The Iridoids of In Vitro Propagated Nepeta cyrenaica Quézel & Zaffran †
by
, , , , ,
Ana Alimpić Aradski
1,*,
Danijela Mišić
2,
Uroš Gašić
2
,
Slađana Todorović
2,
Mariana Oalđe Pavlović
1,
Petar D. Marin
1,
Abdulhamed Giweli
3,4 and
Sonja Duletić-Laušević
1 1
Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia
2
Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia
3
Faculty of Science, University of Al-Zintan, Zintan 501, Libya
4
National Research Center for Tropical and Transboundary Diseases, Zintan 501, Libya
*
Author to whom correspondence should be addressed.
†
Presented at the 8th International Electronic Conference on Medicinal Chemistry, 1–30 November 2022; Available online: https://ecmc2022.sciforum.net/ .
Med. Sci. Forum 2022, 14(1), 147; https://doi.org/10.3390/ECMC2022-13148
Published: 1 November 2022
(This article belongs to the Proceedings of The 8th International Electronic Conference on Medicinal Chemistry)
Abstract
:Nepeta cyrenaica Quézel & Zaffran (Lamiaceae), an endemic species of the Libyan flora, has been scarcely explored until now. Since Nepeta species are proven as pharmacologically active plants rich in iridoid monoterpenes, this study was aimed to quantify the iridoids present in different extracts of in vitro propagated N. cyrenaica. The seeds collected from their natural habitat were germinated. One node stem explant of 5-week-old plants were used for plant micropropagation. After several cycles, the shoots were harvested. The extraction was performed using maceration procedure by dichloromehane, methanol, 96% ethanol, and hot distilled water. Subsequently, the extracts were subjected to UHPLC/(±)HESI-MS2 analysis of iridoids. Two iridoids were identified and quantified, namely epideoxyloganic acid and nepetalactol, as well as their precursor 8-oxogeranial. Iridoid glucoside epideoxyloganic acid was the most abundant in the methanolic and ethanolic extracts (1255.37 and 1262.78 µg/100 g dry extract), followed by aqueous extract. On the other hand, 8-oxogeranial and nepetalactol reached maximal amounts in the dichloromethane extract (476.80 and 1039.52 µg/100 g dry extract, respectively). The results of this study indicate that the high antioxidant and enzyme-inhibiting effects of dichloromethane extract confirmed in our previous study could be attributed to its iridoid content, which was particularly high due to the low polarity of this extraction solvent. In conclusion, endemic N. cyrenaica could be efficiently propagated in vitro as an iridoid-rich plant with great biological potential.
Supplementary Materials
The presentation material of this work is available online at https://www.mdpi.com/article/10.3390/ECMC2022-13148/s1.
Author Contributions
Conceptualization, A.A.A., M.O.P., S.D.-L.; methodology, D.M., S.T., A.A.A., M.O.P., A.G.; validation, D.M., S.D.-L.; formal analysis, A.A.A., D.M.; investigation, A.A.A., M.O.P., D.M., U.G., S.T.; resources, P.D.M.; data curation, A.A.A., M.O.P.; writing—original draft preparation, A.A.A.; writing—review and editing, A.A.A., P.D.M., M.O.P., D.M., U.G.; visualization, A.A.A.; supervision, S.D.-L.; project administration, S.D.-L., P.D.M.; funding acquisition, P.D.M. All authors have read and agreed to the published version of the manuscript.
Funding
This research was funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia [Grant No. 451-03-68/2022-14/200178 and 451-03-68/2022-14/200007].
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
The data presented in this study are available on request from the corresponding author. The data are not publicly available because they are part of the comprehensive study that is currently being prepared.
Conflicts of Interest
The authors declare no conflict of interest.
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Share and Cite
MDPI and ACS Style
Alimpić Aradski, A.; Mišić, D.; Gašić, U.; Todorović, S.; Oalđe Pavlović, M.; Marin, P.D.; Giweli, A.; Duletić-Laušević, S. The Iridoids of In Vitro Propagated Nepeta cyrenaica Quézel & Zaffran. Med. Sci. Forum 2022, 14, 147. https://doi.org/10.3390/ECMC2022-13148
AMA Style
Alimpić Aradski A, Mišić D, Gašić U, Todorović S, Oalđe Pavlović M, Marin PD, Giweli A, Duletić-Laušević S. The Iridoids of In Vitro Propagated Nepeta cyrenaica Quézel & Zaffran. Medical Sciences Forum. 2022; 14(1):147. https://doi.org/10.3390/ECMC2022-13148
Chicago/Turabian StyleAlimpić Aradski, Ana, Danijela Mišić, Uroš Gašić, Slađana Todorović, Mariana Oalđe Pavlović, Petar D. Marin, Abdulhamed Giweli, and Sonja Duletić-Laušević. 2022. "The Iridoids of In Vitro Propagated Nepeta cyrenaica Quézel & Zaffran" Medical Sciences Forum 14, no. 1: 147. https://doi.org/10.3390/ECMC2022-13148
