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Volume 14
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Abstract

Effect of Santolina chamaecyparissus on Physiological Parameters: Data from an Animal Model of Mammary Cancer †

by
Tiago Azevedo
1,*,
Jessica Silva
1,
Abigaël Valada
1,
Lara Anjos
1,
Ana Cristina Silvestre-Ferreira
2,3,
Tiane Cristine Finimundy
4,5,
Lillian Barros
4,5,
Manuela Matos
1,
Maria João Neuparth
6,7,
Maria João Pires
1 and
Paula A. Oliveira
1,8
1
Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
2
Animal and Veterinary Research Centre (CECAV), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
3
Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
4
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal
5
Laboratório Associado para a Sustentabilidade e Tecnologia em Regiões de Montanha (SusTEC), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal
6
Centro de Investigação em Atividade Física, Saúde e Lazer (CIAFEL), Faculdade de Desporto da Universidade do Porto (FADEUP), Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR), 4200-319 Porto, Portugal
7
TOXRUN—Unidade de Investigação em Toxicologia, Instituto Universitário de Ciências da Saúde, Cooperativa de Ensino Superior Politécnico e Universitário (CESPU), 4585-116 Gandra, Portugal
8
Academic Medical Center of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal
*
Author to whom correspondence should be addressed.
†
Presented at the 8th International Electronic Conference on Medicinal Chemistry, 1–30 November 2022; Available online: https://ecmc2022.sciforum.net/.
Med. Sci. Forum 2022, 14(1), 20; https://doi.org/10.3390/ECMC2022-13441
Published: 1 November 2022
(This article belongs to the Proceedings of The 8th International Electronic Conference on Medicinal Chemistry)
Versions Notes

Abstract

:
Breast cancer is the most common cancer worldwide. Santolina chamaecyparissus L. has successfully inhibited the MCF-7 cancer cell line. This study aims to evaluate the chemopreventive effects of S. chamaecyparissus aqueous extract (SCE) on female rats’ physiological parameters with mammary cancer induced by N-methyl-N-nitrosourea (MNU). The institutional ethics committee approved this study. Twenty-eight four-week-old female Wistar rats were divided into Control, MNU-induced (IND), SCE and SCE+IND. SCE was supplemented with drinking water (120 µg/mL). At 50 days of age, MNU was intraperitoneally administered. Humane endpoints were evaluated weekly. After twenty-one weeks, animals were sacrificed by ketamine/xylazine overdose and blood was collected. A complete blood count was performed using an automated haematology analyser. An autoanalyzer was used to measure serum markers (albumin, cholesterol, glucose and triglycerides). SCE’s chemical characterisation was performed by LC-MS, as it found nineteen phenolic compounds, the main molecules were myricetin-O-glucuronide and 1,3-O-dicaffeoylquinic acid. Regarding haemoglobin concentration, there was a difference (p = 0.050) between SCE and Control (16.38 ± 0.41 g/dL and 15.18 ± 0.29 g/dL, respectively). Mean Platelet Volume differed between SCE+IND (8.29 ± 0.15 fL) and IND (9.03 ± 0.26 fL) (p = 0.014). Platelet Distribution Width differed between 9.06 ± 0.14 fL (SCE + IND) and 10.58 ± 0.42 fL (IND) (p < 0.001), but also between SCE (8.78 ± 0.16 fL) and SCE + IND versus control (9.86 ± 0.17 fL) (p = 0.007 and p = 0.034, respectively). SCE had no effect on the humane endpoints or serum markers. Platelet size appears to have been significantly affected by SCE. SCE supplementation had no effect on liver or kidney function or the well-being of the animals, implying it could be a viable treatment option for breast cancer. Histological analysis will help confirm SCE’s toxicological profile.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/ECMC2022-13441/s1.

Author Contributions

Methodology and writing—original draft: T.A.; Investigation—T.A., J.S., A.V., L.A., T.C.F., A.C.S.-F., M.J.N., M.J.P.; conceptualization, supervision and project administration: L.B., M.M. and P.A.O. All authors have read and agreed to the published version of the manuscript.

Funding

The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through a Doctoral Grant (2020.07999.BD, J.S.), and through national funds FCT/MCTES (PIDDAC) to CITAB (UIDB/04033/2020), CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021); L.B. thanks to the national funding by FCT through the institutional scientific employment program–contract for her contract.

Institutional Review Board Statement

This study was approved by an Ethics Review Body (“ORBEA—Órgão Responsável pelo Bem-Estar e Ética Animal”) under reference 834-e-CITAB-2020.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data is contained within the article.

Conflicts of Interest

The authors declare no conflict of interest.
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Share and Cite

MDPI and ACS Style

Azevedo, T.; Silva, J.; Valada, A.; Anjos, L.; Silvestre-Ferreira, A.C.; Finimundy, T.C.; Barros, L.; Matos, M.; Neuparth, M.J.; Pires, M.J.; et al. Effect of Santolina chamaecyparissus on Physiological Parameters: Data from an Animal Model of Mammary Cancer. Med. Sci. Forum 2022, 14, 20. https://doi.org/10.3390/ECMC2022-13441

AMA Style

Azevedo T, Silva J, Valada A, Anjos L, Silvestre-Ferreira AC, Finimundy TC, Barros L, Matos M, Neuparth MJ, Pires MJ, et al. Effect of Santolina chamaecyparissus on Physiological Parameters: Data from an Animal Model of Mammary Cancer. Medical Sciences Forum. 2022; 14(1):20. https://doi.org/10.3390/ECMC2022-13441

Chicago/Turabian Style

Azevedo, Tiago, Jessica Silva, Abigaël Valada, Lara Anjos, Ana Cristina Silvestre-Ferreira, Tiane Cristine Finimundy, Lillian Barros, Manuela Matos, Maria João Neuparth, Maria João Pires, and et al. 2022. "Effect of Santolina chamaecyparissus on Physiological Parameters: Data from an Animal Model of Mammary Cancer" Medical Sciences Forum 14, no. 1: 20. https://doi.org/10.3390/ECMC2022-13441

APA Style

Azevedo, T., Silva, J., Valada, A., Anjos, L., Silvestre-Ferreira, A. C., Finimundy, T. C., Barros, L., Matos, M., Neuparth, M. J., Pires, M. J., & Oliveira, P. A. (2022). Effect of Santolina chamaecyparissus on Physiological Parameters: Data from an Animal Model of Mammary Cancer. Medical Sciences Forum, 14(1), 20. https://doi.org/10.3390/ECMC2022-13441

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