Next Article in Journal
Brought to Light: How Ultraviolet Disinfection Can Prevent the Nosocomial Transmission of COVID-19 and Other Infectious Diseases
Previous Article in Journal
Prevalence of invA Gene of Salmonella spp. in Fish and Fishery Resources from Manila Bay Aquaculture Farms Using Real-Time PCR
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Applied Microbiology
Volume 1
Issue 3
10.3390/applmicrobiol1030034
applmicrobiol-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Bioassay-Guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-Resistant Staphylococcus aureus

by
Suwaiba Ladan Muhammad
1,*,
Yusuf Wada
2,3,
Mustapha Mohammed
4,5,*,
Sani Ibrahim
6,
Kabir Yusuf Musa
7,
Olayeni Stephen Olonitola
8,
Mubarak Hussaini Ahmad
9,
Sagir Mustapha
9,10,
Zaidah Abdul Rahman
2,11 and
Abubakar Sha’aban
4,5
1
Department of Chemical Science, Federal University of Kashere, Gombe PMB 0182, Gombe State, Nigeria
2
Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia
3
Department of Zoology, Faculty of Life Sciences, Ahmadu Bello University, Zaria 810107, Kaduna, Nigeria
4
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Pulau Pinang, Malaysia
5
Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria 810107, Kaduna, Nigeria
6
Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria 810107, Kaduna, Nigeria
7
Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria 810107, Kaduna, Nigeria
8
Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria 810107, Kaduna, Nigeria
9
Department of Pharmacology and Therapeutics, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria 810107, Kaduna, Nigeria
10
Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia
11
Hospital Universiti Sains Malaysia, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia
 
add Show full affiliation list
 
remove Hide full affiliation list
*
Authors to whom correspondence should be addressed.
Appl. Microbiol. 2021, 1(3), 520-536; https://doi.org/10.3390/applmicrobiol1030034
Submission received: 9 September 2021 / Revised: 25 October 2021 / Accepted: 2 November 2021 / Published: 15 November 2021
Browse Figures
Versions Notes

Abstract

Senna alata (Linn) Roxb. plant is widely used to manage various infections in folkloric medicine. Methicillin-resistant Staphylococcus aureus (MRSA) infection continues to be a major global public health problem. This study aims to investigate the bioactive components of S. alata leaves active against MRSA. The leaves of S. alata were sequentially extracted and fractionated using standard methods and screened for activities against MRSA. The diethyl ether active thin layer chromatography (TLC) spot was subjected to infrared (IR) and gas chromatography-mass spectroscopic (GC-MS) studies. The aqueous extract and diethyl ether fraction of S. alata leaves elicited the highest activity against the MRSA. The GC-MS analysis of the fraction produced 15 eluates; only the sub-fraction 13 was effective. The TLC analysis of the sub-fraction 13 revealed three spots; only the second spot produced activity. The GC-MS result of the spot showed six peaks. The spectral results for peak 3 match the data from the IR study, suggestive of 9-octadecenoic acid methyl ester. Senna alata leaves possess bioactive compounds closely related to 9-octadecenoic acid methyl ester with potent antibacterial activity against MRSA.
Keywords: bioassay; chromatography; folkloric medicine; methicillin-resistant Staphylococcus aureus; Senna alata; spectroscopy bioassay; chromatography; folkloric medicine; methicillin-resistant Staphylococcus aureus; Senna alata; spectroscopy

Share and Cite

MDPI and ACS Style

Muhammad, S.L.; Wada, Y.; Mohammed, M.; Ibrahim, S.; Musa, K.Y.; Olonitola, O.S.; Ahmad, M.H.; Mustapha, S.; Abdul Rahman, Z.; Sha’aban, A. Bioassay-Guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-Resistant Staphylococcus aureus. Appl. Microbiol. 2021, 1, 520-536. https://doi.org/10.3390/applmicrobiol1030034

AMA Style

Muhammad SL, Wada Y, Mohammed M, Ibrahim S, Musa KY, Olonitola OS, Ahmad MH, Mustapha S, Abdul Rahman Z, Sha’aban A. Bioassay-Guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-Resistant Staphylococcus aureus. Applied Microbiology. 2021; 1(3):520-536. https://doi.org/10.3390/applmicrobiol1030034

Chicago/Turabian Style

Muhammad, Suwaiba Ladan, Yusuf Wada, Mustapha Mohammed, Sani Ibrahim, Kabir Yusuf Musa, Olayeni Stephen Olonitola, Mubarak Hussaini Ahmad, Sagir Mustapha, Zaidah Abdul Rahman, and Abubakar Sha’aban. 2021. "Bioassay-Guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-Resistant Staphylococcus aureus" Applied Microbiology 1, no. 3: 520-536. https://doi.org/10.3390/applmicrobiol1030034

APA Style

Muhammad, S. L., Wada, Y., Mohammed, M., Ibrahim, S., Musa, K. Y., Olonitola, O. S., Ahmad, M. H., Mustapha, S., Abdul Rahman, Z., & Sha’aban, A. (2021). Bioassay-Guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-Resistant Staphylococcus aureus. Applied Microbiology, 1(3), 520-536. https://doi.org/10.3390/applmicrobiol1030034

Article Metrics

Back to TopTop