Search Results (989)

Despite substantial advances in our understanding of cancer metastasis, it remains the leading cause of mortality among cancer patients. Elucidating the molecular mechanisms that drive metastatic progression is expected to facilitate the development of more effective therapeutic strategies. Among the numerous candidates, the long non-coding RNA H19 and its derivative miR-675 have been increasingly recognized as key regulators of metastatic dissemination in cancers of diverse tissue origins. In this review, we provide an up-to-date overview of the H19/miR-675 axis in metastatic progression, with particular emphasis on its involvement in the dynamic and complementary processes of epithelial–mesenchymal transition (EMT) and mesenchymal–epithelial transition (MET). We also highlight the opportunity to consider the H19/miR-675 axis as promising biomarkers and potential therapeutic targets. Full article

Sarcocystis, a diverse and species-rich protist genus infecting reptiles, birds, and mammals, remains poorly understood in terms of true diversity and their lifecycles. Typically, sarcocysts are found in the muscle tissue of the intermediate host (IH), while oocysts undergo sporulation in the intestines of the definitive host (DH). Rodent-associated Sarcocystis species often form cryptic species complexes with strong specificity to their DHs; however, their presence in the intestines of wild carnivores, whose IHs are rodents, is understudied. The aim of this study was to investigate the distribution of rodent-associated Sarcocystis species in the intestines of wild Mustelidae and Canidae from Lithuania using light microscopy (LM) and nested PCR targeting 28S rRNA. LM analysis of intestinal scraping revealed Sarcocystis spp. in 56.3% of canids and mustelids, while DNA sequence analysis identified 41.0% of mustelids and 11.6% of canids as positive. Three Sarcocystis species, S. arvalis, S. myodes, and S. ratti, and the genetic lineage Sarcocystis sp. Rod8, which belong to the same cryptic species complex, were identified in mustelids, while S. arvalis and S. myodes were detected in canids. Thus, mustelids contribute more than canids to the natural transmission of Sarcocystis spp. from rodents in Lithuania. Full article

Multimodal high-resolution ultrasound, including B-mode, Power Doppler, MicroVessel Doppler, spectral Doppler, and strain elastography, was used to assess concomitant dactylitis and hidradenitis suppurativa (HS) in a 46-year-old woman with severe hidradenitis suppurativa (IHS4 = 28), who was diagnosed 1.5 years ago and has been using adalimumab. Axillary ultrasound demonstrated abscess cavities and draining fistulous tracts with marked structural distortion, increased vascular signal on advanced Doppler modalities, and heterogeneous stiffness patterns on elastography, consistent with active deep inflammatory involvement. Simultaneously, evaluation of the third right finger revealed flexor tendon sheath thickening, soft-tissue edema, Doppler-positive inflammatory activity, and altered biomechanical properties compatible with dactylitis. High-resolution ultrasound has been increasingly recognized as a valuable tool for evaluating inflammatory and structural changes in cutaneous diseases, including HS. These multimodal findings illustrate how structural, vascular, and biomechanical ultrasound parameters may provide complementary information for characterizing inflammatory tissue remodeling in HS associated with dactylitis. As this report describes a single patient, these elastographic observations should be considered exploratory and hypothesis-generating rather than evidence of clinical validation. Full article

Maritime transport is essential for global trade, yet ship emissions remain a major source of air pollution in coastal and port areas, with potential impacts on local air quality and human health. Cruise ships are particularly relevant in urban ports because, beyond propulsion, they require a continuous onboard energy supply for hotel services while berthed. This study develops a bottom-up emission inventory for cruise ship calls at Istanbul Galataport during the 2024 season, estimating CO₂ as a greenhouse gas (GHG) and NO_x, SO_x, and particulate matter (PM) as air-quality pollutants generated during manoeuvring and hotelling phases. Ship technical characteristics (engine type, installed main and auxiliary power, engine speed class, and year of build) were obtained from the IHS database, while port call activity data were provided by the terminal operator. Emission factors were primarily based on the IMO Third Greenhouse Gas Study and complemented with established literature sources to address missing vessel information and ensure methodological consistency. Results indicate that hotelling dominates total emissions, reflecting the high auxiliary power demand during berths. Results show that total annual emissions from 164 cruise ship calls amount to approximately 31,360 t·y⁻¹ of CO₂, 370 t·y⁻¹ of NO_x, 350 t·y⁻¹ of SO_x, and 44 t·y⁻¹ of PM. Hotelling operations account for the dominant share of emissions, contributing more than 90% of total CO₂ and the majority of NO_x and SO_x emissions, due to sustained auxiliary engine demand during berth stays. These findings confirm that cruise ship activity represents a significant localized emission source in densely populated port environments and provide a quantitative baseline for evaluating mitigation measures such as shore power, cleaner fuels, and operational strategies aimed at reducing at-berth emissions. Full article

Background: Iron deficiency remains a major nutritional challenge, partly due to the limited stability and bioavailability of conventional iron formulations in foods and during digestion. In this study, iron–protein microparticles (IP-MPs) based on bovine serum albumin (IA-MPs) and hemp protein (IH-MPs) were developed via coprecipitation and evaluated as food-compatible iron delivery systems. Methods: Iron–protein microparticles (IP-MPs) were fabricated by a coprecipitation technique. The stability of IP-MPs was investigated in a three-phase digestion model. The uptake of IP-MPs by Caco-2 cells as well as the Ferritin concentration in Caco-2 cells were investigated. Results: Particle morphology and size distribution were strongly dependent on the protein matrix, with hemp protein microparticles exhibiting greater size uniformity and higher stability under simulated gastric conditions. In a standardized in vitro gastrointestinal digestion model, both IP-MP formulations preserved iron predominantly in the bioactive Fe(II) state and remained sufficiently intact to reach the intestinal phase. Biocompatibility and iron uptake were assessed using Caco-2 cell monolayers. Neither formulation induced cytotoxic effects, while iron delivered via IP-MPs showed enhanced cellular uptake compared to a commercial iron supplement and ferrous sulfate. The amount of Fe(II) detected in the basolateral compartment of IH-MP and IA-MP samples (1.4 µg and 1.3 µg, respectively) was higher than that observed for Floradix^® samples (approximately 0.7 µg) and corresponded to about 25% of the total iron applied. Functional iron bioavailability, assessed by ferritin formation, was significantly higher for IP-MPs, with hemp protein microparticles yielding the strongest ferritin response. Conclusions: These results demonstrate that iron–protein microparticles, particularly those based on hemp protein, effectively improve iron stability during digestion and enhance cellular iron bioavailability, highlighting their potential for application in iron fortification and functional food systems. Full article

Serotonergic projections innervate both the dorsal and ventral cochlear nuclei; however, the electrophysiological consequences of serotonergic input in the ventral cochlear nucleus (VCN) remain incompletely understood. This study aimed to identify the serotonin receptor subtypes involved in serotonergic modulation of stellate cells in the mouse anteroventral cochlear nucleus (AVCN) and to determine the underlying ion channel mechanisms. Whole-cell patch-clamp recordings were performed in acute brain slices obtained from postnatal day 12–17 mice. Bath application of serotonin (25 µM) induced membrane depolarization (~5 mV) and increased action potential firing. Pharmacological experiments demonstrated that antagonists of 5-HT1A, 5-HT2A, and 5-HT2C receptors partially reversed the depolarization and reduced serotonin-induced inward currents, indicating that multiple receptor subtypes contribute to serotonergic excitation. Blockade of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels with extracellular Cs⁺ suppressed approximately 95% of the serotonin-induced depolarization and inward current, implicating HCN channel-mediated Ih as a principal ionic mechanism. Serotonin significantly increased Ih amplitude. Analysis of steady-state activation revealed no statistically significant shift in V0.5; however, under near-resting membrane potential conditions, serotonin significantly reduced the slope factor of the activation curve, consistent with altered voltage sensitivity of Ih gating. Immunohistochemical analysis confirmed the presence of 5-HT1A, 5-HT2A, and 5-HT2C receptors in the AVCN. Together, these findings indicate that serotonergic excitation of AVCN stellate cells is mediated by coordinated activation of multiple 5-HT receptor subtypes and primarily involves modulation of HCN-dependent subthreshold membrane dynamics. Full article

The guinea fowl (Numida meleagris), a thermo-tolerant and disease-resilient poultry species, holds great potential for sustainable poultry production in climate-vulnerable regions. The genomic aspects of this species remain largely understudied. The present study aims to delineate the patterns of domestication and understand the evolutionary dynamics of guinea fowl populations (wild and domestic) across three continents, utilizing whole-genome sequencing data from 122 genomes. The population structure analyses (ADMIXTURE, PCA, phylogeny, F_ST, LD, and MAF) revealed that Indian guinea fowl (CARI) shared close ancestry with Iranian (IRAD) and Chinese (CHID) domesticated populations while remaining distinct from wild lineages. The runs of homozygosity (ROH) identified 49,088 segments, with short fragments (ROHs) preponderant in Indian and domestic populations, reflecting historical inbreeding and effects of domestication cum selection. Copy number variation (CNV) analysis revealed 105,178 CNVs concatenated into 40,067 CNV regions (CNVRs) across 11 populations, establishing the first CNV atlas for guinea fowl at the global level. Gene annotation of overlapping ROH and CNVRs revealed 1080 common candidates across Asian guinea fowl populations, i.e., the Indian guinea fowl (CARI), IRAD, and CHID, including FOS, EPAS1, CD74, and CSF1R. These genes have earlier been associated with immune regulation, stress response, and thermal adaptation. Selection signature scans, integrating intra-population (iHS) and inter-population (XP-EHH) approaches, uncovered genes under positive selection linked to immune response (like BCL11B, IL18, and GPC3), thermo-tolerance (like TRPV4 and BAG3), lipid metabolism (like AACS and ELOVL4), and pigmentation (BCO2). These signatures highlight the molecular basis of resilience in guinea fowl and their potential to withstand climate-induced stresses. This study presents the first global CNV atlas for guinea fowl and provides the first comprehensive genomic characterization of the Indian domestic population, integrating ROH, CNV, and selection signature analyses. It offers a comprehensive assessment of guinea fowl genomes (wild and domesticated) across three continents, offering insights into domestication, evolutionary dynamics, and the genetic basis of their adaptation and resilience. Full article

Background: Tessadori–Bicknell–van Haaften syndrome (OMIM #619759) is a rare autosomal dominant neurodevelopmental disorder associated with heterozygous variants in genes encoding histone H4 proteins. The condition is characterized by global developmental delay, craniofacial dysmorphism, hypotrophy, intellectual disability, and ophthalmologic anomalies. More than 30 individuals with variants in histone H4 genes have been reported to date, reflecting the genetic heterogeneity of this emerging disorder. According to OMIM, the association between the H4C11 gene and Tessadori–Bicknell–van Haaften syndrome 2 is currently considered provisional. Methods: We report the case of a 5-year-old female presenting with expressive language delay, social interaction difficulties, and craniofacial features including microcephaly, exophthalmos, and periorbital fullness (“puffy eyes”). Family history revealed two sisters with borderline intellectual functioning who have not undergone genetic testing. The patient’s father carried the same heterozygous H4C11 variant (c.97C > T), while maternal testing was negative. Results: Neuropsychological evaluation revealed borderline intellectual functioning (IQ 73 at first assessment, 85 at follow-up) with persistent expressive language impairment. Ophthalmologic examination confirmed congenital exophthalmos and hypermetropic astigmatism. Laboratory investigations showed low ferritin and mildly elevated TSH levels, which may have contributed to the observed growth delay. At follow-up, the patient showed an increase in IQ score (73 to 85); however, test–retest variability cannot be excluded. Conclusions: This case highlights the importance of careful clinical assessment and cautious interpretation of genetic findings in children with neurodevelopmental delay. Familial segregation of a variant of uncertain significance (VUS), in the absence of functional evidence, should be interpreted conservatively and integrated with detailed phenotypic evaluation to guide clinical management and follow-up. Full article

As an elite indigenous poultry breed under national protection in China, the Xupu goose is renowned for its large body size, superior fatty liver production, premium meat quality, and high tolerance to roughage. To elucidate its genomic architecture, genetic diversity, and evolutionary selection signatures, we conducted whole-genome resequencing on 15 purposively selected, unrelated male Xupu geese. An average of 6.79 Gb of high-quality sequence data was generated per individual, yielding approximately 4.27 million single-nucleotide polymorphisms (SNPs) with a transition/transversion (Ti/Tv) ratio of 2.49. Population genomic analyses revealed that while the population retains a moderate genetic reservoir (H_E = 0.298), it exhibits a distinct heterozygote deficit (H_O = 0.217) and a moderate genomic inbreeding coefficient F_ROH = 0.204). This structural pattern underscores the genetic impact of historical ex situ closed-flock conservation and the consequent formation of cryptic family lineages. Furthermore, genome-wide integrated haplotype score (iHS) scans detected distinct regions under recent positive selection. Functional annotation of these regions highlighted candidate genes tightly associated with the breed’s hallmark traits, specifically lipid metabolism and hepatic fat deposition (ACSS2, ACSS3, PECR), alongside muscle development (CMYA5, MTPN, LEPR). Conclusively, this study delineates a comprehensive genomic landscape of the Xupu goose, providing a robust foundational resource for future germplasm conservation, molecular marker development, and precision breeding programs. Full article

Impatiens hawkeri W. Bull (I. hawkeri) is popular among consumers due to its diverse flower colors and year-round blooming. However, changes in ecological conditions, cultivation methods, and planting scale have led to increased disease incidence and diversity, particularly the widespread and destructive leaf spot disease. Currently, studies addressing the pathogen species and its biological characteristics remain limited. In this study, a highly pathogenic strain (IH-4) was selected from previously isolated fungi associated with leaf spot in I. hawkeri. Its taxonomic status was confirmed using upright fluorescence microscope analysis, internal transcribed spacer (ITS)/large subunit (LSU)/RNA polymerase II second largest subunit (rpb2)/β-tubulin (tub2) rRNA gene sequencing, and phylogenetic tree construction. Additionally, the biological characteristics of the pathogen and its sensitivity to 8 chemical fungicides were assessed. Strain IH-4 was identified as Ectophoma multirostrata (E. multirostrata) through combined morphological and molecular approaches. Optimal growth conditions included a temperature of 25 °C, a pH of 7, Potato Dextrose Agar (PDA) medium, fructose as the optimal carbon source, and urea as the optimal nitrogen source, with the fastest growth observed under a semi-light photoperiod (12 h light/12 h dark). Fungicide sensitivity assays indicated that 25% azoxystrobin exhibited the lowest half-maximal effective concentration (EC₅₀, 0.0724 μg/mL) and the steepest virulence regression slope (1.7), demonstrating the strongest inhibitory activity and highest sensitivity. Microscopic observations revealed that IH-4 hyphae penetrate I. hawkeri leaf tissues via stomata, colonize internally, and consequently cause host damage. This study provides a theoretical foundation for the timely and effective management of leaf spot disease in I. hawkeri. Full article

The reaction of the pyridylalcohol Ph₂C(OH)CH₂-2-py-6-Me (IH) with Me₃Al in refluxing toluene led to the isolation of the dimer [AlMe₂(μ-OC(Me)Ph₂)]₂ (1), whilst at ambient temperature the complex [(I)AlMe₂]·MeCN (2·MeCN) was isolated. Complex 1 is also readily available via the interaction of diphenylethanol and Me₃Al. Similar treatment of iPr₂C(OH)CH₂-2-py-6-Me (IIH) at ambient temperature afforded [(II)AlMe₂] (3). Treatment of IH and IIH with [VO(OiPr)₃] led to oxo-bridged complexes of the type [(VO)(μ₂-O)(I/II)]₂ (I (4·0.67MeCN), II (5)). The molecular structures of 1–5 are reported. These complexes have been employed as catalysts for the ring-opening polymerization (ROP) of the cyclic esters ε-caprolactone (ε-CL) and δ-valerolactone (δ-VL). For aluminium, complex 1/BnOH produced medium- to high-molecular-weight (M_n) PCL at 20 to 110 °C in solution, though some bi-/multi-modal behaviour was observed; for melts the M_n values were toward the lower end. For complexes 2 and 3, far lower M_n values for PCL were observed at 20 °C in solution and as melts, whilst in solution at 110 °C higher M_n values were achieved, though with less control. In general, M_n values for the PCL obtained using the vanadium complexes were low (≤8560 Da for 4, ≤2920 Da for 5). In the case of PVL, 1/BnOH in solution exhibited higher M_n values at lower temperatures with good control, and when employed as a melt, the M_n was toward the higher end (30,830 Da) observed. For 2/BnOH, much lower M_n values (≤2740 Da) were recorded both in solution and as a melt, whilst for 3, high M_n values were only observed in the absence of BnOH. Low M_n values (≤2920 Da) were also observed for the vanadium complexes 4 and 5. Kinetic results (both ε-CL and δ-VL) revealed that the vanadium complexes, particularly 4, outperformed the aluminium complexes. MALDI-ToF spectra revealed the formation of linear PCL polymers with BnO/H end groups for the aluminium/BnOH complexes in solution, and cyclic polymers when employed as melts. For vanadium, cyclic PCL polymers were the major family present. In the case of PVL, linear (BnO/H end groups) and cyclic polymers were observed when employing the Al/BnOH systems, whilst cyclic polymers were observed for vanadium. Copolymerization of ε-CL and δ-VL using 4/BnOH at 110 °C over 24 h led to incomplete conversion and formation of a random-type copolymer. Full article

Background and Clinical Significance: Foreign body ingestion represents an endoscopic emergency, with a risk of organ perforation of up to 35%, where increased prevalence was noticed among people with mental disorders and institutionalized patients. Case Presentation: The patient—male, 23 years old, and institutionalized for sequelae of infantile encephalopathy—was admitted for epigastric pain and hyperemetic syndrome that began 10 days earlier. Endoscopically, 12 hard plastic foreign bodies with sharp edges and sizes of 6–7 cm were identified, followed by extraction that was successfully performed in two sessions using a polypectomy snare and a Foreign Body Hood Protector. Additionally, multiple sessile exulcerated polypoid lesions were observed, measuring around 1–3 cm each, occupying the entire antrum. Histological examination showed inflammatory/regenerative elements, with features of moderate-to-high-grade dysplasia, while a rapid urease test for Helicobacter pylori infection was positive. As a consequence, the patient was administered triple eradication therapy. In addition, the patient presented marked features of hypereosinophilia and splenomegaly. Upon endoscopic reevaluation after 3 years and 8 months, no polyps were present and the H. pylori test was negative, while a complete and spectacular remission of both the hypereosinophilia and splenomegaly was observed. Conclusions: This case illustrates that the development and progression of gastric polyposis may be caused by the coexistence of chronic mucosal irritation from foreign bodies and H. pylori infection, which is a rare association. H. pylori eradication and endoscopic removal of the foreign bodies resulted in significant mucosal improvement. Full article

High-fidelity acquisition of canopy phenotypic data is critical for the advancement of orchard Artificial Intelligence (AI). Yet, an improper Light Detection and Ranging (LiDAR) installation height (IH) frequently induces data occlusion and substantial measurement errors. To address this limitation, this study developed an information collection vehicle (ICV) integrated with a 16-channel three-dimensional (3D) LiDAR to determine the optimal LiDAR IH. Three representative LiDAR IHs (1.4 m, 2.0 m, and 2.6 m) were evaluated on spindle-shaped cherry trees under both forward and reverse driving strategies. Subsequently, a novel 12-zone refined evaluation framework was introduced to quantify localized errors that are conventionally obscured by traditional whole-canopy metrics. Results demonstrated a profound nonlinear relationship between IH and measurement accuracy. Specifically, the 2.0 m IH (approximating the canopy’s geometric center) emerged as the optimal setup, maintaining relative errors (REs) below 5% with minimal dispersion. Conversely, the 2.6 m IH caused lower-canopy volume REs to surge beyond 16% owing to restricted downward viewing angles. Additionally, reverse driving at higher IHs exacerbated mechanical vibrations via the “lever arm effect”, thereby significantly degrading point cloud registration accuracy. Ultimately, these findings underscore the critical necessity of aligning sensors with the canopy geometric center, supplying essential theoretical guidelines for the hardware design of future orchard robots. Full article

This study pioneers the application of an induction heating fluidized bed (IH-FB) to dry methane reforming (DRM), establishing an efficient novel process for CO₂ utilization. Synergistic induction heating is achieved by utilizing eddy-current loss heating in a carbon steel rod for indirect heat transfer to particles and gases, coupled with hysteresis loss heating in magnetic Ni- and Co-based catalyst bed materials for direct induction heating. The system achieved an overall bed heating rate of 200 °C/min under fluidized conditions. DRM tests show that the IH-FB initiates catalytic reactions at a relatively low temperature of 400 °C, converting CH₄ and CO₂ into syngas (CO and H₂). Co-based catalysts exhibited higher feedstock conversion and enhanced stability compared to Ni-based catalysts owing to their greater hysteresis heating capacity and broader ferromagnetic temperature range, achieving 89.69% CH4 and 83.37% CO₂ conversions at 700 °C. Throughout the tested temperature range (400–700 °C), the IH-FB outperformed the resistance heating fluidized bed (RH-FB) in feedstock conversion, primarily due to its rapid thermal response, particle self-heating, and enhanced heat and mass transfer advantages from fluidization. At equivalent target conversion rates, the IH-FB significantly reduced the operating temperature compared to the RH-FB, demonstrating superior energy-saving benefits. This study demonstrated a promising route for efficient CO₂ utilization via DRM. Full article

Aminoglycosides remain important components of combination therapy for complicated Klebsiella pneumoniae infections in Vietnam; however, gene-level evidence linking aminoglycoside-modifying enzymes (AMEs) with minimum inhibitory concentrations (MICs) and multidrug resistance is limited, particularly in tertiary-care settings in southern Vietnam. A cross-sectional analysis was conducted on 186 non-duplicate aminoglycoside-resistant Klebsiella pneumoniae clinical isolates collected in a tertiary-care hospital in Ho Chi Minh City. Species identity was reconfirmed using ZKIR qPCR. MICs for amikacin, gentamicin, and tobramycin were determined by broth microdilution according to Clinical and Laboratory Standards Institute (CLSI) guidelines, and 14 AME genes were detected using targeted qPCR. Associations between AME genes, aminoglycoside MICs or susceptibility categories, and co-resistance to major antibiotic classes were assessed using non-parametric and exact tests with Benjamini–Hochberg false discovery rate correction, with emphasis on effect direction and clinically interpretable genotype–phenotype patterns beyond statistical significance alone. AME genes were highly prevalent, with ant(2″)-Ia, aac(6′)-Ir, and aac(6′)-Ib detected in 97.3%, 91.9%, and 89.8% of isolates, respectively. The presence of aac(6′)-Ib and aac(6′)-Ih_v was associated with higher aminoglycoside MICs, resistance to amikacin and tobramycin, and broader multidrug resistance, including carbapenem resistance, whereas several other AMEs were linked to lower MICs. These findings indicate that specific AME profiles, particularly aac(6′)-Ib and aac(6′)-Ih_v, are associated with intensified aminoglycoside resistance in this setting and support the need for gene-informed surveillance to prioritise confirmatory MIC-based Antimicrobial Susceptibility Testing (AST) to guide local antimicrobial stewardship. Full article