Search Results (39)

Background: Sleep apnea is a breathing affliction that affects sleep quality, with significant implications for overall physical and oral health, as well as mental health. Aim: The aim of this study was to evaluate the validity of the STOP-Bang and Epworth questionnaires as preoperative screening tools for obstructive sleep apnea (OSA) in the adult population from the NE region of Romania. Materials and Methods: A total of 222 participants were selected. The questionnaire method was used, with the subjects completing the STOP-Bang and Epworth questionnaires. A chi-squared test, an ANOVA, and Student’s t-tests were used for the statistical analysis. Results: the STOP-Bang questionnaire revealed an increased risk of OSA among those with a high BMI, an age over 50 years, or a large neck circumference. Regarding the Epworth questionnaire, daytime sleepiness was more frequent among obese individuals and those with associated pathologies. Significant correlations were found between OSA and obesity, age, and associated pathologies, with limited observations on the influence of gender on the risk of OSA. Conclusions: The studied questionnaires are effective and easy-to-use tools for the preoperative screening of OSA, demonstrating a significant correlation between the identified risk factors and the disease severity. Full article

Animal and plant remains recovered from two archaeological sites in Iași city (NE Romania) were analyzed in this work. The aim of this study was to contribute to the economic and environmental evaluation of an old urban settlement. The analyzed sites, both of value in archaeological preventive research, are dated to the 18th–19th centuries. The archaeozoological analysis consisted of anatomical, taxonomic, and taphonomic identifications, quantification, estimations of age at slaughter and sex, and osteometry. The results revealed that the animal remains are of domestic origin, as indicated by traces of butchering, and can be mainly described in terms of their frequencies (i.e., number of identified specimens and minimum number of individuals), selection for slaughter, morphology, and size. The results show that animal husbandry (e.g., cattle, sheep/goat, pig, and horse) had significant importance in the economy of the settlement, and cattle were preferred for consumption. Few remains were identified for wild mammals (i.e., red deer, wild boar, and hare), birds, and mollusks. The phytolith analysis indicated that some plant resources were used in the economy of this settlement. The identification of opal silica bodies revealed the presence of grasses and cultivated cereals. Full article

Background and Objectives: Antiretroviral therapy (ART) has revolutionized the management of HIV infection, transforming it from a once-debilitating disease to a chronic, manageable condition. However, challenges such as treatment resistance, medication side effects, and long-term tolerability persist, prompting the exploration of novel therapeutic approaches. We aimed to highlight the characteristics and related comorbidities of HIV/AIDS cases in which the antiretroviral therapy was modified. Material and Methods: A cross-sectional clinical investigation was conducted on adults diagnosed with HIV/AIDS who were hospitalized at the “St. Parascheva” Clinical Hospital of Infectious Diseases in Iasi in the Northeastern region of Romania. The timeframe under investigation was 1 January 2023 to 30 June 2023. Results: In the Northeastern part of Romania, from a total of 1692 patients in the active records, there were a total of 148 recorded cases of antiretroviral therapy switch in HIV-infected patients. The main reason for the ART switch was the simplification of the ART regimen (82 cases, 55.40%), viro-immunological failure (16 cases, 10.66%), other disturbances correlated to the ART regimen, dyslipidemia (34 cases 22.97%), depression (3 cases, 2.02%), suicide attempt (1 case, 0.67%), new situations, including the appearance of pregnancy (3 cases 2.02%), and tuberculosis (9 cases, 6.08%). ART before the switch was represented by protease inhibitors that accounted for 84 cases (56.75%) of the ART switch. Following the therapy switch, integrase inhibitor-based ART single-tablet regimens accounted for 43.91% (65 cases) of all changeovers, with non-nucleoside reverse transcriptase inhibitor regimens coming in second, in 63 cases, 42.66%. Conclusions: ART switch as an experimental therapy offers a promising approach to optimizing HIV treatment outcomes. By focusing on viral suppression and immune reconstitution, addressing treatment challenges, and exploring novel ARV agents, ART switch strategies aim to improve the overall health and well-being of individuals living with HIV. Full article

We analyzed mineralogical characteristics, and major as well as rare earth element concentrations, from a cryptotephra layer in sediments of the infilled maar of Auel (Eifel, Germany). The results of detailed geochemical analyses of clinopyroxenes and their glassy rims from the Auel cryptotephra layer showed that they are similar to those from the thick Campanian Ignimbrite tephra occurrence in a loess section at Urluia (Romania). Both tephras show idiomorphic green clinopyroxenes and formation of distorted grains up to millimeter scale. The cryptotephra in the Auel core has a modelled age of around 39,940 yr b2k in the ELSA-20 chronology, almost identical to the latest ⁴⁰Ar/³⁹Ar dates for the Campanian Ignimbrite/Y-5 (CI/Y-5) eruption. These observations suggest that parts of the CI/Y-5 ash cloud were transported also northwestward into Central Europe, whereas the main branch of the CI/Y-5 ash plume was transported from southern Italy towards the NE, E, and SE. Based on pollen analyses, we conclude there was no direct effect on vegetation from the CI/Y-5 fallout in the Eifel area. Trees, shrubs, and grasses remained at pre-tephra-airfall levels for roughly 240 years, but changed around 39,700 yr b2k when thermophilic woody plants (e.g., Alnus and Carpinus) disappeared and Artemisia spread. This change in vegetation was well after the Laschamp geomagnetic excursion and also after the GI9 interstadial and quite probably represents the onset of the Heinrich Event 4 (H4) cold spell, when climatic conditions over the North Atlantic, and apparently also in Central Europe, deteriorated sharply. Full article

Infectious and inflammatory dermatoses featuring skin lesions with loss of tissue expose skin layers to microbial invasions, disrupt the normal skin microbiome, and potentially lead to sepsis. However, literature data on the incidence of cutaneous-onset sepsis are scarce. This retrospective observational study assessed hospital admissions for primary skin lesions without bacterial infections and sepsis during 2020–2022 in the largest emergency hospital in NE Romania. Of 509 patients, 441 had infected lesions, 78 had sepsis caused by venous ulcers from microbial eczema cellulitis, superinfected bullous dermatoses, erysipelas, and erythroderma. Cultured samples revealed S. aureus, P. aeruginosa, and E. coli; and K. pneumoniae and S. β-hemolytic associated with sepsis, even if this was rarer. Clinical manifestations included ulcerations, erosions, fissures, excoriations, bullae, vesicles, pruritus, tumefaction, edema, fever, chills, pain, adenopathy, and mildly altered mental status. Underlying chronic heart failure, atrial fibrillation, anemia, and type-1 diabetes mellitus were comorbidities associated with infection and sepsis. Significant associations and risk factors, including their combined effects, are discussed to draw attention to the need for further research and adequate management to prevent sepsis in adult patients of any age presenting with infected skin lesions (especially cellulitis) and comorbidities (especially type 1 diabetes mellitus and anemia). Full article

Animal husbandry is one of man’s oldest occupations. It began with the domestication of animals and developed continuously, in parallel with the evolution of human society. The selection and improvement of goats in Romania was not a clearly defined objective until around 1980. In recent years, with the increasing economic value given to goats, breeding programs are becoming established. In Romania, a few goat genetic studies using microsatellites and mtDNA have been carried out; however, a systematic characterization of the country’s goat genomic resources remains missing. In this study, we analyzed the genetic variability of Carpatina goats from four distinct geographical areas (northern, north-eastern, eastern and southern Romania), using the Illumina OvineSNP60 (RefSeq ARS1) high-density chip for 67 goats. Heterozygosity values, inbreeding coefficients and effective population size across all autosomes were calculated for those populations that inhabit high- and low-altitude and high- and low-temperature environments. Diversity, as measured by expected heterozygosity (H_E), ranged from 0.413 in the group from a low-temperature environment to 0.420 in the group from a high-temperature environment. Within studied groups, the HT (high temperature) goats were the only group with a positive but low average inbreeding coefficient value, which was 0.009. After quality control (QC) analysis, 46,965 SNPs remained for analysis (MAF < 0.01). LD was calculated for each chromosome separately. The N_e has been declining since the time of domestication, having recently reached 123, 125, 185 and 92 for the HA (high altitude), LA (low altitude), HT (high temperature) and LT (low temperature) group, respectively. Our study revealed a low impact of inbreeding in the Carpatina population, and the N_e trend also indicated a steep decline in the last hundred years. These results will contribute to the genetic improvement of the Carpatina breed. Full article

Sclerotinia sclerotiorum (Lib.) de Bary (1884) is a fungal plant pathogen with worldwide distribution and a varying host range from different botanical families. It can cause damage to a large variety of crops such as sunflower, soybean, dry bean, canola, some vegetables, and ornamental plants. This article reports the occurrence of twig blight on the forsythia plant from the NE region of Romania. The disease was observed on Forsythia × intermedia Zab. plants from the Arboretum Park of the Iasi University of Life Sciences (IULS), located in Iasi City, Romania. Infected tissue was investigated through morphological characteristics using Sanger sequencing. Genomic DNA was extracted from the isolate obtained from naturally infected plants, and the ribosomal internal transcribed spacer region was amplified using the ITS1, ITS2, and LSU D1 and D2. Based on the results of this study, molecular and morphological data suggest that Forsythia twig blight can be caused by S. sclerotiorum. Constant monitoring of Sclerotinia sclerotiorum across multiple hosts and time intervals will reduce potential spread and future economic losses in cultivated species. Full article

Oil contamination in soil from a variety of sources, including accidental leaks, industrial activities, and improper waste disposal, involves disrupting ecosystems, contaminating water, harming human health, and diminishing agricultural productivity. Bioremediation is becoming the most important method accepted as a treatment for hydrocarbon-polluted soil using indigenous microbial flora, which aims to restore soils to their pre-hydrocarbon pollution characteristics. We will follow in our article to give some examples of good practices for bioremediation of oil-polluted soils in some sites in NE Romania. In a first step, all the oil extraction wells in NW Romania were mapped, both those in operation and those abandoned, reaching 695 wells. For 7 case studies, soil profiles were taken from the vicinity of the well column and from points close to it located in the well casing, for which the concentration of total petroleum hydrocarbons was determined. Using GIS spatial interpolation techniques, the theoretical concentration of petroleum hydrocarbons in the soil was determined. The polluted soil was transported to the bioremediation station, where it was exposed to bioremediation procedures, and the period and duration until the soil was brought to the accepted parameters in terms of allowable concentrations were analysed. The time required varies between 17 and 36 weeks needed for bioremediation. Following practical applications, it can be concluded that the time required for bioremediation is directly dependent on the initial concentration of pollutants and the number of chemical and physical interventions applied to the soil. Full article

The identification and delineation, through aerial photography, of the archaeological structures that present temporal resolution, as well as their characterization based on high-resolution LiDAR (Light Detection and Ranging)-derived DEMs (Digital Elevation Models) are modern techniques widely used in the archaeological prospecting of various landscapes. In this study, we present an application of Airborne Laser Scanning (ALS) and aerial photography (AP) techniques, used in order to compute geomorphometric indices specific to the ashmound structures of Late Bronze Age (LBA) archaeological sites that are visible on the soil surface. The necessity of determining the ashmounds’ geoarchaeological description stems from the fact that despite the majority of archaeologists weighing in on the subject, there is still no accepted explanation regarding their initial functionality. Thus, we believe that the GIS-based high-resolution characterization of 200 ashmound features identified in 21 Noua Culture (NC) archaeological sites will contribute to a better understanding of the ashmounds’ functionality and evolution in the heterogeneous landscape of the study area (NE Romania). Therefore, various shape indices, such as the area (A), perimeter (P), length (L), form factor (RF), circularity ratio (RC), and elongation ratio (RE) were computed for microlevel characterizations of the visible ashmounds’ structures. Additionally, LiDAR-derived DEMs with a 0.5 m resolution were used to generate more surface characteristics such as the slope (S) and hypsometric indices (HI). The outcomes indicate that the ashmounds have relatively diverse shapes (an RF range from 0.37 to 0.77; a RC range from 0.79 to 0.99; a RE range from 0.68 to 0.99), and the micro-relief slightly varies from positive to negative landforms (HI range from 0.34 to 0.61) depending on the erosion intensity (S range from 1.17° to 19.69°) and anthropogenic impact (e.g., current land use and agriculture type). Furthermore, each morphometric parameter is an indicator for surface processes, aiding in the identification of the geomorphologic and surface-erosion aspects that affect the archaeological remains, contributing to the assessment of the conservation status of the ashmound structures within the current landscape configuration. In this regard, this article presents and discusses the remote sensing (RS) techniques used, as well as the morphometric data obtained, exploring the implications of our findings for a better characterization of the NC in Romania. Full article

This study aims to contribute to the knowledge of the medieval Moldovan economy by evaluating animal resources (e.g., animal husbandry, hunting, fishing) based on the skeletal remains found in archaeologic sites from northeastern Romania and the Republic of Moldova. Animal remains, especially those from the urban settlement of the 14th–16th centuries from Târgu Neamţ (NE Romania), were described in terms of their frequencies (i.e., number of identified specimens and minimum number of individuals), morphometry, and livestock management (i.e., animal selection by age and sex). The results were compared with those obtained from other settlements—rural, urban, and fortress—from medieval Moldova. Correspondence analysis of the identified animals and settlements on the basis of the frequency values reveals associations between the two variables (animal species and settlement). Full article

The Coronavirus disease 2019 (COVID-19) pandemic has brought new challenges across medical disciplines, particularly in infectious disease medicine. In Romania, the incidence of SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) infection increased dramatically since March 2020 until March 2022. Antibiotic administration for pulmonary superinfections in COVID-19 intensified and, consequently, increased rates of Clostridioides difficile infection (CDI) were hypothesized. We conducted a single-center, retrospective, observational study on patients from North-Eastern Romania to assess clinical characteristics and outcomes of COVID-19 and Clostridioides difficile (CD) coinfection, and to identify risk factors for CDI in COVID-19 patients. The study enrolled eighty-six CDI and COVID-19 coinfected patients admitted during March 2020–February 2021 (mean age 59.14 years, 53.49% men, 67.44% urban residents) and a group of eighty-six COVID-19 patients. On admission, symptoms were more severe in mono-infected patients, while coinfected patients associated a more intense acute inflammatory syndrome. The main risk factors for severe COVID-19 were smoking, diabetes mellitus, and antibiotic administration. Third generation cephalosporins (55%) and carbapenems (24%) were the main antibiotics used, and carbapenems were significantly associated with severe COVID-19 in patients coinfected with CD during hospitalization. Coinfection resulted in longer hospitalization and poorer outcomes. The extensive use of antibiotics in COVID-19, particularly carbapenems, contributed substantially to CD coinfection. Full article

During the end of the Bronze Age, the territory of present-day eastern Romania was occupied by Noua communities, belonging to the Noua-Sabatinovka-Coslogeni (NSC) cultural complex. Although these communities have left us a large number of archaeological sites, this period is rather poorly known and understood, mostly because the investigation of Late Bronze Age (LBA) sites is very rare, usually consisting of small test trenches or fieldwalks. The main characteristic of these communities and the subject of our study is represented by the so-called ashmounds (grey, quasi-circular spots, visible on the soil surface, with small elevations and diameters of 25–30 m), present inside most settlements. Our paper aims at highlighting the spatial characteristics of these sites, using GIS (Geographic Information System) tools, as well as aerial photographs, LiDAR (Light Detection and Ranging) measurements, magnetometry and geo-electrical methods, in order to identify the relationship existing between Noua communities and the inhabited environment, in the area known as the Jijia River catchment. Thus, our approach was able to outline the way in which the geographical peculiarities determined the establishment of new settlements, revealing that the human groups from the end of the Bronze Age preferred low terrains with smooth slopes, located in the immediate vicinity of the most important watercourse of the inhabited micro-area. Additionally, our geophysical studies allowed us to confirm the lack of ash located within the ashmound, as well as to signal the possibility that these features have become visible on the soil surface only due to the irreversible damage caused by intensive agricultural processes. Despite the small number of excavations, to this day an important number of studies have been dedicated to the communities and features in question; however, no analysis has yet been performed that unites the tools specific to GIS software with the usage of non-invasive methods (such as aerial photographs, LiDAR measurements and geophysical techniques). Full article

This study aimed to evaluate the fatty acid profile and health lipid indices of sheep meat (from 52 Karakul sheep from NE Romania). The effect of age at slaughter and the influence of muscle region were studied for nutritional parameters, especially the fatty acids from lipid fractions. Based on the fatty acid profiles and lipid contents, the sanogenic indices were determined for two sheep muscle groups. Thus, two different muscle regions from lamb and adult sheep were analysed from both genders, the Longissimus dorsi and Triceps brachii, to argue the advantages of each category and the rationalization, in terms of meat consumption, regarding their impact on human health. Sheep meat has many components with beneficial effects on human health. Apart from the fact that it is an important source of nutrients due to its high content of proteins, lipids, and minerals, it is also a product that can provide fundamental bioactive compounds for maintaining metabolic functions. The qualitative indices assessment revealed that lambs have meat with high PUFA content on Longissimus dorsi muscles (approx. 25% of total fatty acids), 0.68 for PUFA/SFA, with highest values for n-3 (approx. 8%) and n-6 (approx. 14%). Appropriate values can also be observed in Triceps brachii muscles from adult sheep. The sanogenic indices also presented good values for Longissimus dorsi from lambs and Triceps brachii from adult sheep (polyunsaturation index = 7.2–10.2; atherogenic index = 0.56–0.67; thrombogenic index = 0.78–0.96; hypocholesterolemic/hypercholesterolemic index = 2.4–2.7 (for Longissimus dorsi)). Full article

Aims and Objectives: To investigate the trends in access to dental services among adults from the N-E region of Romania and to evaluate the factors that influence access to dental care. Material and Methods: A self-administered questionnaire was used to evaluate the access and utilization of dental care among a sample of adults. We collected data on their demographic characteristics, their type of medical insurance, their monthly income, the type of dental office they visited, their reasons for choosing a certain type of dentist office, and their oral hygiene habits. It was found that their financial status determined by the occupation of the individual, as well as their monthly income, influenced their access to medical services. Data were analyzed using SPSS 20.0. Results: Of a total of 696 participants, 55.6% were female, 83.3% were from urban areas, 42.1% were retired, 62.3% of the subjects utilized dental emergency services, and 67.9% of the subjects self-funded their treatment. The reasons for women not attending dental offices included costs (24.3%) and dental fear (9.8%), while men’s reasons were high costs (26.4%) and lack of time (5.9%). Significant differences were recorded between gender and reasons for visiting the dentist (p = 0.018), payment for dental services (p = 0.009), and preferred clinic (p = 0.010). In relation to occupation, there were significant differences for most of the variables evaluated (reasons to visit a dentist, payment for dental services, preferred dental clinic, self-rated oral health, etc.). Conclusions: Gender, occupation, and monthly income levels were found to influence access to dental medicine services of Romanians in the N-E region. Dental services were frequently accessed for emergency reasons. Dental services were found to be paid for by state insurance for those with low monthly incomes and self-funded for those with higher monthly incomes. Full article

In the present study, the salinization trends of different soil types from a small hydrographic basin situated in NE Romania (Roșior basin) are investigated. The climatic conditions are favorable for long dry summers and intensive evaporation. The soils are developed on immature evolved clay sediments, as revealed by geochemical analysis. The salinity varies among soil types, attaining maximum values for Solonetz with total dissolved salts (TDS) between 1165.75 to 1881.25 mg/L. The aqueous solutions are represented by natural water and soil solution. The hydrogeochemical facies of the natural waters change from HCO₃⁻—Mg²⁺ in the upper basin to SO₄²⁻—Na⁺ in the middle or lower basin. The soil solution is moderately or strongly salinized and shows anionic variations from HCO₃⁻ to SO₄²⁻ in the studied profiles, whereas Na⁺ is always the main cation. The concentrations of Na⁺ and SO₄²⁻ evolve simultaneously. Raman spectroscopic exploration of the white efflorescences, which occur on topsoil, reveals the presence of thenardite as the dominant phase. The composition of soil solution results from both the ionic exchange and evaporation processes. The nature of soil solution mineralization and summer temperatures are two main factors that interact and promote the thenardite precipitation. The soil salinization induces negative effects on crop nutrition, impacting further the crop yields. The results of this study can be extrapolated to larger areas formed on Sarmatian sedimentary deposits affected by salinization processes. Full article