L-Asparaginase-Based Biosensors
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CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
2
Laboratory of Separation and Reaction Engineering-Laboratory of Catalysis and Materials (LSRE-LCM), Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal
3
Department of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Universidade Estadual Paulista-UNESP, Araraquara 14800-903, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Tak-Wah Wong
Encyclopedia 2021, 1(3), 848-858; https://doi.org/10.3390/encyclopedia1030065
Received: 24 June 2021
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Revised: 31 July 2021
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Accepted: 13 August 2021
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Published: 20 August 2021
(This article belongs to the Section Material Sciences)
L-asparaginase (ASNase) is an aminohydrolase enzyme widely used in the pharmaceutical and food industries. Although currently its main applications are focused on the treatment of lymphoproliferative disorders such as acute lymphoblastic leukemia (ALL) and acrylamide reduction in starch-rich foods cooked at temperatures above 100 °C, its use as a biosensor in the detection and monitoring of L-asparagine levels is of high relevance. ASNase-based biosensors are a promising and innovative technology, mostly based on colorimetric detection since the mechanism of action of ASNase is the catalysis of the L-asparagine hydrolysis, which releases L-aspartic acid and ammonium ions, promoting a medium pH value change followed by color variation. ASNase biosensing systems prove their potential for L-asparagine monitoring in ALL patients, along with L-asparagine concentration analysis in foods, due to their simplicity and fast response.
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Keywords:
L-asparaginase; biosensor; L-asparagine; monitoring; ammonia; pharmaceutical; food; industry
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MDPI and ACS Style
Nunes, J.C.F.; Cristóvão, R.O.; Santos-Ebinuma, V.C.; Faria, J.L.; Silva, C.G.; Neves, M.C.; Freire, M.G.; Tavares, A.P.M. L-Asparaginase-Based Biosensors. Encyclopedia 2021, 1, 848-858. https://doi.org/10.3390/encyclopedia1030065
AMA Style
Nunes JCF, Cristóvão RO, Santos-Ebinuma VC, Faria JL, Silva CG, Neves MC, Freire MG, Tavares APM. L-Asparaginase-Based Biosensors. Encyclopedia. 2021; 1(3):848-858. https://doi.org/10.3390/encyclopedia1030065
Chicago/Turabian StyleNunes, João C. F., Raquel O. Cristóvão, Valéria C. Santos-Ebinuma, Joaquim L. Faria, Cláudia G. Silva, Márcia C. Neves, Mara G. Freire, and Ana P. M. Tavares. 2021. "L-Asparaginase-Based Biosensors" Encyclopedia 1, no. 3: 848-858. https://doi.org/10.3390/encyclopedia1030065
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