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The Antibiofilm Potential of Vapor Fractions of Selected Essential Oils against Pseudomonas aeruginosa  †

Department of Pharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-367 Wroclaw, Poland
Author to whom correspondence should be addressed.
Presented at the 2nd International Electronic Conference on Antibiotics—Drugs for Superbugs: Antibiotic Discovery, Modes of Action and Mechanisms of Resistance, 15–30 June 2022; Available online:
Med. Sci. Forum 2022, 12(1), 3;
Published: 15 June 2022


Biofilm is a major causative factor of persistent, hard-to-heal infections. Due to the biofilm’s persistence, even high-dose, systemic antibiotic therapy displays low antimicrobial efficacy. Therefore, there is an urgent need to search for novel bactericidal agents. Essential oils [EOs] are of great interest in this matter because of their broad spectrum of antimicrobial activity and non-specific mechanism of action. This study aimed to evaluate the antimicrobial activity of vapor fractions of three commercially available essential oils: menthol mint (Mentha arvensis, L.), lavender (Lavandula angustifolia, Mill.), and eucalyptus (Eucalyptus globulus, Labill.) against Pseudomonas aeruginosa biofilm. For the research purposes, fourteen clinical multidrug-resistant strains and one reference bacterial strain from American Type Culture Collection (ATCC 15441) were used. The self-developed evaluation method was applied, based on the optimized resazurin staining protocol, to determine the biofilm survival after the exposure to volatile agents. The results indicated that the EOs’ antibiofilm activity depends on the pseudomonal strains’ intraspecies variability. The lavender oil was active against five clinical strains, with the reduction rate ranging from 33% to 44%. The highest reduction level obtained for eucalyptus oil was 20%. The results for menthol mint oil were differentiated among the tested strains and ranged from 5% to 53%. The obtained outcomes indicate that the tested volatile fractions of EOs possess the potential to be applied in the treatment of infections caused by Pseudomonas aeruginosa biofilm. Further research is required to determine if they can be used in monotherapy or as adjuvants of other antimicrobial agents.

Supplementary Materials

The following are available online at

Author Contributions

Conceptualization, M.B. and A.J.; methodology, M.B. and J.P.; software, M.B.; validation, M.B. and A.J.; formal analysis, M.B. and A.J.; investigation, M.B. and J.P. and A.J.; resources, M.B. and A.J.; data curation M.B.; writing—original draft preparation, M.B.; writing—review and editing, M.B., J.P. and A.J.; visualization, M.B.; supervision, A.J.; project administration, M.B. and A.J.; funding acquisition, M.B. and A.J. All authors have read and agreed to the published version of the manuscript.


This research was funded by the National Science Centre, Poland (Grant No. 2021/41/N/NZ6/03305).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

All necessary data are presented in the manuscript and in the supplementary data and can be provided from the authors upon reasonable request.

Conflicts of Interest

The authors declare no conflict of interest.
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MDPI and ACS Style

Brożyna, M.; Paleczny, J.; Junka, A. The Antibiofilm Potential of Vapor Fractions of Selected Essential Oils against Pseudomonas aeruginosa . Med. Sci. Forum 2022, 12, 3.

AMA Style

Brożyna M, Paleczny J, Junka A. The Antibiofilm Potential of Vapor Fractions of Selected Essential Oils against Pseudomonas aeruginosa . Medical Sciences Forum. 2022; 12(1):3.

Chicago/Turabian Style

Brożyna, Malwina, Justyna Paleczny, and Adam Junka. 2022. "The Antibiofilm Potential of Vapor Fractions of Selected Essential Oils against Pseudomonas aeruginosa " Medical Sciences Forum 12, no. 1: 3.

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