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Article

Validation of a Simple HPLC–UV Method for the Determination of Monomers Released from Dental Resin Composites in Artificial Saliva

by
Elisavet-Ioanna Diamantopoulou
1,
Orfeas-Evanggelos Plastiras
1,
Petros Mourouzis
2 and
Victoria Samanidou
1,*
1
Department of Chemistry, Laboratory of Analytical Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
2
Department of Dental Tissues Pathology and Therapeutics, Division of Operative Dentistry, Faculty of Dentistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
*
Author to whom correspondence should be addressed.
Methods Protoc. 2020, 3(2), 35; https://doi.org/10.3390/mps3020035
Submission received: 4 April 2020 / Revised: 28 April 2020 / Accepted: 29 April 2020 / Published: 3 May 2020
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Abstract

Bisphenol-A (BPA), bisphenol A glycerolate dimethacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), and urethane dimethacrylate (UDMA) are organic monomers that can be released from dental composites into the oral cavity. Over specific concentrations, they can act as endocrine disruptors or cause toxic effects. The purpose of this work is to develop and validate an analytical method to determine BPA, Bis-GMA, TEGDMA, and UDMA monomers released from synthetic dental resins in artificial saliva. The method was validated before its application to new hybrid ceramic materials used in computer-aided design and computer-aided manufacturing (CAD/CAM) restorations to determine the release of monomers in various time intervals (e.g., 24 h, and 7, 14, 30, and 60 days), both in methanolic solutions, as well as in artificial saliva. Chromatographic analysis was performed isocratically on a Perfect Sil Target ODS-3 analytical column (250 mm × 4.6 mm, 5 µm) with CH3CN/H2O, 58/42% v/v as mobile phase within 23 min. The developed method was validated in terms of selectivity, linearity, accuracy, and precision.
Keywords: validation; artificial saliva; bisphenol-A (BPA); bisphenol A glycerolate dimethacrylate (BIS-GMA); dental composites; CAD/CAM materials; dimethacrylate monomers; HPLC; triethylene glycol dimethacrylate (TEGDMA); urethane dimethacrylate (UDMA) validation; artificial saliva; bisphenol-A (BPA); bisphenol A glycerolate dimethacrylate (BIS-GMA); dental composites; CAD/CAM materials; dimethacrylate monomers; HPLC; triethylene glycol dimethacrylate (TEGDMA); urethane dimethacrylate (UDMA)

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MDPI and ACS Style

Diamantopoulou, E.-I.; Plastiras, O.-E.; Mourouzis, P.; Samanidou, V. Validation of a Simple HPLC–UV Method for the Determination of Monomers Released from Dental Resin Composites in Artificial Saliva. Methods Protoc. 2020, 3, 35. https://doi.org/10.3390/mps3020035

AMA Style

Diamantopoulou E-I, Plastiras O-E, Mourouzis P, Samanidou V. Validation of a Simple HPLC–UV Method for the Determination of Monomers Released from Dental Resin Composites in Artificial Saliva. Methods and Protocols. 2020; 3(2):35. https://doi.org/10.3390/mps3020035

Chicago/Turabian Style

Diamantopoulou, Elisavet-Ioanna, Orfeas-Evanggelos Plastiras, Petros Mourouzis, and Victoria Samanidou. 2020. "Validation of a Simple HPLC–UV Method for the Determination of Monomers Released from Dental Resin Composites in Artificial Saliva" Methods and Protocols 3, no. 2: 35. https://doi.org/10.3390/mps3020035

APA Style

Diamantopoulou, E.-I., Plastiras, O.-E., Mourouzis, P., & Samanidou, V. (2020). Validation of a Simple HPLC–UV Method for the Determination of Monomers Released from Dental Resin Composites in Artificial Saliva. Methods and Protocols, 3(2), 35. https://doi.org/10.3390/mps3020035

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