Search Results (9)

Excessive land application of poultry litter (PL) may lead to surface runoff of nitrogen (N) and phosphorus (P), which cause eutrophication, fish death, and water pollution that ultimately have negative effects on humans and animals. Increases in poultry production in the Delmarva Peninsula underscore the need for more efficient, cost-effective, and sustainable disposal technologies for processing PL instead of direct land application. The pyrolysis conversion process can potentially produce nutrient-rich poultry litter biochar (PLB), while the pyrolysis process can change the N and P to a more stable component, thus reducing its runoff. Pyrolysis also kills off any microorganisms that would otherwise trigger negative environmental health effects. This study is to apply an integrated method and investigate the effect of pyrolysis temperature (300 °C, 500 °C), poultry litter source (different feedstock composition), and bedding material mixture (10% pine shavings) on PLB qualities and quantities. Proximate and ultimate analysis showed PL sources and bedding material addition influenced the physicochemical properties of feedstock. The SEM and BET surface results indicate that pyrolysis temperature had a significant effect on changing the PLB morphology and structure, as well as the pH value (7.78 at 300 °C vs. 8.78 at 500 °C), extractable phosphorus (P) (18.73 ppm at 300 °C vs. 11.72 ppm at 500 °C), sulfur (S) (363 ppm at 300 °C vs. 344 ppm at 500 °C), and production yield of PLBs (47.65% at 300 °C vs. 60.62% at 500 °C). The results further suggest that adding a bedding material mixture (10% pine shavings) to PLs improved qualities by reducing the content of extractable P and S, as well as pH values of PLBs. This study also found the increment in both the pore volume and the area of Bethel Farm was higher than that of Sun Farm. Characterization and investigation of qualities and quantities of PLB using the integrated framework suggest that PL from Bethel Farm could produce better-quality PLB at a higher pyrolysis temperature and bedding material mixture to control N and P runoff problems. Full article

In previous work, we showed that cancer cells do not depend on glycolysis for ATP production, but they do on fatty acid oxidation. However, we found some cancer cells induced cell death after glucose deprivation along with a decrease of ATP production. We investigated the different response of glucose deprivation with two types of cancer cells including glucose insensitive cancer cells (GIC) which do not change ATP levels, and glucose sensitive cancer cells (GSC) which decrease ATP production in 24 h. Glucose deprivation-induced cell death in GSC by more than twofold after 12 h and by up to tenfold after 24 h accompanied by decreased ATP production to compare to the control (cultured in glucose). Glucose deprivation decreased the levels of metabolic intermediates of the pentose phosphate pathway (PPP) and the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) in both GSC and GIC. However, glucose deprivation increased reactive oxygen species (ROS) only in GSC, suggesting that GIC have a higher tolerance for decreased NADPH than GSC. The twofold higher ratio of reduced/oxidized glutathione (GSH/GSSG) in GIS than in GSC correlates closely with the twofold lower ROS levels under glucose starvation conditions. Treatment with N-acetylcysteine (NAC) as a precursor to the biologic antioxidant glutathione restored ATP production by 70% and reversed cell death caused by glucose deprivation in GSC. The present findings suggest that glucose deprivation-induced cancer cell death is not caused by decreased ATP levels, but rather triggered by a failure of ROS regulation by the antioxidant system. Conclusion is clear that glucose deprivation-induced cell death is independent from ATP depletion-induced cell death. Full article

Increasing production of poultry litter, and its associated problems, stimulates the need for generating useful energy in an environmentally friendly and efficient energy system, such as the use of shell-and-tube heat exchangers (STHE) in a fluidized-bed combustion (FBC) system. A holistic approach which involves the integration of the First Law of Thermodynamics (FLT) and Second Law of Thermodynamics (SLT) is required for conducting effective assessment of an energy system. In this study, the STHE designed by the CAESECT research group, which was integrated into the lab-scale FBC, was investigated to determine the maximum available work performed by the system and account for the exergy loss due to irreversibility. The effects of varying operating parameters and configuration of the space heaters connected to the STHE for space heating purposes were investigated in order to improve the thermal efficiency of the poultry litter-to-energy conversion process. Exergy and energy analysis performed on the STHE using flue gas and water media showed higher efficiency (75–92%) obtained via energy analysis, but much lower efficiency (12–25%) was obtained when the ambient conditions were factored into the exergy analysis, thus indicating huge exergy loss to the surroundings. From the obtained experimental data coupled with the simulation on parallel arrangement of air heaters, it was observed that exergy loss increased with increasing flue gas flow rate from 46.8–57.6 kg/h and with increasing ambient temperature from 8.8 °C to 25 °C. To lower the cost of STHE during final design, a larger temperature difference between the hot and cold flue gas is needed throughout the exchanger, which further increases the exergetic loss while maintaining an energy balance. In addition, this study also found the optimal conditions to reduce exergy loss and improve energy efficiency of the designed STHE. This study shows the possibility to evaluate energy systems using integration of exergy and energy analysis. Full article

Foot-and-mouth disease (FMD) is a notifiable contagious disease of cloven-hoofed mammals. A high potency vaccine that stimulates the host immune response is the foremost strategy used to prevent disease persistence in endemic regions. FMD vaccines comprise inactivated virus antigens whose immunogenicity is potentiated by immunogenic adjuvants. Oil-based adjuvants have clear advantages over traditional adjuvant vaccines; however, there is potential to develop novel adjuvants to increase the potency of FMD vaccines. Thus, we aimed to evaluate the efficacy of a novel water-in-oil emulsion, called CAvant^®SOE, as a novel vaccine adjuvant for use with inactivated FMD vaccines. In this study, we found that inactivated A22 Iraq virus plus CAvant^®SOE (iA22 Iraq-CAvant^®SOE) induced effective antigen-specific humoral (IgG, IgG1, and IgG2a) and cell-mediated immune responses (IFN-γ and IL-4) in mice. Immunization of pigs with a single dose of iA22 Iraq-CAvant^®SOE also elicited effective protection, with no detectable clinical symptoms against challenge with heterologous A/SKR/GP/2018 FMDV. Levels of protection are strongly in line with vaccine-induced neutralizing antibody titers. Collectively, these results indicate that CAvant^®SOE-adjuvanted vaccine is a promising candidate for control of FMD in pigs. Full article

The prediction and pre-evaluation of the thermal properties and combustion-related problems (e.g., emissions and ash-related problems) are critical to reducing emissions and improving combustion efficiency during the agricultural crop residues combustion process. This study integrated the higher heating value (HHV) model, specific heat model, and fuel indices as a new systematic approach to characterize the agricultural crop residues. Sixteen linear and non-linear regression models were developed from three main compositions of the ultimate analysis (e.g., C, H, and O) to predict the HHV of the agricultural crop residues. Newly developed HHV models have been validated with lower estimation errors and a higher degree of accuracy than the existing models. The specific heat of flue gas during the combustion process was estimated from the concentrations of C, H, O, S, and ash content under various excess air (EA) ratios and flue gas temperatures. The specific heat of agricultural crop residues was between 1.033 to 1.327 kJ/kg·K, while it was increased by decreasing the EA ratios and elevating the temperature of the flue gas. Combustion-related problems, namely corrosions, PM_1.0 emissions, SO_x, HCl, and ash-related problems were predicted using the fuel indices along with S and Cl concentrations, and ash compositions. Results showed that agricultural crop residues pose a severe corrosion risk and lower ash sintering temperature. This integrated approach can be applied to a wide range of biomass before the actual combustion process which may predict thermal-chemical properties and reduce the potential combustion-related emissions. Full article

In the past few decades, water and air were commonly used as working fluid to evaluate shell and tube heat exchanger (STHE) performance. This study was undertaken to estimate heat transfer coefficients and evaluate performance in the pilot-scale twisted tube-based STHE using the flue gas from biomass co-combustion as working fluid. Theoretical calculation along with experimental results were used to calculate the specific heat of flue gas. A simplified model was then developed from the integration of two heat transfer methods to predict the overall heat transfer coefficient without tedious calculation of individual heat transfer coefficients and fouling factors. Performance including water and trailer temperature, heat load, effectiveness, and overall heat transfer coefficient were jointly investigated under variable operating conditions. Results indicated that the specific heat of flue gas from co-combustion ranging between 1.044 and 1.338 kJ/kg$·$K while specific heat was increased by increasing flue gas temperature and decreasing excess air ratio. The developed mathematical model was validated to have relatively small errors to predict the overall heat transfer coefficient. A flue gas mass flow rate of 61.3–98.8 kg/h, a water flow rate of 13.7–14.1 L/min, and a parallel arrangement of two water-to-air heaters in an empty trailer were found to be optimal conditions for space heating purpose. In addition, a lower poultry litter feeding rate decreased heat loss of flue gas and increased heat gain of water, while a lower water flow rate also provided a lower maximum possible heat transfer rate with a higher actual heat transfer rate to quickly achieve heat equilibrium that ultimately improves the performance. This study demonstrates the possibility of collecting residual heat from the flue gas using the pilot-scale STHE system while outlining a systematic approach and process for evaluating its performance. Full article

Compared with the waste-to-heat and electricity-based hybrid refrigeration system, the innovative lab-scale refrigeration system integrated with the DC and AC cooling units that able to use solar and electricity as energy resources. Previous studies found that temperature control and uniform temperature distribution in refrigeration systems are both critical factors reducing vibrio growth on raw oysters and saving energy consumption. Therefore, this refrigeration system also equipped a specially designed divider and was used to test various air circulation strategies to achieve uniform temperature distribution in six individual compartments. The objective is to investigate and evaluate the effects of air circulation strategies and operating conditions on the cooling performance, including temperature distribution, standard deviation of compartment temperatures, and cooling time using a factorial design method. Results indicated the maximum temperature difference between the compartments was 8.9 ± 2.0 °C, 6.7 ± 2.0 °C, and 4.8 ± 2.0 °C in the scenarios of no air circulation, natural air circulation, and combined natural and forced air circulation, respectively. The interaction of fan location and fan direction showed a significant effect on the compartment temperatures while there was no significant effect on cooling time. A circulation fan on the lower part of the 12-volt section with an air supply from the 12- to 110-volt section was determined as the optimal condition to achieve relatively uniform temperature distribution. Refrigeration system also achieved a cooling temperature of 7.2 °C within 150 min to meet regulations. To that end, the innovative hybrid oyster refrigeration system will benefit oyster industries, as well as the aquaculture farmers in terms of complying with regulations and energy savings. Full article

Poultry litter is one type of biomass and waste generated from the farming process. This study performed a performance and process analysis of poultry litter to energy using the lab-scale shell and tube heat exchanger (STHE) system along with a Stirling engine and a swirling fluidized bed combustor (SFBC). The effects of tube shape, flow direction, and water flow rates on water and trailer temperature changes were investigated during the poultry litter co-combustion process. Energy flow analysis and emissions were also studied. Results showed that the water outlet temperature of 62.8 $°\mathrm{C}$ in the twisted tube was higher than the straight tube case (58.3 $°\mathrm{C}$ ) after 130 min of the co-combustion process. It was found that the counter-current direction had higher water temperature changes, higher logarithmic mean temperature difference (LMTD), and higher trailer temperature changes than the co-current direction. A water flow rate of 4.54 L/min showed adequate heat absorption in the lab-scale STHE system and heat rejection in the trailer. Results indicated that the lab-scale STHE system has a conversion efficiency of 42.3% and produces hot water (at about 63.9 $°\mathrm{C}$ ) along with lower emissions. This research study confirmed that poultry litter can be used to generate energy (e.g., hot water and electricity) by using a lab-scale biomass conversion system for space heating applications. Full article

Red ginseng is one of the most popular traditional medicines in Korea because its soluble hot-water extract is known to be very effective on enhancing immunity as well as inhibiting inflammation. Recently, we developed a new technique, called the HACgearshift system, which can pulverize red ginseng into the ultra-fine granules ranging from 0.2 to 7.0 μm in size. In this study, the soluble hot-water extract of those ultra-fine granules of red ginseng (URG) was investigated and compared to that of the normal-sized granules of red ginseng (RG). The high pressure liquid chromatographic analyses of the soluble hot-water extracts of both URG and RG revealed that URG had about 2-fold higher amounts of the ginsenosides, the biologically active components in red ginseng, than RG did. Using quantitative RT-PCR, cytokine profiling against the Escherichia coli lipopolysaccharide (LPS) in the monocyte-derived macrophage THP-1 cells demonstrated that the URG-treated cells showed a significant reduction in cytokine expression than the RG-treated ones. Transcription expression of the LPS-induced cytokines such as TNF-α, IL-1β, IL-6, IL-8, IL-10, and TGF-β was significantly inhibited by URG compared to RG. These results suggest that some biologically active and soluble components in red ginseng can be more effectively extracted from URG than RG by standard hot-water extraction. Full article