Development of Highly Sensitive Anti-Mouse HER2 Monoclonal Antibodies for Flow Cytometry
, Tomohiro Tanaka 2, Mika K. Kaneko 2 and Yukinari Kato 1,2,*Round 1
Reviewer 1 Report
ijtm-2526141, Development of Highly Sensitive Anti-Mouse Epidermal Growth Factor Receptor 2 Monoclonal Antibodies for flow cytometry
The manuscript presents an interesting communication that fits the journal’s scope. The research seems to be conducted correctly and the results are interpreted accordingly. The manuscript is written with the proper scientific rigor, but is also easy to follow.
There are some minor editing problems. For example, the use of 10-9M and nM concentrations (figure 4A). It would be simpler for the reader to use only one style.
ijtm-2526141, Development of Highly Sensitive Anti-Mouse Epidermal Growth Factor Receptor 2 Monoclonal Antibodies for flow cytometry
The manuscript presents an interesting communication that fits the journal’s scope. The research seems to be conducted correctly and the results are interpreted accordingly. The manuscript is written with the proper scientific rigor, but is also easy to follow.
There are some minor editing problems. For example, the use of 10-9M and nM concentrations (figure 4A). It would be simpler for the reader to use only one style.
English is good.
Author Response
ijtm-2526141, Development of Highly Sensitive Anti-Mouse Epidermal Growth Factor Receptor 2 Monoclonal Antibodies for flow cytometry
The manuscript presents an interesting communication that fits the journal’s scope. The research seems to be conducted correctly and the results are interpreted accordingly. The manuscript is written with the proper scientific rigor, but is also easy to follow.
There are some minor editing problems. For example, the use of 10-9M and nM concentrations (figure 4A). It would be simpler for the reader to use only one style.
According to the comment, we revised Figure 4.
We used only 10-9M
Reviewer 2 Report
A communication "Development of Highly Sensitive Anti-Mouse Epidermal Growth Factor Receptor 2 Monoclonal Antibodies for flow cytometry" by Tsunenori Ouchida et al. describes the development of mHER2 mAbs using the Cell-Based Immunization and Screening (CBIS) method. The manuscript is written well and clearly. The results are presented and described concisely. The figures are prepared very clearly. The Authors sufficiently discussed the results based on available literature, which has been chosen appropriately. The conclusions are supported by the data. Overal, the manuscript presents novel discovery, which may be used in future studies.
Author Response
Thank you very much for the comment.
