Search Results (3)

To demonstrate causation or/and assess pathogenic mechanisms of environment-induced autoimmunity, various animal models that mimic the characteristics of the human autoimmune diseases need to be developed. Experimental studies in mice reveal the genetic factors that contribute to autoimmune diseases. Here, the immune response of two mouse strains congenic for non-H-2 genes, A.TL (H-2^tl) and A.SW (H-2^s), was evaluated after 15 weeks’ exposure to gold aurothiomalate (AuTM). AuTM-treated A.TL mice showed anti-nuclear antibodies (ANA) with homogenous and/or fine speckled staining patterns and serum autoantibodies to ds-DNA, chromatin, histones, and ribonucleoproteins (RNP). Female A.TL mice showed a stronger immune response than males, as well as an increase of B cells in their spleen after 15 weeks of gold exposure. A.SW exposed for AuTM showed the induction of anti-nucleolar antibodies (ANoA) with a clumpy staining pattern, as well as an increase in splenic B and T cells. The serum autoantibodies levels in A.SW mice were limited compared to those of A.TL mice. Overall, A.TL presents a stronger immune response after gold exposure than A.SW. The immune response developed in A.TL presents similarities with the clinical manifestations in human autoimmune diseases. Thus, gold-exposed A.TL could constitute a potential experimental mouse model for the study of autoimmunity. Full article

The Anoa is a wild animal endemic to Sulawesi that looks like a small cow. Anoa are categorized as vulnerable to extinction on the IUCN red list. There are two species of Anoa, namely Lowland Anoa (Bubalus depressicornis) and Mountain Anoa (Bubalus quarlesi). In this study, a comparison of potential habitat models for Anoa species was conducted using Machine Learning algorithms with the Maximum Entropy (MaxEnt) and Random Forest (RF) methods. This modeling uses eight environmental variables. Where based on the results of Bubalus quarlesi potential habitat modeling, the RF 75:25 model is the best algorithm with the highest variable contribution, namely humidity of 82.444% and a potential area of 5% of Sulawesi Island, with an Area Under Curve (AUC) of 0.987. Meanwhile, the best Bubalus depressicornis habitat potential model is the RF 70:30 algorithm, with the highest variable contribution, namely population of 88.891% and potential area of 36% of Sulawesi Island, with AUC 0.967. This indicates that Anoa extinction is very sensitive to the presence of humidity and human population levels. Full article

Mesenchymalstem cell (MSC)-based therapy is being increasingly explored in preclinical and clinical studies as a regenerative method for treating osteoarthritis (OA). However, the use of primary MSCs is hampered by a number of limitations, including donor heterogeneity and inconsistent cell quality. Here, we tested the therapeutic potential of embryonic stem cell-derived MSCs (ES-MSCs) in anOA rat model. ES-MSCs were generated and identified by morphology, trilineage differentiation and flow cytometry. Sprague Dawley rats were treated with either a single dose (10⁶ cells/rat) of ES-MSCs or with three doses spaced one week apart for each dose, starting at four weeks after anterior cruciate ligament transectionto induce OA. Cartilage quality was evaluated at 6 and 10 weeks after treatment with behavioral analysis, macroscopic examination, and histology. At sixweeks after treatment, the groups treated with both single and repeated doses of ES-MSCs had significantly better modified Mankin scores and International Cartilage Repair Society (ICRS) macroscopic scores in the femoral condyle compared to the control group. At 10 weeks after treatment, the repeated doses group had a significantly better ICRS macroscopic scores in the femoral condyle compared to the single dose and control groups. Histological analysis also showed more proteoglycan and less cartilage loss, along with lower Mankin scores in the repeated doses group. In conclusion, treatment with multiple injections of ES-MSCs can ameliorate OA in a rat model. TheES-MSCs have potential to be considered as a regenerative therapy for OA, and can provide an infinite cellular source. Full article