Analysis of the Bacterial Community of Metal Scrap Using an Enrichment Culture Approach
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling and Enrichment Culture
2.2. Genomic DNA Extraction and PCR Conditions
2.3. Sequencing Library Preparation, Sequencing, and Bioinformatics Analysis
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Correction Statement
References
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OTU | Closest Relative | Accession No. | Identity (%) | Relative Abundance (%) | |
---|---|---|---|---|---|
Class | Species | ||||
Sphingomonas sp. 1 | α-Proteobacteria | Sphingomonas kyungheensis THG-B283T | NR_118263 | 99.1 | 7.1 |
Sphingomonas sp. 2 | α-Proteobacteria | Sphingomonas kyungheensis THG-B283T | NR_118263 | 99.1 | 5.6 |
Sphingomonas sp. 3 | α-Proteobacteria | Sphingomonas kyungheensis THG-B283T | NR_118263 | 99.1 | 4.8 |
Sphingomonas sp. 4 | α-Proteobacteria | Sphingomonas kyungheensis THG-B283T | NR_118263 | 99.3 | 4.6 |
Mucilaginibacter sp. 1 | Sphingobacteria | Mucilaginibacter terrae CCM 8645T | NR_158094 | 99.7 | 4.3 |
Sphingomonas sp. 5 | α-Proteobacteria | Sphingomonas kyungheensis THG-B283T | NR_118263 | 97.6 | 4.2 |
Sphingomonas sp. 6 | α-Proteobacteria | Sphingomonas kyungheensis THG-B283T | NR_118263 | 99.1 | 4.0 |
Methylobacterium sp. 1 | α-Proteobacteria | Methylobacterium mesophilicum JCM 2829T | NR_115550 | 99.1 | 3.7 |
Pseudomonas sp. 1 | γ-Proteobacteria | Pseudomonas koreensis Ps 9-14T | NR_025228 | 99.1 | 3.5 |
Sphingomonas sp. 7 | α-Proteobacteria | Sphingomonas kyungheensis THG-B283T | NR_118263 | 99.3 | 3.1 |
Sphingomonas sp. 8 | α-Proteobacteria | Sphingomonas kyungheensis THG-B283T | NR_118263 | 99.3 | 2.4 |
Sphingomonas sp. 9 | α-Proteobacteria | Sphingomonas kyungheensis THG-B283T | NR_118263 | 99.1 | 2.0 |
Massilia sp. 1 | β-Proteobacteria | Massilia agri K-3-1T | NR_157781 | 98.5 | 1.9 |
Methylobacterium sp. 2 | α-Proteobacteria | Methylobacterium bullatum F3.2T | NR_116548 | 98.2 | 1.4 |
Sphingomonas sp. 10 | α-Proteobacteria | Sphingomonas hankookensis ODN7T | NR_116570 | 98.6 | 1.3 |
Alkanindiges sp. 1 | γ-Proteobacteria | Alkanindiges hongkongensis HKU9T | NR_115178 | 97.4 | 1.2 |
Methylobacterium sp. 3 | α-Proteobacteria | Methylobacterium hispanicum DSM 16372T | NR_112613 | 99.1 | 1.1 |
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Akita, H.; Shinto, Y.; Kimura, Z.-i. Analysis of the Bacterial Community of Metal Scrap Using an Enrichment Culture Approach. Appl. Biosci. 2023, 2, 23-30. https://doi.org/10.3390/applbiosci2010004
Akita H, Shinto Y, Kimura Z-i. Analysis of the Bacterial Community of Metal Scrap Using an Enrichment Culture Approach. Applied Biosciences. 2023; 2(1):23-30. https://doi.org/10.3390/applbiosci2010004
Chicago/Turabian StyleAkita, Hironaga, Yoshiki Shinto, and Zen-ichiro Kimura. 2023. "Analysis of the Bacterial Community of Metal Scrap Using an Enrichment Culture Approach" Applied Biosciences 2, no. 1: 23-30. https://doi.org/10.3390/applbiosci2010004