Population Dynamics in the Biogenesis of Single-/Multi-Layered Membrane Vesicles Revealed by Encapsulated GFP-Monitoring Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Culture Conditions
2.2. Quantification of the Intracellular PHB Accumulation
2.3. Quantification of Extracellular MV Production and GFP Encupsulation into MVs
2.4. Cross-Sectional TEM Analysis
3. Results and Discussion
3.1. Working Hypothesis of PIA-MVP as a Regulatory Secretion Machinery of m-MVs
3.2. Direct Evidence of Encapsulation of GFP Reporter Protein into MVs
3.3. Is the Selective/regulatory Secretion of s-MV Possible?
3.4. Significance and Implication
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Koh, S.; Noda, S.; Taguchi, S. Population Dynamics in the Biogenesis of Single-/Multi-Layered Membrane Vesicles Revealed by Encapsulated GFP-Monitoring Analysis. Appl. Microbiol. 2023, 3, 1027-1036. https://doi.org/10.3390/applmicrobiol3030070
Koh S, Noda S, Taguchi S. Population Dynamics in the Biogenesis of Single-/Multi-Layered Membrane Vesicles Revealed by Encapsulated GFP-Monitoring Analysis. Applied Microbiology. 2023; 3(3):1027-1036. https://doi.org/10.3390/applmicrobiol3030070
Chicago/Turabian StyleKoh, Sangho, Shuhei Noda, and Seiichi Taguchi. 2023. "Population Dynamics in the Biogenesis of Single-/Multi-Layered Membrane Vesicles Revealed by Encapsulated GFP-Monitoring Analysis" Applied Microbiology 3, no. 3: 1027-1036. https://doi.org/10.3390/applmicrobiol3030070