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Authors = Gražvydas Lukinavičius

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10 pages, 1682 KiB  
Article
Live Fluorescence Imaging of F-Actin Organization in Chick Whole Embryo Cultures Using SiR-Actin
by Manuel Schmitz-Elbers, Gražvydas Lukinavičius and Theodoor H. Smit
Cells 2021, 10(7), 1578; https://doi.org/10.3390/cells10071578 - 22 Jun 2021
Cited by 1 | Viewed by 4281
Abstract
Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation [...] Read more.
Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation and cytokinesis and mediate mechanical signaling. Here, we stain F-actin with the fluorogenic probe SiR-actin for live fluorescence imaging of whole chick embryos. We found that 50 nM SiR-actin in the culture medium is a safe and effective concentration for this purpose, as it provides high labeling density without inducing morphological malformations. Full article
(This article belongs to the Special Issue Organization and Function of Cellular Structural Networks)
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15 pages, 3284 KiB  
Article
Direct Visualization of Amlodipine Intervention into Living Cells by Means of Fluorescence Microscopy
by Christine Quentin, Rūta Gerasimaitė, Alexandra Freidzon, Levon S. Atabekyan, Gražvydas Lukinavičius, Vladimir N. Belov and Gyuzel Y. Mitronova
Molecules 2021, 26(10), 2997; https://doi.org/10.3390/molecules26102997 - 18 May 2021
Cited by 6 | Viewed by 4420
Abstract
Amlodipine, a unique long-lasting calcium channel antagonist and antihypertensive drug, has weak fluorescence in aqueous solutions. In the current paper, we show that direct visualization of amlodipine in live cells is possible due to the enhanced emission in cellular environment. We examined the [...] Read more.
Amlodipine, a unique long-lasting calcium channel antagonist and antihypertensive drug, has weak fluorescence in aqueous solutions. In the current paper, we show that direct visualization of amlodipine in live cells is possible due to the enhanced emission in cellular environment. We examined the impact of pH, polarity and viscosity of the environment as well as protein binding on the spectral properties of amlodipine in vitro, and used quantum chemical calculations for assessing the mechanism of fluorescence quenching in aqueous solutions. The confocal fluorescence microscopy shows that the drug readily penetrates the plasma membrane and accumulates in the intracellular vesicles. Visible emission and photostability of amlodipine allow confocal time-lapse imaging and the drug uptake monitoring. Full article
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2 pages, 158 KiB  
Editorial
Fluorescent Probes for Live Cell Imaging
by Zhijie Chen and Gražvydas Lukinavičius
Chemosensors 2018, 6(3), 41; https://doi.org/10.3390/chemosensors6030041 - 19 Sep 2018
Cited by 5 | Viewed by 4478
(This article belongs to the Special Issue Fluorescent Probes for Live Cell Imaging)
12 pages, 17730 KiB  
Review
The Use of Hoechst Dyes for DNA Staining and Beyond
by Jonas Bucevičius, Gražvydas Lukinavičius and Rūta Gerasimaitė
Chemosensors 2018, 6(2), 18; https://doi.org/10.3390/chemosensors6020018 - 18 Apr 2018
Cited by 159 | Viewed by 42730
Abstract
Hoechst dyes are among the most popular fluorophores used to stain DNA in living and fixed cells. Moreover, their high affinity and specificity towards DNA make Hoechst dyes excellent targeting moieties, which can be conjugated to various other molecules in order to tether [...] Read more.
Hoechst dyes are among the most popular fluorophores used to stain DNA in living and fixed cells. Moreover, their high affinity and specificity towards DNA make Hoechst dyes excellent targeting moieties, which can be conjugated to various other molecules in order to tether them to DNA. The recent developments in the fields of microscopy and flow cytometry have sparked interest in such composite molecules, whose applications range from investigating nucleus microenvironment to drug delivery into tumours. Here we provide an overview of the properties of Hoechst dyes and discuss recent developments in Hoechst-based composite probes. Full article
(This article belongs to the Special Issue Fluorescent Probes for Live Cell Imaging)
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