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Volume 47, IECHo 2025
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Biol. Life Sci. Forum, 2025, ECM 2025

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9 pages, 734 KB  
Proceeding Paper
Comparative Evaluation of 16S rRNA and Housekeeping Gene-Specific Primer Pairs for Rhizobia and Agrobacteria Metagenomics
by Romain Kouakou Fossou and Adolphe Zézé
Biol. Life Sci. Forum 2025, 46(1), 1; https://doi.org/10.3390/blsf2025046001 - 2 Jul 2025
Viewed by 507
Abstract
Of many housekeeping genes, gyrB and rpoB are used as alternative markers to 16S rDNA to analyze Rhizobia and Agrobacteria communities. However, the extent to which the targeted genes and their corresponding primers could be suitable in metagenomic studies within communities belonging to [...] Read more.
Of many housekeeping genes, gyrB and rpoB are used as alternative markers to 16S rDNA to analyze Rhizobia and Agrobacteria communities. However, the extent to which the targeted genes and their corresponding primers could be suitable in metagenomic studies within communities belonging to the two taxa remains elusive. This work evaluates in silico the taxonomic resolution of partial regions of two housekeeping and 16S rRNA genes in differentiating between Rhizobia and Agrobacteria. The study confirmed V5–V7 as the best 16S rDNA variable region for differentiating all the genera at a 100% threshold. However, rpoB and gyrB markers outcompeted the 16S rDNA in terms of taxonomic resolution regardless of the threshold, possibly replacing the use of 16S rDNA V-regions in metagenomics studies of Rhizobia and Agrobacteria. Full article
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5 pages, 212 KB  
Proceeding Paper
Listeria monocytogenes Isolated from Fresh Pork Meat Commercialised in La Plata, Buenos Aires, Argentina
by Raúl E. Iza, Hernán D. Nievas, Camila Aurnague, Victorio F. Nievas, Santiago Urtizberea, Silvana M. Arce, Francisco Bucchianeri Faccin, Fabiana A. Moredo and Magdalena Costa
Biol. Life Sci. Forum 2025, 46(1), 2; https://doi.org/10.3390/blsf2025046002 - 9 Jul 2025
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Abstract
Listeria monocytogenes is one of the most significant foodborne pathogens capable of causing bacteremia. In the present study, a total of 138 pork meat samples were collected from 46 randomly selected butcher shops selling raw and ready-to-eat products in La Plata, Buenos Aires, [...] Read more.
Listeria monocytogenes is one of the most significant foodborne pathogens capable of causing bacteremia. In the present study, a total of 138 pork meat samples were collected from 46 randomly selected butcher shops selling raw and ready-to-eat products in La Plata, Buenos Aires, Argentina. Samples were processed according to internationally recognised standards, resulting in a positivity rate of 32.6%. This constitutes the first report of L. monocytogenes in fresh pork meat in the region and provides a strong foundation for control strategies of this microorganism in fresh pork meat retailers in Argentina. Full article
52 pages, 470 KB  
Conference Report
Abstracts of the 3rd International Electronic Conference on Microbiology
by Nico Jehmlich
Biol. Life Sci. Forum 2025, 46(1), 3; https://doi.org/10.3390/blsf2025046003 - 31 Jul 2025
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Abstract
The current proceedings summarize the presentations delivered during the third International Electronic Conference on Microbiology (ECM 2025), which was held online from 1 to 3 April 2025, via the SciForum platform. This virtual event brought together researchers from around the world to share [...] Read more.
The current proceedings summarize the presentations delivered during the third International Electronic Conference on Microbiology (ECM 2025), which was held online from 1 to 3 April 2025, via the SciForum platform. This virtual event brought together researchers from around the world to share recent advances in microbiological sciences. The ECM 2025 highlighted recent developments across a broad spectrum of microbiological research, including antimicrobial resistance, gut microbiota, infectious diseases, and environmental microbiomes. Participants shared their work through online presentations and abstracts, with selected submissions invited for full publication. The event fostered global collaboration, promoted open-access science, and showcased innovative tools for studying and managing microbial systems in health, agriculture, and industry. The multidisciplinary program was organized into several thematic sessions: S1. Gut Microbiota and Health Disease. S2. Foodborne Pathogens and Food Safety. S3. Antimicrobial Agents and Resistance. S4. Emerging Infectious Diseases. S5. Microbiome and Soil Science. S6. Microbial Characterization and Bioprocess. S7. Microbe–Plant Interactions. This conference report presents summaries of the contributions made by participating authors over the three-day event. Full article
5 pages, 361 KB  
Proceeding Paper
Detection and Species Distribution of Fungal Pathogens Associated with Otomycosis in a Tertiary Health Center in Kano, Nigeria
by Khadija Muhammad Kawu, Muhammad Ibrahim Getso, Taysir Ramadan, Alhassan Abdullahi Sharif, Kanishka Hrishi Das, Al-Mukhtar Yahuza Adamu and Mansur Aliyu
Biol. Life Sci. Forum 2025, 46(1), 4; https://doi.org/10.3390/blsf2025046004 - 22 Aug 2025
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Abstract
Otomycosis is the commonest cause of otitis externa seen in otorhinolaryngology clinics, especially in tropical and subtropical regions of the world. Diagnosis is usually made clinically and confirmed by laboratory identification of fungal elements. We conducted a cross-sectional and hospital-based epidemiological study that [...] Read more.
Otomycosis is the commonest cause of otitis externa seen in otorhinolaryngology clinics, especially in tropical and subtropical regions of the world. Diagnosis is usually made clinically and confirmed by laboratory identification of fungal elements. We conducted a cross-sectional and hospital-based epidemiological study that aimed to determine the prevalence, distribution and antifungal susceptibility profile of common aetiologic agents of otomycosis in Kano, northwestern Nigeria. We collected clinical samples from 300 patients who presented with symptoms of external ear infection, out of which 117 subjects (39.0%) were mycologically positive. Aspergillus species were the predominant isolate, with 90 (77.0%), followed by Candida species at 27 (23.0%). Otomycosis accounts for 39% of otitis externa seen in our center, mostly presenting with pruritus and otalgia. Raising the awareness of the general population to the predisposing factors may significantly reduce the incidence of otomycosis. Full article
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