrogeology, mining, geo-thermics, and more. It is relevant in interpreting rock mass behavior (e.g., water-rock interaction or slope stability), as well as in defining heat transfer mechanisms. Pointing out the contribution of secondary porosity in increasing advective heat transfer instead of the conduction phenomenon, this study aims to highlight a different thermal response of sound rocks and faulted zones. Moreover, it provides some methodological suggestions to minimize environment disturbance in data collection and a robust interpretation of the results. An interesting outcrop was identified in a carbonate quarry near Valdieri (north-west Italian Alps): it was studied coupling a geo-mechanical and a thermo-physical approach. In particular, geo-mechanical and photogrammetric surveys, InfraRed Thermography (IRT), and Thermal Conductivity (TC) measurements were conducted. The rationale of the research is based on the fact that, when a substantial temperature difference between flowing groundwater and rocks was detected, IRT can reveal information about geo-mechanical and hydrogeological properties of the rock masses such as a degree of fracturing and joint interconnection. A comparative field and laboratory analysis using different devices enabled a more detailed insight providing values in both dry and wet conditions. A different thermal response was highlighted for the cataclastic zone as well. IRT results showed an evident inverse relationship among the number of joints per meter and the detected surface temperature. This is probably caused by the higher water flow within the cataclastic fault zone. Moreover, low fractured portions of the rock mass presented higher cooling rates and conducted heat far more than those with poor geo-mechanical characteristics (difference up to 40%). A negligible ratio between wet and dried thermal conductivity (about 1%) was also detected in lab measurements, which confirmed that primary porosity is not usually relevant in influencing thermal properties of the sound rock. Full article