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Review

Quality Control of Cell Lines Using DNA as Target

Andalusian Public Health System Biobank, Coordinating Node, 18016 Granada, Spain
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Michael Fried
DNA 2022, 2(1), 44-55; https://doi.org/10.3390/dna2010004
Received: 19 December 2021 / Revised: 5 February 2022 / Accepted: 9 February 2022 / Published: 16 February 2022
Cell lines are a widely used pre-clinical models for biomedical research. The accessibility and the relative simplicity of facilities necessary for the use of cell lines, along with the large number of potential applications, encourage many researchers to choose this model. However, the access to cell lines from a non-confident source or through the interlaboratory exchange results in uncontrollable cell lines of uncertain quality. Furthermore, the possibility of using cell lines as an endless resource through multiple passages can contribute to this uncontrolled scenario, the main consequence of which is the lack of reproducibility between the research results. Different initiatives have emerged to promote the best practices regarding the use of cell lines and minimize the effect on the scientific results reported, including comprehensive quality control in the frame of Good Cell Culture Practice (GCCP). Cell Banks, research infrastructures for the professional distribution of biological material of high and known quality and origin, are committed with these initiatives. Many of the quality controls used to test different attributes of cell lines are based on DNA. This review describes quality control protocols of cell lines whose target molecule is DNA, and details the scope or purpose and their corresponding functionality. View Full-Text
Keywords: cell lines; good cell culture practice; DNA-based quality control; Cell Banks cell lines; good cell culture practice; DNA-based quality control; Cell Banks
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MDPI and ACS Style

Carrillo-Ávila, J.A.; Catalina, P.; Aguilar-Quesada, R. Quality Control of Cell Lines Using DNA as Target. DNA 2022, 2, 44-55. https://doi.org/10.3390/dna2010004

AMA Style

Carrillo-Ávila JA, Catalina P, Aguilar-Quesada R. Quality Control of Cell Lines Using DNA as Target. DNA. 2022; 2(1):44-55. https://doi.org/10.3390/dna2010004

Chicago/Turabian Style

Carrillo-Ávila, José A., Purificación Catalina, and Rocío Aguilar-Quesada. 2022. "Quality Control of Cell Lines Using DNA as Target" DNA 2, no. 1: 44-55. https://doi.org/10.3390/dna2010004

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