Pathological Sequelae Associated with Skeletal Muscle Atrophy and Histopathology in G93A*SOD1 Mice
Abstract
:1. Introduction
2. Results
2.1. Skeletal Muscles of G93A*SOD1 Mice Showed Atrophy and a Differential Pattern of Mutant SOD1 Protein Expression
2.2. Reduced Endurance and Tolerance to Exercise in G93A*SOD1 Mice
2.3. Skeletal Muscles Collagen Deposition and Myopathy in G93A*SOD1 Mice
2.4. Skeletal Muscles Isolated from G93A*SOD1 Mice Showed Altered Skeletal Muscle Mitochondrial Transport and Antioxidant Enzyme Protein Levels
2.5. G93A*SOD1 Mice Skeletal Muscles Develop Mitochondrial Dysfunction
3. Discussion
4. Material and Methods
4.1. Materials
4.2. Animals
4.3. Muscle Endurance Capacity
4.4. Muscle Exercise Tolerance
4.5. Muscle Isolation and Morphometry
4.6. Protein Isolation and Western Blotting
4.7. Histological Analyses
4.8. WGA Staining
4.9. Immunohistochemistry
4.10. Transmission Electron Microscopy
4.11. Immunostaining
4.12. Mitochondrial Isolation and Respiration Assessment
4.13. Statistical Analysis and Reproducibility
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Aishwarya, R.; Abdullah, C.S.; Remex, N.S.; Nitu, S.; Hartman, B.; King, J.; Bhuiyan, M.A.N.; Rom, O.; Miriyala, S.; Panchatcharam, M.; et al. Pathological Sequelae Associated with Skeletal Muscle Atrophy and Histopathology in G93A*SOD1 Mice. Muscles 2023, 2, 51-74. https://doi.org/10.3390/muscles2010006
Aishwarya R, Abdullah CS, Remex NS, Nitu S, Hartman B, King J, Bhuiyan MAN, Rom O, Miriyala S, Panchatcharam M, et al. Pathological Sequelae Associated with Skeletal Muscle Atrophy and Histopathology in G93A*SOD1 Mice. Muscles. 2023; 2(1):51-74. https://doi.org/10.3390/muscles2010006
Chicago/Turabian StyleAishwarya, Richa, Chowdhury S. Abdullah, Naznin Sultana Remex, Sadia Nitu, Brandon Hartman, Judy King, Mohammad Alfrad Nobel Bhuiyan, Oren Rom, Sumitra Miriyala, Manikandan Panchatcharam, and et al. 2023. "Pathological Sequelae Associated with Skeletal Muscle Atrophy and Histopathology in G93A*SOD1 Mice" Muscles 2, no. 1: 51-74. https://doi.org/10.3390/muscles2010006
APA StyleAishwarya, R., Abdullah, C. S., Remex, N. S., Nitu, S., Hartman, B., King, J., Bhuiyan, M. A. N., Rom, O., Miriyala, S., Panchatcharam, M., Orr, A. W., Kevil, C. G., & Bhuiyan, M. S. (2023). Pathological Sequelae Associated with Skeletal Muscle Atrophy and Histopathology in G93A*SOD1 Mice. Muscles, 2(1), 51-74. https://doi.org/10.3390/muscles2010006