The Journey towards Solubility Assessment of Small Molecules Using HPLC-DAD †
by
Nada Hemmani
, 
Anaïs Cacilie Martin
,
Andy Larivière
,
Mathis Tresallet
,
Kensa Tchang
,
Isabelle Krimm
and
Marc Le Borgne
*
Small Molecules for Biological Targets Team, Centre de Recherche en Cancérologie de Lyon, Centre Léon Bérard, Institut National de la Santé et de la Recherche Médicale (INSERM) 1052, Centre National de la Recherche Scientifique (CNRS) 5286, Université Claude Bernard Lyon 1, 69373 Lyon, France
*
Author to whom correspondence should be addressed.
†
Presented at the 8th International Electronic Conference on Medicinal Chemistry, 1–30 November 2022; Available online: https://ecmc2022.sciforum.net/ .
Med. Sci. Forum 2022, 14(1), 62; https://doi.org/10.3390/ECMC2022-13143
Published: 1 November 2022
(This article belongs to the Proceedings of The 8th International Electronic Conference on Medicinal Chemistry)
Abstract
:In the process of developing bioactive small molecules, solubility determination is a crucial step. Many research papers treating problems related to solubility are published, but none of them fully describes the methods and steps for solubility assessment. In addition, in silico prediction tools and databases such as SwissADME, ACD/Percepta, DrugBank and many others offer the possibility to have approximative solubility values based on the structure of the molecule. Although significant differences can be observed depending on the database and the conditions of the experiment such as solvent, pH, temperature… etc. The lack of data can be a barrier to obtaining details on solubility measurement methods. This presentation aims to describe, step by step, the journey of tryptophan solubility determination using high-performance liquid chromatography-diode array detector (HPLC-DAD).
Supplementary Materials
The following are available online at https://www.mdpi.com/article/10.3390/ECMC2022-13143/s1.
Author Contributions
Conceptualization, I.K. and M.L.B.; methodology, N.H., A.C.M., A.L., M.T. and K.T.; investigation, M.T., A.L. and N.H.; supervision, M.L.B. All authors have read and agreed to the published version of the manuscript.
Funding
Marc Le Borgne and Isabelle Krimm would like to thank “Institut Convergence PLAsCAN” (ANR-17-CONV-0002).
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Not applicable.
Conflicts of Interest
The authors declare no conflict of interest.
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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
MDPI and ACS Style
Hemmani, N.; Martin, A.C.; Larivière, A.; Tresallet, M.; Tchang, K.; Krimm, I.; Borgne, M.L. The Journey towards Solubility Assessment of Small Molecules Using HPLC-DAD. Med. Sci. Forum 2022, 14, 62. https://doi.org/10.3390/ECMC2022-13143
AMA Style
Hemmani N, Martin AC, Larivière A, Tresallet M, Tchang K, Krimm I, Borgne ML. The Journey towards Solubility Assessment of Small Molecules Using HPLC-DAD. Medical Sciences Forum. 2022; 14(1):62. https://doi.org/10.3390/ECMC2022-13143
Chicago/Turabian StyleHemmani, Nada, Anaïs Cacilie Martin, Andy Larivière, Mathis Tresallet, Kensa Tchang, Isabelle Krimm, and Marc Le Borgne. 2022. "The Journey towards Solubility Assessment of Small Molecules Using HPLC-DAD" Medical Sciences Forum 14, no. 1: 62. https://doi.org/10.3390/ECMC2022-13143
