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Authors = Marita Georgia Riccardi

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18 pages, 2714 KiB  
Article
A Preliminary Study on the Efficacy of Essential Oils Against Trichoderma longibrachiatum Isolated from an Archival Document in Italy
by Benedetta Paolino, Maria Cristina Sorrentino, Severina Pacifico, Maria Carmen Garrigos, Marita Georgia Riccardi, Rubina Paradiso, Ernesto Lahoz and Giorgia Borriello
Heritage 2025, 8(6), 187; https://doi.org/10.3390/heritage8060187 - 24 May 2025
Viewed by 603
Abstract
In this study, a historically significant journal subject to fungal colonization was used as a case study for experimenting with a fumigation treatment using essential oils. The experiments were carried out both in vitro and in vivo directly on the artifact. Post-treatment monitoring [...] Read more.
In this study, a historically significant journal subject to fungal colonization was used as a case study for experimenting with a fumigation treatment using essential oils. The experiments were carried out both in vitro and in vivo directly on the artifact. Post-treatment monitoring showed that the succession of two fumigation treatments (alternately using rosemary and lavender oil) resulted in the complete disinfection of the first and second populations detected on the substrate. The latter was identified as Trichoderma longibrachiatum, a human pathogenic species, which was found to be sensitive to various concentrations of rosemary essential oil (1.2% v/v) and lavender essential oil (0.4% v/v), while it was not contained by the standard biocide based on benzalkonium chloride. The results obtained allowed the proposal of an application protocol for the fumigation of paper items that need to undergo biocidal treatment, which consists of alternating essential oils to increase the action spectrum of the natural substances and implementing a rotation principle to prevent the development of bio-resistances. Full article
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7 pages, 715 KiB  
Communication
Whole-Genome Sequencing-Based Characterization of a Listeria monocytogenes Strain from an Aborted Water Buffalo in Southern Italy
by Rubina Paradiso, Marita Georgia Riccardi, Bianca Cecere, Nunzia Riccone, Roberto Scialla, Antonietta Anzalone, Anna Cerrone, Esterina De Carlo, Giorgia Borriello and Giovanna Fusco
Microorganisms 2021, 9(9), 1875; https://doi.org/10.3390/microorganisms9091875 - 3 Sep 2021
Cited by 4 | Viewed by 2654
Abstract
Listeria monocytogenes is a Gram-positive pathogen causing life-threatening infections both in humans and animals. In livestock farms, it can persist for a long time and primarily causes uterine infections and encephalitis in farmed animals. Whole genome sequencing (WGS) is currently becoming the best [...] Read more.
Listeria monocytogenes is a Gram-positive pathogen causing life-threatening infections both in humans and animals. In livestock farms, it can persist for a long time and primarily causes uterine infections and encephalitis in farmed animals. Whole genome sequencing (WGS) is currently becoming the best method for molecular typing of this pathogen due to its high discriminatory power and efficiency of characterization. This study describes the WGS-based characterization of an L. monocytogenes strain from an aborted water buffalo fetus in southern Italy. The strain under study was classified as molecular serogroup IVb, phylogenetic lineage I, MLST sequence type 6, Clonal Complex 6, and cgMLST type CT3331, sublineage 6. Molecular analysis indicated the presence of 61 virulence genes and 4 antibiotic resistance genes. Phylogenetic analysis, including all the publicly available European L. monocytogenes serogroup IVb isolates, indicated that our strain clusterized with all the other CC6 strains and that different CCs were variably distributed within countries and isolation sources. This study contributes to the current understanding of the genetic diversity of L. monocytogenes from animal sources and highlights how the WGS strategy can provide insights into the pathogenic potential of this microorganism, acting as an important tool for epidemiological studies. Full article
(This article belongs to the Special Issue Molecular Research of Zoonotic Infectious Diseases)
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12 pages, 2319 KiB  
Article
Fatal Interstitial Pneumonia Associated with Bovine Coronavirus in Cows from Southern Italy
by Maria Grazia Amoroso, Giuseppe Lucifora, Barbara Degli Uberti, Francesco Serra, Giovanna De Luca, Giorgia Borriello, Alessandro De Domenico, Sergio Brandi, Maria Concetta Cuomo, Francesca Bove, Marita Georgia Riccardi, Giorgio Galiero and Giovanna Fusco
Viruses 2020, 12(11), 1331; https://doi.org/10.3390/v12111331 - 19 Nov 2020
Cited by 13 | Viewed by 3812
Abstract
An outbreak of winter dysentery, complicated by severe respiratory syndrome, occurred in January 2020 in a high production dairy cow herd located in a hilly area of the Calabria region. Of the 52 animals belonging to the farm, 5 (9.6%) died with severe [...] Read more.
An outbreak of winter dysentery, complicated by severe respiratory syndrome, occurred in January 2020 in a high production dairy cow herd located in a hilly area of the Calabria region. Of the 52 animals belonging to the farm, 5 (9.6%) died with severe respiratory distress, death occurring 3–4 days after the appearance of the respiratory signs (caught and gasping breath). Microbiological analysis revealed absence of pathogenic bacteria whilst Real-time PCR identified the presence of RNA from Bovine Coronavirus (BCoV) in several organs: lungs, small intestine (jejunum), mediastinal lymph nodes, liver and placenta. BCoV was therefore hypothesized to play a role in the lethal pulmonary infection. Like the other CoVs, BCoV is able to cause different syndromes. Its role in calf diarrhea and in mild respiratory disease is well known: we report instead the involvement of this virus in a severe and fatal respiratory disorder, with symptoms and disease evolution resembling those of Severe Acute Respiratory Syndromes (SARS). Full article
(This article belongs to the Special Issue Enteric Viruses in Animals)
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13 pages, 2031 KiB  
Article
Analysis of XX SRY-Negative Sex Reversal Dogs
by Sara Albarella, Lisa De Lorenzi, Elena Rossi, Francesco Prisco, Marita Georgia Riccardi, Brunella Restucci, Francesca Ciotola and Pietro Parma
Animals 2020, 10(9), 1667; https://doi.org/10.3390/ani10091667 - 16 Sep 2020
Cited by 12 | Viewed by 132197
Abstract
Impaired fertility associated with disorders of sex development (DSDs) due to genetic causes in dogs are more and more frequently reported. Affected dogs are usually of specific breeds thus representing a cause of economic losses for breeders. The aim of this research is [...] Read more.
Impaired fertility associated with disorders of sex development (DSDs) due to genetic causes in dogs are more and more frequently reported. Affected dogs are usually of specific breeds thus representing a cause of economic losses for breeders. The aim of this research is to report the clinical, cytogenetic and molecular genetic findings of four XX SRY-negative DSD dog cases. All the subjects showed a female aspect and the presence of an enlarged clitoris with a penis bone. Morphopathological analyses performed in three of the four cases showed the presence of testes in two cases and ovotestis in another. Conventional and R-banded cytogenetic techniques were applied showing that no chromosome abnormalities were involved in these DSDs. CGH arrays show the presence of 11 copy number variations (CNVs), one of which is a duplication of 458 Kb comprising the genomic region between base 17,503,928 and base 17,962,221 of chromosome 9 (CanFam3 genome assembly). This CNV, confirmed also by qPCR, includes the promoter region of SOX9 gene and could explain the observed phenotype. Full article
(This article belongs to the Special Issue Congenital Malformation in Domestic Animals)
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14 pages, 1940 KiB  
Article
Different Impacts of MucR Binding to the babR and virB Promoters on Gene Expression in Brucella abortus 2308
by Giorgia Borriello, Veronica Russo, Rubina Paradiso, Marita Georgia Riccardi, Daniela Criscuolo, Gaetano Verde, Rosangela Marasco, Paolo Vincenzo Pedone, Giorgio Galiero and Ilaria Baglivo
Biomolecules 2020, 10(5), 788; https://doi.org/10.3390/biom10050788 - 19 May 2020
Cited by 13 | Viewed by 3186
Abstract
The protein MucR from Brucella abortus has been described as a transcriptional regulator of many virulence genes. It is a member of the Ros/MucR family comprising proteins that control the expression of genes important for the successful interaction of α-proteobacteria with their eukaryotic [...] Read more.
The protein MucR from Brucella abortus has been described as a transcriptional regulator of many virulence genes. It is a member of the Ros/MucR family comprising proteins that control the expression of genes important for the successful interaction of α-proteobacteria with their eukaryotic hosts. Despite clear evidence of the role of MucR in repressing virulence genes, no study has been carried out so far demonstrating the direct interaction of this protein with the promoter of its target gene babR encoding a LuxR-like regulator repressing virB genes. In this study, we show for the first time the ability of MucR to bind the promoter of babR in electrophoretic mobility shift assays demonstrating a direct role of MucR in repressing this gene. Furthermore, we demonstrate that MucR can bind the virB gene promoter. Analyses by RT-qPCR showed no significant differences in the expression level of virB genes in Brucella abortus CC092 lacking MucR compared to the wild-type Brucella abortus strain, indicating that MucR binding to the virB promoter has little impact on virB gene expression in B. abortus 2308. The MucR modality to bind the two promoters analyzed supports our previous hypothesis that this is a histone-like protein never found before in Brucella. Full article
(This article belongs to the Section Molecular Biology)
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