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21 pages, 3030 KB  
Article
Streptococcus dysgalactiae subsp. equisimilis from Diseased Pigs Are Genetically Distinct from Human Strains and Associated with Multidrug Resistance
by Fengyang Hsu, Kayleigh Gauvin, Kevin Li, Julie-Hélène Fairbrother, Jared Simpson, Marcelo Gottschalk and Nahuel Fittipaldi
Microorganisms 2026, 14(1), 9; https://doi.org/10.3390/microorganisms14010009 - 19 Dec 2025
Viewed by 544
Abstract
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has historically been recognized as a human pathogen, yet β-hemolytic streptococci consistent with SDSE have been documented in pigs for nearly a century. To investigate the population structure of porcine SDSE and the phylogenetic relationships between swine and [...] Read more.
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has historically been recognized as a human pathogen, yet β-hemolytic streptococci consistent with SDSE have been documented in pigs for nearly a century. To investigate the population structure of porcine SDSE and the phylogenetic relationships between swine and human strains, we characterized 41 isolates recovered from diseased pigs in Quebec, Canada (2019–2022). Infected animals spanned all major production stages and frequently presented with invasive disease, including arthritis, endocarditis, and sudden death. Core-genome phylogenetics resolved two heterogeneous porcine clades separated by long internal branches and clearly distinct from dominant human SDSE lineages. Most porcine isolates were emm-negative or contained structurally altered emm regions compared with human strains. Analysis of Lancefield antigen loci identified a predominant group C lineage and a minority group L lineage, recapitulating historical serogroup distributions described since the early-20th century. Phenotypic testing showed susceptibility to β-lactams and florfenicol but high levels of resistance to tetracycline, macrolides and lincosamides. Detected antimicrobial resistance (AMR) genes correlated well with phenotypes, and multidrug resistance was frequent. Hybrid genome assemblies revealed integrative and mobilizable elements carrying AMR determinants. Collectively, our data indicate that porcine SDSE represents a long-standing, genetically structured, host-adapted population with notable AMR potential, underscoring the need for continued swine SDSE genomic surveillance. Full article
(This article belongs to the Section Veterinary Microbiology)
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34 pages, 10060 KB  
Article
Regional Seismicity of the Northeastern Tibetan Plateau Revealed by Crustal Magnetic Anomalies
by Guoming Gao, Yecheng Li, Guofa Kang, Chunhua Bai and Limin Wen
Appl. Sci. 2025, 15(8), 4331; https://doi.org/10.3390/app15084331 - 14 Apr 2025
Cited by 2 | Viewed by 1254
Abstract
The northeastern Tibetan Plateau (NETP) is located at the front of the northeastward expansion of the Tibetan Plateau and is a tectonically active region with complex faults and intense seismicity. In this study, based on the high-order geomagnetic field model EMM2017, the crustal [...] Read more.
The northeastern Tibetan Plateau (NETP) is located at the front of the northeastward expansion of the Tibetan Plateau and is a tectonically active region with complex faults and intense seismicity. In this study, based on the high-order geomagnetic field model EMM2017, the crustal magnetic anomalies and Curie point depths (CPDs) in the NETP and adjacent areas were investigated. The relationship between the magnetic anomalies, CPDs, and seismic activity was assessed. The results show that strong earthquakes occur mainly in areas where the magnetic anomalies are negative or have a strong-to-weak transition. The CPD is located at 18–42 km. In the NETP, a shallow CPD corresponds to high heat flow. In contrast, in surrounding areas, a deep CPD corresponds to low heat flow. The northeast area from Bayan Har to the Qilian orogenic belt, and the region with a deep CPD in the Qaidam Basin, record the northeastward flow of the Tibetan Plateau. High-magnitude earthquakes are associated with depth changes in the CPD and areas with a shallow CPD. The frequent seismic activity in the NETP can be attributed to the northeastward flow of the Tibetan Plateau caused by a deep heat flux. The results can be used as a reference for the prediction of strong regional earthquakes. Full article
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22 pages, 3216 KB  
Article
Effect of Pseudomonas protegens EMM-1 Against Rhizopus oryzae in Interactions with Mexican Autochthonous Red Maize
by Bruce Manuel Morales-Barron, Violeta Larios-Serrato, Yolanda Elizabeth Morales-García, Verónica Quintero-Hernández, Paulina Estrada-de los Santos and Jesús Muñoz-Rojas
Life 2025, 15(4), 554; https://doi.org/10.3390/life15040554 - 28 Mar 2025
Viewed by 1572
Abstract
In the present study, the strain Rhizopus oryzae EMM was isolated from germinated autochthonous red maize seeds, which were harvested in a region of San Diego-Buenavista, Papalotla, Tlaxcala, Mexico, where cobs with fungal infections have been observed. This fungal strain caused wilting in [...] Read more.
In the present study, the strain Rhizopus oryzae EMM was isolated from germinated autochthonous red maize seeds, which were harvested in a region of San Diego-Buenavista, Papalotla, Tlaxcala, Mexico, where cobs with fungal infections have been observed. This fungal strain caused wilting in the maize seedlings. Pseudomonas protegens EMM-1 was tested for its ability to inhibit R. oryzae EMM, both in culture media and in association with maize plantlets. P. protegens EMM-1 inhibited the growth of R. oryzae EMM under all culture media conditions explored. The ability of P. protegens EMM-1 to inhibit the growth of R. oryzae EMM associated with plants was evaluated in both a hydroponic system and in vermiculite. In both systems, P. protegens EMM-1 strongly inhibited the growth of R. oryzae EMM. The dry weight of root plants infected with R. oryzae EMM and inoculated with P. protegens EMM-1 increased to 0.43 g, while that of plants infected only with R. oryzae EMM reached just 0.19 g under hydroponic conditions. However, no differences were observed under vermiculite conditions. The dry weight of the aerial region of plants infected with R. oryzae EMM and inoculated with P. protegens EMM-1 was greater than that of plants infected only with R. oryzae EMM, both under hydroponic and vermiculite conditions. These results indicate that P. protegens EMM-1 inhibits the infection caused by R. oryzae EMM, thereby improving plant growth. Moreover, the genome analysis of P. protegens EMM-1 revealed the presence of several genes that potentially encode for antimicrobial compounds, which could strengthen the potential use of P. protegens EMM-1 as a biocontrol agent in maize plants. Full article
(This article belongs to the Special Issue Trends in Microbiology 2025)
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44 pages, 3769 KB  
Review
A Comprehensive Review of PM-Related Studies in Industrial Proximity: Insights from the East Mediterranean Middle East Region
by Marc Fadel, Eliane Farah, Nansi Fakhri, Frédéric Ledoux, Dominique Courcot and Charbel Afif
Sustainability 2024, 16(20), 8739; https://doi.org/10.3390/su16208739 - 10 Oct 2024
Cited by 3 | Viewed by 4715
Abstract
This comprehensive review synthesizes the current knowledge regarding the characteristics of particulate matter (PM) at locations directly impacted by industrial emissions. A particular emphasis was given to the morphology and size of these particles and their chemical characteristics per type of industrial activity. [...] Read more.
This comprehensive review synthesizes the current knowledge regarding the characteristics of particulate matter (PM) at locations directly impacted by industrial emissions. A particular emphasis was given to the morphology and size of these particles and their chemical characteristics per type of industrial activity. The relationship between the exposure to PM from industrial activities and health issues such as cancer, cardiovascular, and respiratory diseases was also discussed, highlighting significant epidemiological findings. Furthermore, this work highlights the source apportionment of PM in these areas as well as available databases for source profiles. The majority of the studies accentuate the ambiguity found in the identification of industrial sources mainly due to the lack of specific tracers and the overlapping between these sources and other natural and anthropogenic ones. The contribution of industrial sources to PM concentrations is generally less than 10%. Moreover, this review gathers studies conducted in the 18 countries of the East Mediterranean-Middle East (EMME) region, focusing on sites under industrial influence. In these studies, PM10 concentrations range from 22 to 423 μg/m3 while PM2.5 levels vary between 12 and 250 μg/m3. While extensive studies have been conducted in Egypt, Iran, and Lebanon, a lack of research in the UAE, Bahrain, Greece, Israel, Palestine, and Yemen highlights regional disparities in environmental health research. The major industrial sources found in the region were oil and gas industries, metallurgical industries, cement plants, petrochemical complexes, and power plants running on gas or heavy fuel oil. Future research in the region should focus on longitudinal studies and a more detailed chemical analysis of PM in the vicinity of industrial areas to enhance the accuracy of current findings and support effective policy making for air pollution control. Full article
(This article belongs to the Section Air, Climate Change and Sustainability)
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23 pages, 13090 KB  
Article
Accurate UAV Small Object Detection Based on HRFPN and EfficentVMamba
by Shixiao Wu, Xingyuan Lu, Chengcheng Guo and Hong Guo
Sensors 2024, 24(15), 4966; https://doi.org/10.3390/s24154966 - 31 Jul 2024
Cited by 9 | Viewed by 3841
Abstract
(1) Background: Small objects in Unmanned Aerial Vehicle (UAV) images are often scattered throughout various regions of the image, such as the corners, and may be blocked by larger objects, as well as susceptible to image noise. Moreover, due to their small size, [...] Read more.
(1) Background: Small objects in Unmanned Aerial Vehicle (UAV) images are often scattered throughout various regions of the image, such as the corners, and may be blocked by larger objects, as well as susceptible to image noise. Moreover, due to their small size, these objects occupy a limited area in the image, resulting in a scarcity of effective features for detection. (2) Methods: To address the detection of small objects in UAV imagery, we introduce a novel algorithm called High-Resolution Feature Pyramid Network Mamba-Based YOLO (HRMamba-YOLO). This algorithm leverages the strengths of a High-Resolution Network (HRNet), EfficientVMamba, and YOLOv8, integrating a Double Spatial Pyramid Pooling (Double SPP) module, an Efficient Mamba Module (EMM), and a Fusion Mamba Module (FMM) to enhance feature extraction and capture contextual information. Additionally, a new Multi-Scale Feature Fusion Network, High-Resolution Feature Pyramid Network (HRFPN), and FMM improved feature interactions and enhanced the performance of small object detection. (3) Results: For the VisDroneDET dataset, the proposed algorithm achieved a 4.4% higher Mean Average Precision (mAP) compared to YOLOv8-m. The experimental results showed that HRMamba achieved a mAP of 37.1%, surpassing YOLOv8-m by 3.8% (Dota1.5 dataset). For the UCAS_AOD dataset and the DIOR dataset, our model had a mAP 1.5% and 0.3% higher than the YOLOv8-m model, respectively. To be fair, all the models were trained without a pre-trained model. (4) Conclusions: This study not only highlights the exceptional performance and efficiency of HRMamba-YOLO in small object detection tasks but also provides innovative solutions and valuable insights for future research. Full article
(This article belongs to the Section Sensing and Imaging)
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8 pages, 1305 KB  
Brief Report
Bite Me: Bark Stripping Showed Negligible Effect on Volume Growth of Norway Spruce in Latvia
by Agnese Anta Liepiņa, Sabīne Ieviņa, Endijs Bāders, Gundega Done, Roberts Matisons, Ieva Jaunslaviete, Beate Bērziņa and Āris Jansons
Plants 2024, 13(15), 2014; https://doi.org/10.3390/plants13152014 - 23 Jul 2024
Viewed by 1125
Abstract
Over the past few decades, increasing populations of cervid species in the Baltic region have reduced the quality and vitality of cultivated Norway spruce (Picea abies (L.) Karst.) stands. This study evaluated the effect of bark stripping on the volume growth of [...] Read more.
Over the past few decades, increasing populations of cervid species in the Baltic region have reduced the quality and vitality of cultivated Norway spruce (Picea abies (L.) Karst.) stands. This study evaluated the effect of bark stripping on the volume growth of spruce trees in Latvia. Data collection took place in two forest stands. In each stand, 20 Norway spruce trees were sampled, 10 with visible bark damage scars and 10 control trees. Stem discs were collected from control trees at specified heights (0 m, 0.5 m, 1 m, 1.3 m, and 2 m, and then at one-metre intervals up to the top) and from damaged trees at additional specific points relative to the damage. Each disc was sanded and scanned; tree ring widths were measured in 16 radial directions using WinDendro 2012a software. Annual volume growth reconstruction was performed for each tree. Changes in relative volume growth were analysed in interaction with scar parameters, tree type (damaged/control), and pre-damage volume using linear regression models. The significance of parameter interactions was assessed using analysis of variance (ANOVA). Pairwise comparisons of estimated marginal means (EMMs) were conducted using Tukey’s HSD post hoc test. No significant effect of bark stripping on the total stem volume increment was detected. However, the length of bark stripping scars had a significant impact on relative volume growth in the lower parts of the stems. These findings underscore the importance of further research examining a broader spectrum of cervid damage intensity and the effects of repeated damage on tree survival and growth. Full article
(This article belongs to the Section Plant Ecology)
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19 pages, 554 KB  
Article
A Simplified Method for the Evaluation of Floating-Body Motion Responses over a Sloping Bottom
by Xiaolei Liu, Kun Gu, Zhijia Qian, Sheng Ding, Kan Wang, Hao Wang and Chen Sun
J. Mar. Sci. Eng. 2024, 12(5), 756; https://doi.org/10.3390/jmse12050756 - 30 Apr 2024
Cited by 2 | Viewed by 1818
Abstract
Recently, many floating renewable energy platforms have been deployed in coastal regions, where sloping bottoms are an important factor when evaluating their safety. In this article, a simplified method coupling an eigenfunction matching method (EMM) and a finite-depth Green’s function (FDGF) is developed [...] Read more.
Recently, many floating renewable energy platforms have been deployed in coastal regions, where sloping bottoms are an important factor when evaluating their safety. In this article, a simplified method coupling an eigenfunction matching method (EMM) and a finite-depth Green’s function (FDGF) is developed to evaluate floating-body motion responses over a sloping bottom for which bathymetry is homogeneous in the longshore direction. We propose an extended EMM to create an incident wave model over the sloping bottom, thereby obtaining the Froude–Krylov (F–K) force and Neumann data on the wet surfaces of the floating body for the diffraction problem. An equivalent depth is introduced to account for the interaction between the sloping bottom and floating bodies when dealing with the diffraction and radiation problems. The accuracy of the present method is validated through a comprehensive comparison with numerical and/or experiment results for a liquefied natural gas (LNG) ship and a floating hemisphere from the literature. Generally, the present, simplified method can give predictions with sufficient accuracy. Full article
(This article belongs to the Section Ocean Engineering)
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6 pages, 1454 KB  
Proceeding Paper
Modelling the Urban Thermal Environment through the Combined Use of WRF and the Local Climate Zones Approach: Case Study for Nicosia
by Konstantina Koutroumanou-Kontosi, Constantinos Cartalis, Panos Hadjinicolaou, Katiana Constantinidou and Ilias Agathangelidis
Environ. Sci. Proc. 2023, 26(1), 53; https://doi.org/10.3390/environsciproc2023026053 - 25 Aug 2023
Cited by 1 | Viewed by 1785
Abstract
The Eastern Mediterranean and Middle East (EMME) region is an exceptionally thermally vulnerable area, projected to suffer from frequent and severe heatwaves in the coming decades. To assess the impacts of climate change on the urban thermal environment in cities, high-resolution numerical simulations [...] Read more.
The Eastern Mediterranean and Middle East (EMME) region is an exceptionally thermally vulnerable area, projected to suffer from frequent and severe heatwaves in the coming decades. To assess the impacts of climate change on the urban thermal environment in cities, high-resolution numerical simulations are crucial. In this study, the Weather Research and Forecasting (WRF) model is tested in order to progressively downscale the regional scale (12 km) to the local scale (1 km) and thus derive high-resolution data for the 2 m air temperature over Nicosia. Two different simulations were conducted, driven by the ERA5 re-analysis, over a two-month summer period, namely from 15 June to 15 August, 2021, to compare two different urban canopy schemes. The first simulation concerns the implementation of the bulk scheme using the default land cover of MODIS. In the second, the WRF model was coupled with the Single Layer Urban Canopy Model for a better representation of the urban characteristics of Nicosia, and detailed information on the urban form was inserted into the model via the creation of the Local Climate Zones classification scheme. A comparison between the two different physical schemes as well as an evaluation of the simulation results with observation data are performed. Full article
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12 pages, 2152 KB  
Article
N-Source Determines Barley Productivity, Nutrient Accumulation, and Grain Quality in Cyprus Rainfed Agricultural Systems
by Michalis Omirou, Dionysia Fasoula, Marinos Stylianou, Antonis A. Zorpas and Ioannis M. Ioannides
Int. J. Environ. Res. Public Health 2023, 20(5), 3943; https://doi.org/10.3390/ijerph20053943 - 23 Feb 2023
Cited by 7 | Viewed by 2291
Abstract
The Eastern Mediterranean and Middle East (EMME) region is already experiencing the negative effects of increased temperatures and the increase in prolonged drought periods. The use of organic fertilization could be a valuable tool to meet the main challenges of climate change and [...] Read more.
The Eastern Mediterranean and Middle East (EMME) region is already experiencing the negative effects of increased temperatures and the increase in prolonged drought periods. The use of organic fertilization could be a valuable tool to meet the main challenges of climate change and maintain the productivity, quality, and sustainability of rainfed agricultural ecosystems. In the current study, we compare the effect of manure, compost, and chemical fertilization (NH4NO3) on barley grain and straw yield in a field study for three consecutive growing seasons. The hypothesis that the barley productivity, nutrient accumulation, and grain quality remain similar among the different nutrient management strategies was tested. The results showed that both growing season and type of nutrient source significantly affected barley grain and straw yield (F6,96 = 13.57, p < 0.01). The lowest productivity was noticed in the non-fertilized plots while chemical and organic fertilization exhibited similar grain yield, ranging from 2 to 3.4 t/ha throughout the growing seasons. For straw, the use of compost had no effect on the yield in any of the growing seasons examined. The use of manure and compost had a significant effect on grain macro- and micronutrient content but this was highly related to growing season. Principal component analysis (PCA) clearly demonstrated the discrimination of the different type of fertilization on barley performance during the course of the study, while the application of compost was highly associated with an increase in micronutrients in grain samples. Furthermore, structural equational modeling (SEM) showed that both chemical and organic fertilization had a direct positive effect on macro- (r = 0.44, p < 0.01) and micronutrient (r = 0.88, p < 0.01) content of barley grain and a positive indirect effect on barley productivity through N accumulation in grain (β = 0.15, p = 0.007). The current study showed that barley grain and straw yield was similar between manure and NH4NO3 treatments, while compost exhibited a residual positive effect causing an increase in grain yield during the growing season. The results highlight that N fertilization under rainfed conditions is beneficial to barley productivity through its indirect effects on N accumulation in grain and straw, while it improves grain quality through the increased accumulation of micronutrients. Full article
(This article belongs to the Special Issue Carbon Footprint and the Environment)
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26 pages, 13008 KB  
Article
MAGNet: A Camouflaged Object Detection Network Simulating the Observation Effect of a Magnifier
by Xinhao Jiang, Wei Cai, Zhili Zhang, Bo Jiang, Zhiyong Yang and Xin Wang
Entropy 2022, 24(12), 1804; https://doi.org/10.3390/e24121804 - 9 Dec 2022
Cited by 14 | Viewed by 5628
Abstract
In recent years, protecting important objects by simulating animal camouflage has been widely employed in many fields. Therefore, camouflaged object detection (COD) technology has emerged. COD is more difficult to achieve than traditional object detection techniques due to the high degree of fusion [...] Read more.
In recent years, protecting important objects by simulating animal camouflage has been widely employed in many fields. Therefore, camouflaged object detection (COD) technology has emerged. COD is more difficult to achieve than traditional object detection techniques due to the high degree of fusion of objects camouflaged with the background. In this paper, we strive to more accurately and efficiently identify camouflaged objects. Inspired by the use of magnifiers to search for hidden objects in pictures, we propose a COD network that simulates the observation effect of a magnifier called the MAGnifier Network (MAGNet). Specifically, our MAGNet contains two parallel modules: the ergodic magnification module (EMM) and the attention focus module (AFM). The EMM is designed to mimic the process of a magnifier enlarging an image, and AFM is used to simulate the observation process in which human attention is highly focused on a particular region. The two sets of output camouflaged object maps were merged to simulate the observation of an object by a magnifier. In addition, a weighted key point area perception loss function, which is more applicable to COD, was designed based on two modules to give greater attention to the camouflaged object. Extensive experiments demonstrate that compared with 19 cutting-edge detection models, MAGNet can achieve the best comprehensive effect on eight evaluation metrics in the public COD dataset. Additionally, compared to other COD methods, MAGNet has lower computational complexity and faster segmentation. We also validated the model’s generalization ability on a military camouflaged object dataset constructed in-house. Finally, we experimentally explored some extended applications of COD. Full article
(This article belongs to the Topic Applications in Image Analysis and Pattern Recognition)
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25 pages, 2667 KB  
Article
Assessment of Seasonal Energy Efficiency Strategies of a Double Skin Façade in a Monsoon Climate Region
by Taehoon Hong, Jimin Kim, Juyoung Lee, Choongwan Koo and Hyo Seon Park
Energies 2013, 6(9), 4352-4376; https://doi.org/10.3390/en6094352 - 23 Aug 2013
Cited by 40 | Viewed by 11366
Abstract
As climate change and global warming have become two of the most significant environmental issues today, the double-skin façade (DSF) is getting considerable attention as an energy-efficient passive design. This study is aimed at assessing the seasonal energy efficiency strategies of a DSF [...] Read more.
As climate change and global warming have become two of the most significant environmental issues today, the double-skin façade (DSF) is getting considerable attention as an energy-efficient passive design. This study is aimed at assessing the seasonal energy efficiency strategies of a DSF targeting library facilities in the climate region with hot summers and cold winters. Toward this end, this study was conducted in four steps: (i) establishment of seasonal energy-efficient strategies; (ii) application of seasonal energy-efficient strategies; (iii) analysis of energy saving effect by season; and (iv) life cycle cost and life cycle CO2 analyses for selecting an optimal DSF. Results show that a shaft box DSF energy model (EMS) #2, which applied winter strategies, was optimal with an energy saving rate of 4.13%, while a multi-story DSF energy model (EMM) #5, which applied summer strategies, was optimal with an energy saving rate of 12.67%. In terms of savings to investment ratio (SIR40) and breakeven point (BEP40), the multi-story DSF (3.20; 9 years) was superior. The results of this study can be used for (i) seasonal energy efficiency strategies of a DSF in East Asian monsoon climates, and (ii) as a guideline for the application of a DSF both in existing and new buildings. Full article
(This article belongs to the Special Issue Energy Efficient Building Design 2013)
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