Search Results (6)

This study investigates the presence of mineral oil hydrocarbons (MOHs) in corn silage, aiming to assess contamination levels and identify potential sources, including technological and environmental factors. Given the increasing concern regarding the presence of MOHs all over the food chain, this research provides important data on feed safety. A total of 15 corn silage samples were collected from the feed base of a dairy farm. Sampling was performed systematically across silos (top, middle, bottom layers). The analysis was conducted using LC-GC-FID to quantify mineral oil saturated hydrocarbon (MOSH) and aromatic hydrocarbon (MOAH) fractions. Statistical evaluation was applied to determine contamination patterns and potential influencing factors. The findings confirmed the presence of MOSH and MOAH in the analyzed silage, averaging 23.3 mg/kg MOSH and 1.4 mg/kg MOAH, exceeding European Commission guideline limits. Notably, the MOAH fraction, known for its potential toxicity, was detected at significant levels in several samples. The study highlights that corn silage can act as a source of MOSH/MOAH contamination in livestock feed. Technological processes, especially mechanized harvesting and ensiling, and environmental pollution factors appear to be likely the main contributors, emphasizing the need for improved monitoring and preventive measures to mitigate risks in the feed-to-food chain. Full article

Legislative frameworks about contamination are often limited to foods and underestimate the role of animal nutrition for safe production. This study aims to assess mineral oil hydrocarbon (MOH) contamination in feed and identify the technological factors that are contributing to this issue, particularly focused on mechanised harvesting and processing. Three dairy farms, classified by contamination risk (low, medium, and high), were selected, and fifteen feed samples were analysed using the coupled liquid chromatography–gas chromatography–flame ionisation detection (LC–GC–FID) method, with a microwave-assisted saponification (MAS) step to determine mineral oil saturated (MOSH) and aromatic (MOAH) hydrocarbon levels. Important contamination levels were observed depending on the technological development of each farm. MOSH levels ranged from 11.4 mg/kg to 81.40 mg/kg, while MOAH levels ranged from 0.5 mg/kg to 4.6 mg/kg. MOAH accounted for 4.74% of the total MOH content. The results showed a connection between feed production technologies and MOH contamination levels. Factors such as the mechanisation, the machinery used, and the storage conditions were potentially contributors to contamination, while chemical treatments had no direct impact but some potential risks. The contamination levels varied across farms, indicating certain contamination sources beyond technological factors. Advanced technological measures and proper equipment maintenance are suggested to mitigate MOH contamination risks in feed. Full article

This study assessed saturated mineral oil hydrocarbons (MOSH) and aromatic mineral oil hydrocarbons (MOAH) levels in grower–finisher feeds for pigs supplemented with 5% crude palm oil (CP), crude olive pomace oil (COP), olive pomace acid oil (OPA), or a blend of CP and OPA (50:50, w/w); the contribution of the lipid source to that contamination; and the ability of pigs to accumulate MOH in back fat and loin tissues after 60 days of trial. MOSH and MOAH were analyzed with liquid chromatography (LC)–gas chromatography (GC)–flame ionization detection (FID) after sample preparation. Among the lipid sources, CP had the lowest MOH levels, but CP feeds showed the highest contamination. This, along with the different MOSH profiles, indicated the presence of more significant contamination sources in the feeds than the lipid source. The higher MOH contamination in CP feeds was reflected in the highest MOSH levels in pig back fat, whereas MOAH were not detected in animal tissues. Also, MOSH bioaccumulation in pig tissues was influenced by the carbon chain length. In conclusion, feed manufacturing processes can determine the MOSH contamination present in animal adipose tissues that can be included in human diets. Full article

The aim of this work was to understand the actual content of mineral oil hydrocarbons (MOH) in olive pomace oil in order to contribute to the monitoring requested by EFSA for the food groups making a relevant impact on human background exposure. Such information will complement both the information inferred from the limits established by the EU and the interpretation of the coming toxicological risk assessment. At the same time, the origin of such a group of compounds is discussed. From the raw material to the commercial product, olive pomace oils were sampled and analyzed at different points and/or conditions. Through the ultimate online HPLC-GC-FID system, we gathered information on the MOH concentrations and molecular mass profiles (C-fractions), and through GCxGC-TOF/MS, we identified the key structures that prove the innocuousness of the mineral oil aromatic hydrocarbon (MOAH) fraction. Our approaches provided chromatographic signals on the C10-C50 range, rendering 33–205 mg/kg mineral oil saturated hydrocarbon (MOSH) and 2–55 mg/kg MOAH in the commercial product. The results confirmed that the C25-C35 cut is the main fraction to which humans are exposed via olive pomace oil, showing concentrations highly dependent on the extraction process. Moreover, the identification of the main MOAH groups showed that in olive pomace oil, mainly 1- and 2-ring species were present, being virtually free of the carcinogenic 3–7 ring aromatics. Full article

A method was developed for the determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) in edible oils, achieving similar limits of quantification than those obtained by online extraction methodologies, i.e., 0.5 mg/kg. The isolation of MOSH and MOAH was performed in a silver nitrated silica gel stationary phase prior to their analysis by gas chromatography–flame ionization detector (GC-FID). To improve the sensitivity, the simulated on-column injection method, using a suitable liner, was optimized. The method was validated at 0.5, 10.0 and 17.9 mg/kg, and recoveries ranged from 80 to 110%. Intra and inter-day precision were evaluated at the same levels, and relative standard deviation (RSD) was lower than 20%. The method was applied to a total of 27 samples of different types of oil previously analyzed in an accredited laboratory, detecting MOSH up to 79.2 mg/kg and MOAH up to 22.4 mg/kg. Full article

Paper-based food packaging materials are widely used, renewable, and biodegradable. Because of its porous structure, paper has poor or no barrier performance against grease, water vapor, water, and volatile organic compounds. Moreover, recycled paperboard can be a source of organic residuals that are able to migrate into packed food. Two different types of paperboard produced from primary and secondary cellulosic fibers were coated using renewable materials, such as alginate and chitosan, and comprehensive barrier measurements showed multifunctional barrier properties of these two biomaterials. Both paper substrates were successfully coated using a draw-down coater, and the measured air permeability of the coated samples was 0 mL·min⁻¹. Grease resistance was improved, while it was possible to reduce water vapor transmission, the migration of mineral oil saturated hydrocarbons and mineral oil aromatic hydrocarbons (MOSH/MOAH), and the permeation of volatile compounds for both paper substrates when compared with uncoated substrates. Wettability and water absorptiveness of chitosan- and alginate-coated papers were found to be substrate-dependent properties, and could be significantly affected by bio-based coatings. In summary, industrially produced paperboard was upgraded by coating it with the naturally biodegradable biopolymers, alginate and chitosan, thus achieving extraordinary barrier performance for various applications within the packaging industry. Full article