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Authors = Kwa Jia Sim

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10 pages, 2858 KiB  
Proceeding Paper
A Mini Literature Review on Current Advancements in Protein Purification Techniques
by Mohd Hairul Mohd Salleh, Aida Fitri Peli, Mohamad Syazwan Ngalimat and Kwa Jia Sim
Biol. Life Sci. Forum 2022, 20(1), 12; https://doi.org/10.3390/IECBM2022-13507 - 9 Nov 2022
Cited by 1 | Viewed by 6021
Abstract
Protein purification is an ever-vital technique for academia and industry. This paper mainly reviews and discusses one of the core components of proteomics—the latest advances in separation technology for protein components—focusing on five different methods. The multi-column plate adapter (MCPA) system is incredibly [...] Read more.
Protein purification is an ever-vital technique for academia and industry. This paper mainly reviews and discusses one of the core components of proteomics—the latest advances in separation technology for protein components—focusing on five different methods. The multi-column plate adapter (MCPA) system is incredibly economical for protein treatment research to purify samples. Because of the affinity of excess Ulp1 protease for SUMO fusion, excess protein products may be obtained within half an hour using this method. Magnetic separation strategies can offer a better protein purification process in the future because of a few advantages. The evaluation established that the aqueous two-phase system (ATPS) technique is a cost-effective, time-saving (30 min), and high-recuperation approach that can be scaled up for commercial purposes. Therefore, the ATPS may be a viable single-step separation purification method, moving away from multi-step purification such as the chromatography technique. Our review provides a technique capable of efficient protein purification. Full article
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19 pages, 1690 KiB  
Article
Extractive Fermentation for Recovery of Bacteriocin-Like Inhibitory Substances Derived from Lactococcus lactis Gh1 Using PEG2000/Dextran T500 Aqueous Two-Phase System
by Roslina Jawan, Sahar Abbasiliasi, Joo Shun Tan, Murni Halim, Shuhaimi Mustafa, Bin Hao Lee, Jia Sim Kwa and Arbakariya B. Ariff
Fermentation 2021, 7(4), 257; https://doi.org/10.3390/fermentation7040257 - 2 Nov 2021
Cited by 10 | Viewed by 3819
Abstract
This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). This system was developed for the simultaneous cell cultivation and downstream processing of [...] Read more.
This work aimed to optimize the parameters affecting partitioning of a bacteriocin-like inhibitory substances (BLIS) from Lactococcus lactis Gh1 in extractive fermentation using polyethylene glycol (PEG)/dextran aqueous two-phase system (ATPS). This system was developed for the simultaneous cell cultivation and downstream processing of BLIS. Results showed that the molecular weight of PEG, PEG concentration, and dextran T500 affect the partition coefficient (K), purification factor (PF), and yield of BLIS partitioning. ATPS composed of 10% (w/w) PEG2000 and 8% (w/w) dextran T500, provided the greatest conditions for the extractive BLIS production. The K (1.00 ± 0.16), PF (2.92 ± 0.37) and yield (77.24 ± 2.81%) were increased at selected orbital speed (200 rpm) and pH (pH 7). Sustainable growth of the cells in the bioreactor and repeated fermentation up to the eighth extractive batch were observed during the scale up process, ensuring a continuous production and purification of BLIS. Hence, the simplicity and effectiveness of ATPS in the purification of BLIS were proven in this study. Full article
(This article belongs to the Special Issue Fermentation and Bioactive Metabolites 3.0)
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