Impact of the Aryl Hydrocarbon Receptor on Aurora A Kinase and the G2/M Phase Pathway in Hematopoietic Stem and Progenitor Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice and In Vivo Treatment
2.2. Collection of Bone Marrow Cells
2.3. HSPC Isolation and Flow Cytometry
2.4. RNA Sequencing Library Construction and Transcriptomic Analysis
2.5. Statistical Analyses
2.6. Data Availability
3. Results
3.1. Absence of AHR Alters HSPC Transcriptome
3.2. Acute Loss or Antagonism of AHR Results in Increased AurA in Hematopoietic Stem and Progenitor Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Franchini, A.M.; Vaughan, K.L.; Bhattacharya, S.; Singh, K.P.; Gasiewicz, T.A.; Lawrence, B.P. Impact of the Aryl Hydrocarbon Receptor on Aurora A Kinase and the G2/M Phase Pathway in Hematopoietic Stem and Progenitor Cells. Receptors 2023, 2, 100-115. https://doi.org/10.3390/receptors2010006
Franchini AM, Vaughan KL, Bhattacharya S, Singh KP, Gasiewicz TA, Lawrence BP. Impact of the Aryl Hydrocarbon Receptor on Aurora A Kinase and the G2/M Phase Pathway in Hematopoietic Stem and Progenitor Cells. Receptors. 2023; 2(1):100-115. https://doi.org/10.3390/receptors2010006
Chicago/Turabian StyleFranchini, Anthony M., Keegan L. Vaughan, Soumyaroop Bhattacharya, Kameshwar P. Singh, Thomas A. Gasiewicz, and B. Paige Lawrence. 2023. "Impact of the Aryl Hydrocarbon Receptor on Aurora A Kinase and the G2/M Phase Pathway in Hematopoietic Stem and Progenitor Cells" Receptors 2, no. 1: 100-115. https://doi.org/10.3390/receptors2010006