Marker Haplotype Construction for the Hybrid Necrosis Gene Ne2 and Its Distribution in Old and New Wheat Varieties
Abstract
1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Simple PCR Marker Analysis for Lr13 and Ne2
2.3. Marker Haplotype Construction for Ne2 Using SNP Markers from Genotyping Arrays
3. Results
3.1. Phenotypic Screens for Hybrid Necrosis
3.2. Marker Analyses
3.3. Ne2 Haplotype Construction
3.4. Ne2 Prediction in Traditional and Modern Wheat Varieties and Change in Ne2 Distribution over Time
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
SNP | Single nucleotide polymorphism |
PCR | Polymerase chain reaction |
KASP | Kompetitive Allele Specific PCR |
CAPS | Cleaved amplified polymorphic sequence |
WW | Winter wheat |
SW | Spring wheat |
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Postulated Genes a | No. of Lines | HBAU-Lr13 b | Lr13haplo c | Observed Ne2 Haplotypes d |
---|---|---|---|---|
Ne2 | 22 | R | A | AAA |
15 | S | B | AAA | |
1 e | S | Z | ACA/G | |
ne2 | 14 | S | Z | AAA/AAG f, AA/CG, ACA |
9 | S | - | AAG, ACA, AC*, *C*, GAG | |
1 g | S | B | NA | |
Lr13 data only | 27 | R | A | AAA |
1 h | S | - | GA* | |
lr13 data only | 2 | S | A | AAA |
1 | S | Z | NA | |
4 | S | - | AC*, GAG | |
Lr other data only | 2 | R | A | AAA |
1 | S | Z | NA | |
2 | S | - | AC* | |
Ne2/ne2 carrier lines | 3 | S | - | GAG |
No data available | 10 | R | A | AAA |
3 | S | B | AAA | |
6 | S | Z | AAG, ACA, AC*, GA* | |
7 | S | - | ACA, GAG, GA* |
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Mohler, V.; Bund, A.; Hartl, L.; Albrecht, T. Marker Haplotype Construction for the Hybrid Necrosis Gene Ne2 and Its Distribution in Old and New Wheat Varieties. Crops 2025, 5, 36. https://doi.org/10.3390/crops5030036
Mohler V, Bund A, Hartl L, Albrecht T. Marker Haplotype Construction for the Hybrid Necrosis Gene Ne2 and Its Distribution in Old and New Wheat Varieties. Crops. 2025; 5(3):36. https://doi.org/10.3390/crops5030036
Chicago/Turabian StyleMohler, Volker, Adalbert Bund, Lorenz Hartl, and Theresa Albrecht. 2025. "Marker Haplotype Construction for the Hybrid Necrosis Gene Ne2 and Its Distribution in Old and New Wheat Varieties" Crops 5, no. 3: 36. https://doi.org/10.3390/crops5030036
APA StyleMohler, V., Bund, A., Hartl, L., & Albrecht, T. (2025). Marker Haplotype Construction for the Hybrid Necrosis Gene Ne2 and Its Distribution in Old and New Wheat Varieties. Crops, 5(3), 36. https://doi.org/10.3390/crops5030036