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Review

A Comparison of Methods for the Production of Kilobase-Length Single-Stranded DNA

by
Chang-Yong Oh
1,* and
Eric R. Henderson
2
1
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA
2
Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA
*
Author to whom correspondence should be addressed.
DNA 2022, 2(1), 56-67; https://doi.org/10.3390/dna2010005
Submission received: 3 January 2022 / Revised: 14 February 2022 / Accepted: 18 February 2022 / Published: 1 March 2022
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Abstract

DNA nanoengineering, in particular, DNA origami has potential applications in a variety of areas including, for example, nanoelectronics, biomedical diagnostics, and therapeutics. To fully realize the potential of DNA self-assembly in these and other areas, methods must be available for economical, scalable, and reliable production of single-stranded DNA (ssDNA) scaffolds from virtually any source. In this review, we will describe the virtues and liabilities of four strategies for generating ssDNA, including Rolling Circle Amplification (RCA), strand-specific exonuclease digestion, chemical denaturation, and asymmetric PCR (aPCR), with suggestions for approaches to optimize the use of each method.
Keywords: DNA nanoengineering; DNA origami; scaffold; rolling circle amplification; lambda exonuclease; chemical denaturation; asymmetric polymerase chain reaction DNA nanoengineering; DNA origami; scaffold; rolling circle amplification; lambda exonuclease; chemical denaturation; asymmetric polymerase chain reaction
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MDPI and ACS Style

Oh, C.-Y.; Henderson, E.R. A Comparison of Methods for the Production of Kilobase-Length Single-Stranded DNA. DNA 2022, 2, 56-67. https://doi.org/10.3390/dna2010005

AMA Style

Oh C-Y, Henderson ER. A Comparison of Methods for the Production of Kilobase-Length Single-Stranded DNA. DNA. 2022; 2(1):56-67. https://doi.org/10.3390/dna2010005

Chicago/Turabian Style

Oh, Chang-Yong, and Eric R. Henderson. 2022. "A Comparison of Methods for the Production of Kilobase-Length Single-Stranded DNA" DNA 2, no. 1: 56-67. https://doi.org/10.3390/dna2010005

APA Style

Oh, C.-Y., & Henderson, E. R. (2022). A Comparison of Methods for the Production of Kilobase-Length Single-Stranded DNA. DNA, 2(1), 56-67. https://doi.org/10.3390/dna2010005

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