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Keywords = Dermestes

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13 pages, 396 KB  
Article
Potential for Using Beetles (Coleoptera: Dermestidae) as Model Organisms to Determine Nutrient Bioavailability for Companion Animal Foods: A Pilot Study
by Mollie Toth, Charles G. Aldrich and Thomas W. Phillips
Animals 2025, 15(17), 2630; https://doi.org/10.3390/ani15172630 - 8 Sep 2025
Viewed by 915
Abstract
Pet owners have become more aware of how their pet’s nutrition can influence the pet’s life expectancy, wellness, and energy. Evaluating the new ingredients claiming better pet health for nutrient contributions requires significant investment and targeted animal commitment while current research aims to [...] Read more.
Pet owners have become more aware of how their pet’s nutrition can influence the pet’s life expectancy, wellness, and energy. Evaluating the new ingredients claiming better pet health for nutrient contributions requires significant investment and targeted animal commitment while current research aims to find alternative approaches. Research on pet foods in universities is overseen by the Institutional Animal Care and Use Committee, which has restrictions on the use of vertebrates in testing but places no restrictions on the use of insects. The research reported here studied three species of beetles (Coleoptera: Dermestidae), Trogoderma variabile (Ballion), Trogoderma inclusum (LeConte), and Dermestes maculatus (DeGeer) as potential model organisms to evaluate pet food nutrient bioavailability. The larvae of all three species were fed a balanced pet food diet under various laboratory conditions to determine their protein efficiency ratios (PERs) over a 144 h period. Results suggested that D. maculatus larvae reared at 27 °C, 60–65% R.H. (relative humidity), and a photoperiod of 16:8 L:D were the most efficient at converting ingested protein into weight gain with an average protein efficiency ratio (PER) of 1.439. Further experiments were conducted using only D. maculatus larvae with shorter time windows of 48 h and 24 h. The 24 h feeding time was successful and yielded an average PER of 2.476. These findings suggest that D. maculatus larvae may be a useful model organism for pet food bioavailability studies. Full article
(This article belongs to the Section Animal Nutrition)
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12 pages, 826 KB  
Article
Identification of Necrophagous Beetles (Coleoptera) Using Low-Resolution Real-Time PCR in the Buffer Zone of Kampinos National Park
by Tadeusz Malewski, Katarzyna Leszczyńska, Katarzyna Daria Borzuchowska, Maciej Sierakowski, Tomasz Oszako and Justyna Anna Nowakowska
Insects 2025, 16(2), 215; https://doi.org/10.3390/insects16020215 - 15 Feb 2025
Cited by 1 | Viewed by 2255
Abstract
Forensic entomology is concerned with the study of arthropods that assist in criminal investigations. Among them, necrophagous beetles (Coleoptera) play a crucial role as they are found in all stages of decomposition of corpses, which makes them valuable for forensic analysis. Accurate species [...] Read more.
Forensic entomology is concerned with the study of arthropods that assist in criminal investigations. Among them, necrophagous beetles (Coleoptera) play a crucial role as they are found in all stages of decomposition of corpses, which makes them valuable for forensic analysis. Accurate species identification is crucial for the effective use of entomological evidence. Efficient, cost-effective, and rapid methods are essential for this purpose. In the present study, we developed a novel assay that enables simple, inexpensive, and rapid identification of necrophagous beetle species. Using two primer sets targeting the cytochrome c oxidase 1 (COI) gene and analyzing the resulting SYBR Green I melting curves, we were able to identify fourteen beetle species: Aleochara curtula (Goeze, 1777), Anoplotrupes stercorosus (Scriba, 1791), Creophilus maxillosus (Linnaeus, 1758), Dermestes undulatus (Brahm, 1790), Hister unicolor (Linnaeus, 1758), Margarinotus brunneus (Fabricius, 1775), Necrodes littoralis (Linnaeus, 1758), Nicrophorus investigator (Zetterstedt, 1824), Ontholestes murinus (Linnaeus, 1758), Oiceoptoma thoracicum (Linnaeus, 1758), Philonthus cognatus (Stephens, 1832), Saprinus planiusculus (Motschulsky, 1849), Silpha tristis (Illiger, 1798), and Thanatophilus rugosus (Linnaeus, 1758). Data on the melting temperature of the amplicon can be obtained within 60–90 min after DNA extraction. This study expands the understanding of the species composition of necrophagous Coleoptera, which is crucial for forensic entomology methods based on insect succession. Furthermore, it provides the first comprehensive data on necrophagous beetles in the vicinity of Kampinos National Park. Full article
(This article belongs to the Special Issue Forensic Entomology: From Basic Research to Practical Applications)
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14 pages, 2800 KB  
Article
Envenomation with Snake Venoms as a Cause of Death: A Forensic Investigation of the Decomposition Stages and the Impact on Differential Succession Pattern of Carcass-Attracted Coleopteran Beetles
by Abdelwahab Khalil, Abeer M. Salem, El-Sayed H. Shaurub, Ashraf M. Ahmed, Areej A. Al-Khalaf and Mahmoud M. Zidan
Insects 2024, 15(11), 902; https://doi.org/10.3390/insects15110902 - 18 Nov 2024
Cited by 2 | Viewed by 2031
Abstract
Background: Coleoptera is the second-most significant insect group associated with decomposing carcasses, yet its role in the decomposition process and postmortem colonization following envenomation is poorly understood. Purpose of the Study: This study aimed to investigate the effects of the venoms from Cerastes [...] Read more.
Background: Coleoptera is the second-most significant insect group associated with decomposing carcasses, yet its role in the decomposition process and postmortem colonization following envenomation is poorly understood. Purpose of the Study: This study aimed to investigate the effects of the venoms from Cerastes cerastes and Naja haje on the decomposition of rabbit carcasses while evaluating the main beetle taxa attracted to these decaying remains. Methods: Three groups of rabbits, each with five individuals, were utilized. The first group was injected with the venom of Cerastes cerastes, the second with Naja haje venom, and the control group received 0.85% physiological saline before euthanasia with CO2. Results: Four decomposition stages (fresh, bloating, decay, and dry) with durations varying based on venom type and carcass condition were observed. A total of 647 individual beetles of six species (Necrobia rufipes, Attagenus sp., Dermestes frischii, D. maculatus, Bledius sp., and Apentanodes sp.) belonging to four families (Cleridae, Dermestidae, Staphylinidae, and Tenebrionidae) were collected and identified. D. maculatus was the most abundant species. Fewer beetles were attracted to carcasses envenomed with N. haje compared to the other groups. Conclusions: Envenomation by snake venom influences the attraction and succession rate of necrophilous coleopterans to carcasses, which is important for forensic investigations. Full article
(This article belongs to the Section Role of Insects in Human Society)
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13 pages, 4877 KB  
Article
Dermestes (s.str.) haemorrhoidalis (Coleoptera: Dermestidae)—The Most Frequent Species on Mummified Human Corpses in Indoor Conditions? Three Cases from Southwestern Poland
by Marcin Kadej, Łukasz Szleszkowski, Agata Thannhäuser and Tomasz Jurek
Insects 2023, 14(1), 23; https://doi.org/10.3390/insects14010023 - 25 Dec 2022
Cited by 9 | Viewed by 7699
Abstract
Beetles of the family Dermestidae, especially of the genus Dermestes Linnaeus, 1758, are often identified on corpses in late stages of decomposition. They usually feed on remains devoid of organs and soft tissues or when the corpses undergo mummification. In Europe, eight species [...] Read more.
Beetles of the family Dermestidae, especially of the genus Dermestes Linnaeus, 1758, are often identified on corpses in late stages of decomposition. They usually feed on remains devoid of organs and soft tissues or when the corpses undergo mummification. In Europe, eight species from two subgenera Dermestes and Dermestinus Zhantiev, 1967, have so far been identified on human corpses. Despite the relatively frequent presence of Dermestes sp. in experimental studies conducted in Poland, no reports concerning Dermestes directly collected from human corpses have been published to date. This article again describes observations of Dermestidae collected from human corpses found in indoor conditions in Wrocław, the capital of the Dolnośląskie Voivodeship. For the second time, there is evidence of the presence of Dermestes (s.str.) haemorrhoidalis on human corpses—a species considered to be relatively rare, as evidenced by faunistic data published from Poland, as well as the results of ongoing experiments of forensic interest. Full article
(This article belongs to the Special Issue Advanced Research on Forensic Entomology)
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11 pages, 11616 KB  
Article
Experimental Evidence of Bone Lesions Due to Larder Beetle Dermestes maculatus (Coleoptera: Dermestidae)
by Damien Charabidzé, Vincent Lavieille and Thomas Colard
Biology 2022, 11(9), 1321; https://doi.org/10.3390/biology11091321 - 6 Sep 2022
Cited by 14 | Viewed by 3952
Abstract
Dermestid beetles (Coleoptera: Dermestidae) are necrophagous insects feeding on mummified carcasses. After six to seven molts, the larvae stop feeding and dig pupation chambers to hide and safely evolve into adults. Such pupation chambers have already been observed on archaeological mammals’ bones, but [...] Read more.
Dermestid beetles (Coleoptera: Dermestidae) are necrophagous insects feeding on mummified carcasses. After six to seven molts, the larvae stop feeding and dig pupation chambers to hide and safely evolve into adults. Such pupation chambers have already been observed on archaeological mammals’ bones, but the attribution and interpretation of these osteological lesions lack experimental evidence in a forensic context. To observe whether dermestid larvae dig pupation chambers in human bones, 20 or 40 Dermestes maculatus (De Geer, 1774) larvae were placed in a dermestarium with different types of bones varying in species (Bos taurus or human), age (adult or immature), and preservation method (fresh or dry). Our results show that dermestid larvae caused multiple lesions, including larval mandible traces on cortical bone, cortical perforations, drilling of pupation chambers, destruction of the trabecular network, and the perforation of cartilage. Bone destruction was mainly observed on aged dry bones, while fresh bones only exhibited soft tissue and superficial cartilage lesions. According to these results, pupation chambers could indicate the simultaneous presence of several corpses at different decomposition stages, or the addition of new corpses while others were already skeletonized. These conclusions are particularly important in the case of mass graves, where chronology is sometimes difficult to establish. Full article
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10 pages, 6179 KB  
Article
Behavioral and Electrophysiological Responses of the Fringed Larder Beetle Dermestes frischii to the Smell of a Cadaver at Different Decomposition Stages
by Clément Martin, Damien Minchilli, Frédéric Francis and François Verheggen
Insects 2020, 11(4), 238; https://doi.org/10.3390/insects11040238 - 10 Apr 2020
Cited by 10 | Viewed by 4531
Abstract
A cadaver is colonized by a wide diversity of necrophagous insects. It is well documented that Dipterans are attracted by the volatile organic compounds (VOCs) released by a corpse during the first minutes following death. Coleopterans are known to be attracted by highly [...] Read more.
A cadaver is colonized by a wide diversity of necrophagous insects. It is well documented that Dipterans are attracted by the volatile organic compounds (VOCs) released by a corpse during the first minutes following death. Coleopterans are known to be attracted by highly decomposed cadavers, but have received less attention regarding the olfaction-based mechanisms underlying these interactions. In the present study, we impregnated gauzes with VOCs collected from each decomposition stage of dead rats: fresh, bloated, active, and advanced decay. We collected the VOCs released by the gauze and confirmed what was previously know from the literature: the decomposition stages are associated with contrasting chemical profiles. We exposed Dermestes frischii Kugelann (Coleoptera: Dermestidae) male and female antennae to the same gauzes and found that stronger electrical responses were recorded when using the smell of the advanced decay stage. Finally, we performed two choices behavioral assays. Females showed no preference for the four decomposition stages, while males were attracted by the smell associated with active and advanced decay stages. These results suggest that specific VOCs released by a decaying body guide necrophagous coleopterans to their feeding site. Whether D. frischii males release pheromones to attract females remains to be tested. Full article
(This article belongs to the Special Issue Forensic Entomology: Beyond the Bounds of Possibility)
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6 pages, 203 KB  
Article
Cool Down–Warm Up: Differential Responses of Stored Product Insects after Gradual Temperature Changes
by Christos G. Athanassiou and Frank H. Arthur
Insects 2020, 11(3), 158; https://doi.org/10.3390/insects11030158 - 1 Mar 2020
Cited by 11 | Viewed by 2943
Abstract
Insect survival after exposure to 0 °C for 7 days was examined in laboratory bioassays for control of adults of six major stored-product beetle species, Oryzaephilus surinamensis (L.), the sawtoothed grain beetle, Cryptolestes ferrugineus, (Stephens), the rusty grain beetle, Dermestes maculatus DeGeer, [...] Read more.
Insect survival after exposure to 0 °C for 7 days was examined in laboratory bioassays for control of adults of six major stored-product beetle species, Oryzaephilus surinamensis (L.), the sawtoothed grain beetle, Cryptolestes ferrugineus, (Stephens), the rusty grain beetle, Dermestes maculatus DeGeer, the hide beetle, Sitophilus oryzae (L.), the rice weevil, Tribolium castaneum (Herbst), the red flour beetle, and T. confusum Jacquelin DuVal, the confused flour beetle In this test there were four different acclimation treatments, insects that had been subjected to a pre-acclimation period to 0 °C, a post-acclimation period, both a pre and post-acclimation period, and adults that were not acclimated. Insect survival for all species except S. oryzae was not affected by the exposure to 0 °C, regardless of the acclimation scenario. In contrast, exposure to 0 °C drastically reduced survival of S. oryzae. Moreover, adults that were exposed to the post-acclimation only and un-acclimated adults had lower survival rates than those that had either exposure to pre-acclimation, or to both pre- and post-acclimation. Results of this experiment show that acclimation played a limited role in adult survival of five of the six tested species, and that exposure of adults to 0 °C for 7 d had no effect in survival of these species as well. Full article
13 pages, 105 KB  
Review
Insect Pests and Integrated Pest Management in Museums, Libraries and Historic Buildings
by Pascal Querner
Insects 2015, 6(2), 595-607; https://doi.org/10.3390/insects6020595 - 16 Jun 2015
Cited by 93 | Viewed by 16143
Abstract
Insect pests are responsible for substantial damage to museum objects, historic books and in buildings like palaces or historic houses. Different wood boring beetles (Anobium punctatum, Hylotrupes bajulus, Lyctus sp. or introduced species), the biscuit beetle (Stegobium paniceum), [...] Read more.
Insect pests are responsible for substantial damage to museum objects, historic books and in buildings like palaces or historic houses. Different wood boring beetles (Anobium punctatum, Hylotrupes bajulus, Lyctus sp. or introduced species), the biscuit beetle (Stegobium paniceum), the cigarette beetle (Lasioderma serricorne), different Dermestides (Attagenus sp., Anthrenus sp., Dermestes sp., Trogoderma sp.), moths like the webbing clothes moth (Tineola bisselliella), Silverfish (Lepisma saccharina) and booklice (Psocoptera) can damage materials, objects or building parts. They are the most common pests found in collections in central Europe, but most of them are distributed all over the world. In tropical countries, termites, cockroaches and other insect pests are also found and result in even higher damage of wood and paper or are a commune annoyance in buildings. In this short review, an introduction to Integrated Pest Management (IPM) in museums is given, the most valuable collections, preventive measures, monitoring in museums, staff responsible for the IPM and chemical free treatment methods are described. In the second part of the paper, the most important insect pests occurring in museums, archives, libraries and historic buildings in central Europe are discussed with a description of the materials and object types that are mostly infested and damaged. Some information on their phenology and biology are highlighted as they can be used in the IPM concept against them. Full article
(This article belongs to the Special Issue Integrated Pest Management)
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