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Review

How to Choose the Right Inducible Gene Expression System for Mammalian Studies?

by
Tuula Kallunki
1,2,
Marin Barisic
3,4,
Marja Jäättelä
1,4 and
Bin Liu
1,*
1
Cell Death and Metabolism, Center for Autophagy, Recycling and Disease, Danish Cancer Society Research Center, 2100 Copenhagen, Denmark
2
Department of Drug Design and Pharmacology, Faculty of Health Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
3
Cell Division and Cytoskeleton, Danish Cancer Society Research Center, 2100 Copenhagen, Denmark
4
Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Cells 2019, 8(8), 796; https://doi.org/10.3390/cells8080796
Submission received: 28 June 2019 / Revised: 18 July 2019 / Accepted: 26 July 2019 / Published: 30 July 2019
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Abstract

Inducible gene expression systems are favored over stable expression systems in a wide variety of basic and applied research areas, including functional genomics, gene therapy, tissue engineering, biopharmaceutical protein production and drug discovery. This is because they are mostly reversible and thus more flexible to use. Furthermore, compared to constitutive expression, they generally exhibit a higher efficiency and have fewer side effects, such as cell death and delayed growth or development. Empowered by decades of development of inducible gene expression systems, researchers can now efficiently activate or suppress any gene, temporarily and quantitively at will, depending on experimental requirements and designs. Here, we review a number of most commonly used mammalian inducible expression systems and provide basic standards and criteria for the selection of the most suitable one.
Keywords: tetracycline; cumate; light-switchable; tamoxifen tetracycline; cumate; light-switchable; tamoxifen

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MDPI and ACS Style

Kallunki, T.; Barisic, M.; Jäättelä, M.; Liu, B. How to Choose the Right Inducible Gene Expression System for Mammalian Studies? Cells 2019, 8, 796. https://doi.org/10.3390/cells8080796

AMA Style

Kallunki T, Barisic M, Jäättelä M, Liu B. How to Choose the Right Inducible Gene Expression System for Mammalian Studies? Cells. 2019; 8(8):796. https://doi.org/10.3390/cells8080796

Chicago/Turabian Style

Kallunki, Tuula, Marin Barisic, Marja Jäättelä, and Bin Liu. 2019. "How to Choose the Right Inducible Gene Expression System for Mammalian Studies?" Cells 8, no. 8: 796. https://doi.org/10.3390/cells8080796

APA Style

Kallunki, T., Barisic, M., Jäättelä, M., & Liu, B. (2019). How to Choose the Right Inducible Gene Expression System for Mammalian Studies? Cells, 8(8), 796. https://doi.org/10.3390/cells8080796

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