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Article

Enumeration of Brettanomyces in Wine Using Impedance

1
Department of Chemical and Materials and Engineering, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
2
LEAF, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Fani Mantzouridou
Appl. Microbiol. 2021, 1(2), 352-360; https://doi.org/10.3390/applmicrobiol1020024
Received: 3 July 2021 / Revised: 18 August 2021 / Accepted: 20 August 2021 / Published: 23 August 2021
Brettanomyces bruxellensis is a wine spoilage concern in wineries around the world. In order to maintain wine quality during storage and ageing, it is imperative to control and monitor this yeast. Being a fastidious slow growing yeast, which requires 5 to 14 days of incubation for visible growth in agar plates, it is difficult to detect growth (colonies) by conventional agar plate count method. Yeast enumeration by impedance was investigated because previous research using other microorganisms has shown that it is potentially faster than plate counting. The relationship between plate counting and impedance detection times was investigated for Brettanomyces inoculated in red wine samples. A linear relationship between log plate count concentrations and impedance detection times was found. Incubation time was reduced from 120 h down to 0.9 and 57.7 h for samples with 6.7 × 107 and 1.8 × 102 cfu/mL, respectively, using the ‘indirect’ impedance method. The ‘direct’ method also reduced the incubation times to 9.5 and 81.9 h, for the same concentrations. The ‘indirect’ impedance method has the potential to be used by the wine industry to control and monitor the Brettanomyces numbers in wines. View Full-Text
Keywords: Brettanomyces bruxellensis; Dekkera bruxellensis; spoilage; microbial detection; analytical method; quality control Brettanomyces bruxellensis; Dekkera bruxellensis; spoilage; microbial detection; analytical method; quality control
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MDPI and ACS Style

van Wyk, S.; Silva, F.V.M. Enumeration of Brettanomyces in Wine Using Impedance. Appl. Microbiol. 2021, 1, 352-360. https://doi.org/10.3390/applmicrobiol1020024

AMA Style

van Wyk S, Silva FVM. Enumeration of Brettanomyces in Wine Using Impedance. Applied Microbiology. 2021; 1(2):352-360. https://doi.org/10.3390/applmicrobiol1020024

Chicago/Turabian Style

van Wyk, Sanelle, and Filipa V. M. Silva. 2021. "Enumeration of Brettanomyces in Wine Using Impedance" Applied Microbiology 1, no. 2: 352-360. https://doi.org/10.3390/applmicrobiol1020024

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