4.1. TRL Estimation of Industry 4.0 Applications in Expanding Feedstock Availability and Diversity
Following a comprehensive investigation encompassing breeding, land use mapping, and the integration of biomass into crop systems associated with biomass feedstock, the results are presented in
Table 4. The findings for each are discussed in the following. In the category focused on breeding, applications such as advanced genomic selection tools and high-throughput phenotyping, enabled by ML algorithms, sensor-based imaging, and big data analytics, enhance the ability to identify and develop biomass crops with improved yields, stress tolerance, and adaptability. Genomic selection refers to a breeding approach for plants or animals, in which the behaviors and performance of the offspring are predicted based on their DNA [
54]. In this approach, rather than awaiting outcomes, scientists utilize statistical methods to predict the anticipated characteristics of offspring before they are born. Regarding high-throughput phenotyping, it is a technique employed for assessing plant characteristics through the use of advanced technologies, including sensors, drones, and cameras [
55]. Life-cycle assessments (LCAs) can also benefit from the integration of data from IoT, digital twins, and AI [
56]. LCA is a systematic process that evaluates the environmental impacts of a product, process, or service throughout its entire life cycle, from raw material extraction to production, use, and disposal or recycling [
56]. When constantly supplied with data from the previously mentioned technology combination, it is known as dynamic LCA. This combination can be useful in collecting data from the plant and simulating the cultivation of emerging biomass feedstock under various scenarios, considering different uncertainties, which results in a robust measurement of their generated carbon footprint. These technology-enabled approaches can strengthen the sustainability, resilience, and productivity of biomass supply chains by ensuring a reliable flow of high-quality feedstock.
In the land use mapping category, applications like precision geospatial analysis and remote sensing, supported by drones, satellite imagery, and geographic information systems (GIS), enable the accurate identification of suitable cultivation areas, monitoring land use changes, and assessment of environmental impacts. These capabilities, driven by Industry 4.0 tools, support informed decision-making for biomass deployment while safeguarding environmental sustainability.
In the third category of actions needed within this action area, which involves integrating biomass into crop systems, applications such as intercropping models and rotational planning, powered by simulation software, IoT-enabled field sensors, and data integration platforms can help design cropping systems that incorporate biomass without compromising food production [
57,
58]. This integration, underpinned by digital technologies, enables optimization of land productivity, diversification of farm income, and improved soil health, contributing to the long-term sustainability of biomass supply chains.
Table 4.
Summary of TRL estimation of Industry 4.0 technology applications in breeding (a), land use mapping (b), and integration of biomass into crop systems (c) (see
Appendix Table A1 for detailed operational functions related to each case of application).
Table 4.
Summary of TRL estimation of Industry 4.0 technology applications in breeding (a), land use mapping (b), and integration of biomass into crop systems (c) (see
Appendix Table A1 for detailed operational functions related to each case of application).
| Industry 4.0 Technology | Application | TRL | References |
---|
(a) | Artificial Intelligence (AI) | Genomic Selection | 4–5 | [59,60] |
IoT, Robotics & Remote Sensing (UAVs) | High-throughput phenotyping | 6 | [61,62] |
Digital Twins & IoT | Dynamic LCA | 4–5 | [63,64] |
(b) | AI & Satellite Remote Sensing | Yield-Mapping and Land Optimization | 6 | [65] |
Remote Sensing (Satellite Imagery & GIS) & ML | Biomass and Land use Mapping | 4–5 | [37,66] |
(c) | Autonomous Robotics & AI | Inter-seeding Biomass Cover Crops | 7–8 | [57] |
AI & Big Data Analytics | Biomass Cover Crop Decision Support | 4–5 | [58] |
The TRL results for this action area reveal distinct patterns in technological maturity across the three focus areas, including breeding, land use mapping, and crop system integration. In breeding, AI-driven genomic selection is in the technology development phase, reflecting that algorithm development is progressing faster than multi-location validation and trait portability. A pilot in Brazil applied ML models to predict sugarcane biomass traits from genomic data, achieving 20–30% faster breeding cycles in controlled field trials, though scalability remains limited by dataset heterogeneity [
59].
Conversely, high-throughput phenotyping using UAV/IoT sensing has progressed to the technology demonstration stage, indicating that integrated prototypes are being used under realistic conditions. For Digital Twins and IoT in dynamic LCA (TRL 4–5), a conceptual model for European wood biomass supply chains integrates IoT sensors with virtual simulations to evaluate carbon intensity [
64]. This approach shows a potential 15% reduction in life-cycle emissions in lab-validated scenarios, although further field integration is needed.
For land use mapping, AI combined with satellite yield-mapping platforms is at the technology demonstration level. However, more generic biomass-type classifiers remain in the technology development phase owing to domain shift and labeling constraints that impact their ability to generalize. In remote sensing and ML for biomass land use mapping (TRL 4–5), a case study in northern China used Sentinel-2 and Landsat-9 imagery with random forest algorithms to map energy crop potential, identifying 10–20% more viable land than traditional methods, albeit with validation confined to small regional datasets [
66].
In crop-system integration, autonomous inter-seeding of biomass cover crops is already in the system commissioning stage. This high level of maturity is supported by repeated operational trials and early commercialization efforts. Conversely, for AI and big data in biomass cover crop decision support (TRL 4–5), a web-based tool (SEABEM) leveraged ML on African field data to predict cover crop biomass yields, improving farmer decisions in pilot farms by 25%, though reliant on limited training data [
58].
These patterns demonstrate that hardware-centric, narrowly scoped tools with well-defined feedback mechanisms are maturing at a faster pace than data-centric, system-level analytics that rely on interoperable datasets. In the near term, stakeholders should focus on deploying sensing and automation technologies to mitigate operational risks, while also instrumenting workflows to generate high-quality data. In the medium term, investments should be directed toward establishing common data standards, enabling cross-site benchmarking, and integrating analytics more closely with operational and economic constraints to advance lower-TRL models toward pilot readiness.
4.2. Readiness Levels of Industry 4.0 Technologies in Biomass Logistics Systems
Table 5 summarizes the TRL assessment for Industry 4.0 applications in three key logistics improvements: (a) designing regional depots, (b) advancing densification, and (c) optimizing transportation networks. The findings for each are discussed in the following. In the category of designing regional depots, applications such as pellet-delivery systems and pelletization are fundamental to improving the efficiency of biomass preprocessing and distribution, by extending sourcing distance beyond traditional limits and enabling the use of diverse or low-yield biomass types [
67,
68]. Industry 4.0 technologies, including discrete-event simulation, digital twins, and IoT-based monitoring, can optimize depot layout, coordinate material flows, and assess operational performance under varying demand and supply conditions [
69]. These advancements enhance regional supply reliability and reduce logistical bottlenecks, supporting the scalability of biomass supply chains [
70,
71].
In the category of advancing densification, applications like pellet dimension monitoring are essential for ensuring consistent product quality, which directly affects handling, storage, and combustion performance [
72]. Technologies such as computer vision, RFID-enabled quality tracking, and cloud-based analytics allow real-time measurement and control of pellet characteristics, enabling early detection of deviations and maintaining uniformity across large production batches [
72,
73]. This precision improves downstream processing efficiency and strengthens overall supply chain resilience.
In the category of optimizing transportation networks, applications like route optimization are essential for reducing delivery costs, minimizing emissions, and improving timeliness [
74,
75]. Industry 4.0 tools, including GIS-based routing software, AI-driven logistics planning, sensor-equipped transport vehicles, and digital twins, support dynamic decision-making by considering real-time traffic, weather, and supply conditions [
76,
77,
78]. These innovations enhance supply chain flexibility and sustainability, ensuring that biomass reaches end users efficiently [
75].
Table 5.
Summary of TRL estimation of Industry 4.0 technology applications in designing regional depots (a), advancing densification (b), and optimizing transportation networks (c) (see
Appendix Table A2 for detailed operational functions related to each case of application).
Table 5.
Summary of TRL estimation of Industry 4.0 technology applications in designing regional depots (a), advancing densification (b), and optimizing transportation networks (c) (see
Appendix Table A2 for detailed operational functions related to each case of application).
| Industry 4.0 Technology | Application | TRL | References |
---|
(a) | Digital Twin & Simulation | Advanced Pellet-delivery System | 4–5 | [13,75,79] |
(b) | Computer Vision & Automation | Smart Process Control and Quality Monitoring | 6 | [80] |
(c) | IoT, RFID & Cloud Analytics | IoT-based Tracking and Coordination | 4–5 | [19,81] |
AI & GIS | Route Optimization | 4–5 | [82] |
IoT & Digital Twins | Real-time LCA of Logistics Operations | 4–5 | [78,83] |
The maturity levels in logistics applications differ based on the extent to which each tool is integrated with existing operations. The development of digital twin technology and discrete-event simulation for regional depots is still at an early stage, where a U.S.-based model simulated wood pellet supply chains, optimizing depot layouts and reducing costs by 10–15% in virtual scenarios validated against historical data, though full-scale deployment awaits primarily due to models lacking validated data on variable feedstock flows, seasonal shocks, and depot behaviors during disruptions [
75]. Progress in densification, including pellet size monitoring, is currently in the demonstration phase. Vision systems are seamlessly retrofitted onto existing lines, providing immediate QA/QC results and requiring limited cross-site data sharing. IoT, RFID, and cloud tracking technologies are within the development level (TRL 4–5), where a prototype chipless RFID system for biomass logs in Australia enabled real-time provenance tracking in lab tests, improving traceability accuracy by 20%, but was limited by scalability in rural environments due to connectivity issues, device durability concerns, and data-sharing policies [
19]. Concurrently, AI-enabled GIS route optimization remains at the development stage due to fragmented fleets, limited real-time signals related to weights, moisture content [
84], and delays, as well as siloed dispatch systems, which diminish model effectiveness and external validity. Moreover, Industry 4.0 technologies, such as IoT and digital twins for real-time LCA (TRL 4–5) were piloted in Norwegian wood supply chains, simulating emissions reductions of 12% via symbiotic networks, though confined to conceptual validations [
78].
Near-term improvements can be achieved by deploying inline vision systems for pellet QA/QC and implementing phased IoT/RFID tracking on high-volume routes, supported by dashboards and APIs for real-time load monitoring. Pilot depots streaming process and flow data into digital twins can validate cost and reliability impacts through controlled tests. Routing optimization should incorporate operational constraints, with dynamic rerouting adopted as sensor coverage expands. Prioritizing interoperability through shared data models, event logs, and minimal vendor lock-in will enable lower-TRL analytics to progress by utilizing data from higher-TRL sensing and automation hardware (from development to demonstration). This approach can also help connect logistics performance with sustainability outcomes.
4.3. TRL Evaluation of Industry 4.0 Tools for Enhancing Feedstock Quality and Stability
Table 6 summarizes the TRL assessment for Industry 4.0 applications in three key logistics improvements: (a) enhancing drying and stabilization methods, (b) setting quality benchmarks, and (c) devising storage solutions. The findings for each are discussed in the following. In improving drying and stabilization methods, applications such as smart drying and near-infrared (NIR) spectroscopy are vital for enhancing the efficiency and consistency of biomass moisture reduction. Smart drying systems, supported by IoT sensors, AI-driven control algorithms, and real-time monitoring, can adjust drying parameters dynamically to reduce energy consumption while maintaining quality [
68,
85]. NIR spectroscopy provides a rapid, non-destructive assessment of biomass composition and moisture content, enabling precise control over stabilization processes and ensuring readiness for long-term storage or conversion [
86].
In setting quality benchmarks, applications like thermal monitoring and moisture monitoring play a crucial role in maintaining high product standards across the biomass supply chain [
87]. Thermal monitoring, often enabled by infrared imaging and embedded sensors, helps detect overheating or microbial activity that may degrade biomass quality [
88]. Moisture monitoring systems, integrated with cloud analytics platforms, allow continuous data collection and trend analysis to ensure compliance with quality specifications. In addition to conventional indicators of feedstock quality, there is a growing need for sustainability-oriented benchmarks that capture environmental performance [
64]. Emerging Industry 4.0 technologies, such as IoT sensor networks and digital twins, enable the generation of real-time data on resource use, emissions, and process conditions across biomass supply chains. When linked with LCA, these technologies enable the creation of dynamic benchmarks that extend beyond physical fuel properties to include carbon intensity and other environmental metrics [
63]. Together, these technologies provide an evidence-based approach to standard setting and verification [
87].
In developing storage solutions, similar monitoring technologies applied in the context of storage facilities support the prevention of biomass degradation during extended holding periods. Smart storage environments equipped with environmental sensors, predictive analytics, and automated ventilation systems can maintain optimal temperature and humidity conditions, thereby reducing spoilage and loss [
89,
90,
91]. These innovations help preserve the economic value of biomass and ensure supply consistency across seasons.
Table 6.
Summary of TRL estimation of Industry 4.0 technology applications in improving drying/stabilization methods (a), setting quality benchmarks (b), and developing storage solutions (c) (see
Appendix Table A3 for detailed operational functions related to each case of application).
Table 6.
Summary of TRL estimation of Industry 4.0 technology applications in improving drying/stabilization methods (a), setting quality benchmarks (b), and developing storage solutions (c) (see
Appendix Table A3 for detailed operational functions related to each case of application).
| Industry 4.0 Technology | Application | TRL | References |
---|
(a) | IoT sensors & AI control | Smart drying | 6 | [92,93] |
(b) | (Near-infrared) NIR Spectroscopy & ML | Predict Key Quality Parameters of Biomass | 7–8 | [94,95] |
IoT & Digital Twins | Dynamic LCA for Environmental Benchmarking | 4–5 | [64] |
(c) | IoT Thermal Monitoring | Thermal Monitoring | 9 | [96] |
Sensor Networks & Tomography | Moisture Monitoring | 4–5 | [84] |
Maturity in this action area is clearly tiered. Closed-loop smart drying is in the demonstration stage, already delivering repeatable gains in energy use. Predicting key quality parameters of biomass using NIR spectroscopy is at the system commissioning stage, validated by repeated use in QA/QC. For IoT and digital twins in dynamic LCA (TRL 4–5), a European wood biomass pilot utilized sensor data to simulate environmental benchmarks, resulting in a 10–15% reduction in simulated carbon intensity in controlled tests [
64]. Storage-focused thermal monitoring is at the final stage, with proven deployments for early heat-spot detection and fire-risk mitigation. By contrast, for sensor networks and tomography in moisture monitoring (TRL 4–5), a U.K. study deployed capacitive and acoustic sensors in wood piles, mapping internal moisture distributions with 85% accuracy in lab prototypes, but still costly and unproven at scale [
84]. Together, the results show that sensing/automation tied to immediate operational feedback advances faster than measurement concepts that require new hardware footprints and complex inference.
Practically, near-term value comes from standardizing intake and inline QA/QC with NIR, instrumenting dryers with closed-loop control (publishing key performance indicators (KPIs) such as kWh per percentage of moisture removed and rework rate), and deploying risk-based thermal surveillance in storage with clear alarm thresholds and response standard operating procedures (SOPs) [
86,
97]. Regarding the integration of digital twins with LCA, a broader adoption will depend on establishing standardized data protocols, interoperability, and cross-sector collaboration. In parallel, it appears necessary to focus research and development on moisture tomography at high-loss sites by conducting controlled pilot studies, comparing detection times with traditional probes, and measuring loss prevention to justify scale-up. All sensor outputs, such as IDs, timestamps, and moisture/temperature traces, should be routed into one shared data layer so that benchmarked datasets can support the development of lower-TRL concepts toward pilot readiness and facilitate cross-site learning.
4.4. Technology Readiness of Real-Time Monitoring Systems for Feedstock Quality
Table 7 summarizes the TRL assessment for Industry 4.0 applications in three key logistics improvements: (a) deployment of sensors and portable analyzers, and (b) setting quality establishment of real-time data integration platforms. The findings for each are discussed in the following. An analysis of biomass feedstock activities, including the deployment of sensors and portable analyzers and the establishment of real-time data integration platforms, is summarized in
Table 7. In improving the preprocessing efficiency of biomass, applications such as automated material handling and sensor-based contamination detection help streamline biomass preparation steps before conversion [
98]. Automated material handling, supported by robotics, conveyor automation, and IoT-enabled tracking systems, reduces manual labor, minimizes handling losses, and improves throughput [
99]. Sensor-based contamination detection, integrated with AI-driven image recognition and spectroscopy systems, ensures only clean, quality feedstock proceeds to further processing, reducing equipment wear and improving overall system efficiency [
100].
In enhancing feedstock uniformity, applications like particle size monitoring and blending optimization address the challenge of variability in biomass properties [
101]. Particle size monitoring systems, often equipped with machine vision or laser-based measurement tools, help maintain consistency critical for downstream processing [
102]. Blending optimization, guided by predictive analytics and process simulation models, enables precise mixing of different biomass sources to achieve desired quality parameters and improve conversion yields [
103].
In streamlining logistics integration, applications such as supply chain synchronization and digital twin modeling improve coordination between preprocessing facilities and downstream processing plants [
104]. Supply chain synchronization tools, powered by cloud-based platforms, facilitate real-time communication between stakeholders, while digital twin modeling creates virtual replicas of preprocessing operations to simulate and optimize logistical decisions [
105]. These approaches reduce bottlenecks, improve inventory control, and ensure the timely delivery of preprocessed biomass to conversion facilities.
Table 7.
Summary of TRL estimation of Industry 4.0 technology applications in deploying sensors and portable analyzers (a) and establishing real-time data integration platforms (b) (see
Appendix Table A4 for detailed operational functions related to each case of application).
Table 7.
Summary of TRL estimation of Industry 4.0 technology applications in deploying sensors and portable analyzers (a) and establishing real-time data integration platforms (b) (see
Appendix Table A4 for detailed operational functions related to each case of application).
| Industry 4.0 Technology | Application | TRL | References |
---|
(a) | Portable NIR Spectroscopy | On-site moisture content prediction | 9 | [106,107] |
Machine Vision (Digital Cameras & AI) | Real-time classification of biomass quality | 6 | [102] |
(b) | IoT Sensors & Wireless Telemetry | Real-time moisture monitoring and logistical decision-making | 7–8 | [108] |
Cloud-Based Analytics & IoT | Automatic sampling | 9 | [109] |
Results in
Table 7 disclose a maturity disparity between point-of-use sensing and the digital infrastructure. Portable analyzers and automated sampling have reached system operation, delivering decision-ready measurements supported by established SOPs and routine audits. Inline machine vision is appropriate during the technology demonstration phase, where accuracy and reliability are highly sensitive to factors such as dust, lighting, and sample presentation. However, the implementation of controlled enclosures and standardized illumination is advancing pilot projects [
110,
111]. Conversely, the digital infrastructure remains underdeveloped: real-time IoT telemetry for moisture and condition-aware decision-making is currently in the commissioning phase, whereas integration and traceability platforms are still in development. Pilot programs continue to demonstrate benefits for traceability and exception management [
108], but challenges such as interoperability, inconsistent connectivity, diverse device protocols, and data governance issues have impeded the transition from prototypes to routine operational deployment [
112,
113].
In the near term, the priority should be placed on point-of-use testing at intakes and critical control points (CCPs) [
114]. It is also important to standardize the calibration procedures and instrument high-loss nodes with simple alerts and operator standard operating procedures. A minimal viable data layer, comprising shared IDs, timestamps, and lot/batch links, can be established with offline-first buffering, enabling sites with weak connectivity to still capture signals [
115]. In the medium term, to enhance platform maturity from technology demonstration to commissioning, it is essential to secure integrations to a few high-value gates (intake release, re-route, re-dry) and run controlled A/B pilots, where two parallel pilot projects under similar conditions with one key variable altered are compared regarding their performance [
116]. Furthermore, curating labeled datasets enables analytics to transition from descriptive to predictive.
4.5. TRL Assessment of Industry 4.0-Enabled Planning and Forecasting Tools
Table 8 provides a summary of the TRL assessment concerning Industry 4.0 applications in two critical enhancements: (a) spatiotemporal forecasting, and (b) the integration of models with TEAs. The individual findings pertaining to each are elaborated subsequently. In the development of spatiotemporal forecasting tools, applications such as forecasting under uncertainty and accurate sensor-enabled yield forecasting strengthen decision-making processes within biomass supply chains [
18]. Forecasting under uncertainty, facilitated by stochastic modeling and ML algorithms, enables stakeholders to anticipate variations in feedstock availability driven by weather conditions, market dynamics, or supply disruptions [
117,
118]. Accurate sensor-enabled yield forecasting, utilizing remote sensing technologies, UAV-based imaging, and IoT-connected field sensors, enhances the accuracy of biomass yield predictions, thereby supporting improved harvest scheduling and supply planning [
119,
120]. These forecasting capabilities contribute to risk mitigation, optimize resource allocation, and improve supply chain resilience [
79].
Regarding the linkage of models to TEAs, applications such as integrating stochastic models with TEAs serve to bridge the gap between technical performance data and economic feasibility assessments. By synthesizing simulation outputs with cost–benefit models, stakeholders can evaluate the economic viability of various biomass production and processing scenarios across different contexts [
114,
115]. This linkage, supported by big data analytics and cloud-based modeling platforms, aids in identifying the most cost-effective strategies for scaling biomass supply chains, whilst accounting for uncertainties inherent in production, processing, and market demand.
Table 8.
Summary of TRL estimates of Industry 4.0 technology applications in creating spatiotemporal forecasting tools (a) and linking models to TEAs (b) (see
Appendix Table A5 for detailed operational functions related to each case of application).
Table 8.
Summary of TRL estimates of Industry 4.0 technology applications in creating spatiotemporal forecasting tools (a) and linking models to TEAs (b) (see
Appendix Table A5 for detailed operational functions related to each case of application).
| Industry 4.0 Technology | Application | TRL | References |
---|
(a) | Stochastic Modeling | Forecasting under uncertainty | 2–3 | [121] |
IoT Sensors & ML | Accurate sensor-enabled yield forecasting | 7–8 | [122] |
(b) | Simulations & Stochastic TEA | Linking stochastic models to TEA | 4–5 | [82,123] |
The maturity of spatiotemporal forecasting tools exhibits variability when evaluated along the six-stage continuum. Sensor-enabled yield forecasting has advanced to the system commissioning phase, with qualified deployments in operational environments. Nevertheless, their applicability across different crops, soils, and seasons remains limited by domain shift and small training datasets. Conversely, stochastic and scenario-based forecasting under uncertainty remain in the research stage. Prototype systems provide probabilistic outputs and calibrated intervals; however, end-to-end pipelines for ingest, systematic backtesting to validate predictive accuracy against historical data [
124], and reliability diagnostics are still in the research stage, rendering forecasts advisory rather than operational. Linkages to TEAs are situated within technological development. A U.S. gasification case study incorporated supply uncertainties, yielding probabilistic cost estimates with 15–25% variance in minimum selling price (MSP), validated in small-scale economic models [
82].While Monte Carlo simulations and scenario linkages are available, they are predominantly conceptual or case-specific and lack standardized integration into operational decision-making processes.
Recent priorities include enhancing data infrastructure, such as implementing consistent identifiers, timestamps, and feature stores, as well as institutionalizing backtesting protocols that utilize accuracy metrics, including bias, MAE/MAPE, reliability diagrams, and lead-time reporting. To facilitate decision-making under uncertainty, forecasts should consistently deliver probabilistic outputs with calibrated intervals rather than mere point estimates. Medium-term initiatives involve conducting controlled A/B pilot studies linked to operational KPIs such as supply shortfalls, re-drying rates, inventory levels, and cost per ton delivered. Connecting forecasts to TEA necessitates the propagation of forecast distributions into economic models and the derivation of decision-ready indicators such as net present value (NPV) ranges, breakeven prices, cost-of-shortfall, and emissions per ton [
123,
125]. Integrating these processes at pivotal stages—namely, intake release, rerouting, and re-drying—will ensure that uncertainty is systematically incorporated into both operational and investment planning. Ultimately, to advance forecasting and TEA linkages towards technology demonstration and commissioning, robust data practices are vital, including maintaining versioned datasets to ensure reproducibility, documenting data lineage for transparency, testing scalability via multi-site deployments, and preserving human oversight throughout the process [
126].