A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Pulp Exposure
2.3. In Vivo Micro-CT Imaging and Analysis
2.4. Histopathologic Analysis
2.5. Sampling, Genomic DNA Extraction, Amplification, and Sequencing
2.6. Taxonomic Assignment of Individual Sequencing Reads, Bioinformatics, and Statistical Analysis
3. Results
3.1. In Vivo Micro-CT Observations and Analysis
3.2. Histologic Features of Apical Periodontitis Progression
3.3. Richness of Bacterial Species
3.4. Comparison of Bacterial Communities
3.5. Pattern of Bacterial Community Based on Phylogenetic Analysis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Total Valid Reads | Average Read Lengths | No. of OTUs | Shannon Diversity Index | No. of Phylum | No. of Genus | No. of Species | |
---|---|---|---|---|---|---|---|
42-P1 | 65507 | 419.3 bp | 212 | 3.39 | 5 | 113 | 184 |
21-P1 | 72343 | 425.2 bp | 99 | 2.35 | 5 | 61 | 95 |
18-P1 | 70389 | 424.9 bp | 97 | 2.66 | 5 | 51 | 84 |
48-P2 | 65178 | 422.1 bp | 194 | 2.99 | 6 | 109 | 180 |
27-P2 | 71754 | 423.9 bp | 166 | 1.78 | 4 | 91 | 141 |
24-P2 | 66468 | 420.2 bp | 354 | 2.83 | 5 | 149 | 328 |
54-P3 | 64866 | 415.1 bp | 337 | 3.51 | 6 | 136 | 310 |
33-P3 | 67652 | 421.7 bp | 157 | 2.69 | 5 | 95 | 145 |
30-P3 | 89946 | 418.3 bp | 141 | 2.47 | 9 | 88 | 137 |
43-R1 | 74667 | 410.7 bp | 199 | 1.09 | 15 | 138 | 196 |
22-R1 | 76408 | 411.5 bp | 192 | 1.03 | 13 | 139 | 186 |
19-R1 | 67556 | 412.9 bp | 168 | 1.04 | 14 | 131 | 166 |
49-R2 | 64582 | 410.7 bp | 203 | 1.23 | 12 | 151 | 202 |
28-R2 | 84751 | 421.0 bp | 205 | 2.07 | 11 | 117 | 185 |
25-R2 | 79475 | 418.2 bp | 327 | 1.83 | 14 | 205 | 319 |
55-R3 | 76542 | 417.5 bp | 294 | 2.83 | 9 | 176 | 284 |
34-R3 | 68434 | 418.9 bp | 235 | 1.43 | 15 | 164 | 229 |
31-R3 | 80190 | 414.3 bp | 190 | 2.00 | 13 | 135 | 188 |
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Park, O.-J.; Jeong, M.-H.; Lee, E.-H.; Cho, M.-R.; Hwang, J.; Cho, S.; Yun, C.-H.; Han, S.H.; Kim, S.-Y. A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model. Microorganisms 2020, 8, 1174. https://doi.org/10.3390/microorganisms8081174
Park O-J, Jeong M-H, Lee E-H, Cho M-R, Hwang J, Cho S, Yun C-H, Han SH, Kim S-Y. A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model. Microorganisms. 2020; 8(8):1174. https://doi.org/10.3390/microorganisms8081174
Chicago/Turabian StylePark, Ok-Jin, Moon-Hee Jeong, Eun-Hye Lee, Mi-Ran Cho, Jaehong Hwang, Seungryong Cho, Cheol-Heui Yun, Seung Hyun Han, and Sun-Young Kim. 2020. "A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model" Microorganisms 8, no. 8: 1174. https://doi.org/10.3390/microorganisms8081174
APA StylePark, O.-J., Jeong, M.-H., Lee, E.-H., Cho, M.-R., Hwang, J., Cho, S., Yun, C.-H., Han, S. H., & Kim, S.-Y. (2020). A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model. Microorganisms, 8(8), 1174. https://doi.org/10.3390/microorganisms8081174