Biophysical Methods to Study Membrane Models, Cells, and Tissues

A special issue of Biophysica (ISSN 2673-4125).

Deadline for manuscript submissions: 31 January 2026 | Viewed by 874

Special Issue Editor


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Guest Editor
1. Fondazione Policlinico Universitario A. Gemelli IRCCS, Largo A. Gemelli 8, 00168 Rome, Italy
2. Institute of Physics, Catholic University of Rome, Largo F. Vito 1, 00168 Rome, Italy
Interests: metabolic imaging; fluorescence microscopy; membrane biophysics; artificial intelligence; biophysical methods; instrumentation
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Special Issue Information

Dear Colleagues,

The study of membrane models, cells, and tissues provides critical insights into the structural and functional properties of biological membranes, which are central to numerous physiological and pathological processes. Advances in biophysical methods have significantly enhanced our ability to investigate the dynamic behavior and interactions of membranes at the molecular level. This Special Issue aims to highlight cutting-edge biophysical techniques and their applications in studying membrane models, ranging from lipid bilayers and vesicles to complex membrane systems. We invite original research articles, reviews, and methodological papers that address innovative approaches, novel findings, or comprehensive overviews in this field.

We hope that this special issue will serve as a platform to share advancements and foster collaboration among researchers working in membrane biophysics and related areas. Contributions from multidisciplinary perspectives, including physics, chemistry, biology, and computational modeling, are particularly encouraged.

Dr. Giuseppe Maulucci
Guest Editor

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Keywords

  • membrane models
  • biophysical methods
  • lipid bilayers
  • fluorescence microscopy
  • metabolic imaging
  • computational modeling

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Published Papers (1 paper)

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Research

26 pages, 2269 KB  
Article
Laser Trapping Technique for Measuring Ionization Energy and Identifying Hemoglobin Through Charge Quantification in Blood Samples
by Endris M. Endris, Deresse A. Adem, Horace T. Crogman and Daniel B. Erenso
Biophysica 2025, 5(4), 56; https://doi.org/10.3390/biophysica5040056 - 18 Nov 2025
Viewed by 367
Abstract
We present a proof-of-concept study using a laser trapping (LT) approach to characterize hemoglobin variants through controlled dielectric breakdown of red blood cell membranes. Using a 1064 nm infrared laser, we analyzed 62 cells from each of four hemoglobin types (Hb AS, Hb [...] Read more.
We present a proof-of-concept study using a laser trapping (LT) approach to characterize hemoglobin variants through controlled dielectric breakdown of red blood cell membranes. Using a 1064 nm infrared laser, we analyzed 62 cells from each of four hemoglobin types (Hb AS, Hb FA, Hb FSC, Hb AC), measuring the ionization time, cell area, and trap displacement to calculate the apparent threshold ionization energy (TIE*) and apparent threshold radiation dose (TRD*). Post-ionization trajectories and radiation intensity measurements provided charge distribution profiles for each variant. Our results indicate variant-specific differences in TRD* and charge-to-volume ratios across adults and infants (p < 0.05), while the TIE* values remained largely consistent. Charge analysis revealed statistically significant variation between some groups, suggesting that TRD* and charge-based parameters may offer sensitive markers of hemoglobin heterogeneity. This work demonstrates the feasibility of laser trapping as a complementary single-cell method for hemoglobin analysis. While limited in sample size, the approach highlights the potential of TIE* and TRD* measurements for differentiating hemoglobin variants and suggests future applications in hemoglobinopathy screening and diagnostic research. Full article
(This article belongs to the Special Issue Biophysical Methods to Study Membrane Models, Cells, and Tissues)
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