An Update on Root Lesion Nematode Species Infecting Cereal Crops in the Southwest of Western Australia
Abstract
1. Introduction
2. Materials and Methods
2.1. Field Sampling of Commercial Cereal Fields
2.2. Sampling of Seed Bulk-Up Nursery
2.3. Confirmation of Pratylenchus spp. Infestation
2.4. Isolation and Quantification of Pratylenchus spp. in Roots and Soil Samples
2.5. Amplification of Partial rDNA of Pratylenchus spp.
2.6. Cloning and Sequencing of Clones of D2-D3 Amplicons
2.7. Analyses of Sequences and Phylogenetic Relationships
2.8. Statistical Analysis
3. Results
3.1. Root-Lesion Nematodes Were Present in All Fields Surveyed
3.2. The Levels of Infestation in the Fields Differed Significantly
3.3. Molecular Confirmation of the Pratylenchus spp. Identified
3.4. Phylogenetic Relationships of the Pratylenchus spp. Identified, with Reference to P. curvicauda and P. quasitereoides
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sample Location | Clones Sequenced | Identified Pratylenchus spp. | Sequence Characteristics | ||||
---|---|---|---|---|---|---|---|
Unique Groups of Sequences Among the Population | Length (Nucleotides) | Positions with Insertions or Deletions | Positions with Substitutions | NCBI Genbank Accession Numbers | |||
Darkan | 26 | P. curvicauda | 7 | 312–316 | 8 | 83 | PP203078-84 |
P. penetrans | 3 | 344 | 0 | 4 | PP203085-87 | ||
Manjimup | 141 | P. penetrans | 8 | 343–344 | 3 | 11 | PP203088-95 |
P. curvicauda | 1 | 240 | NA | NA | PP203096 | ||
Cancanning | 15 | P. quasitereoides | 5 | 345 | 0 | 17 | PP203097-101 |
Katanning | 13 | P. quasitereoides | 4 | 344–346 | 2 | 12 | PP203102-105 |
Kenmare | 21 | P. penetrans | 1 | 344 | NA | NA | PP203106 |
P. quasitereoides | 2 | 344–345 | 1 | 2 | PP203107-108 | ||
Duranillin | 40 | P. penetrans | 2 | 344 | 0 | 6 | PP203109-110 |
P. quasitereoides | 1 | 192 | NA | NA | PP203111 | ||
P. neglectus | 1 | 347 | NA | NA | PP203112 |
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Copeland, R.G.R.; Iqbal, S.; Angessa, T.T.; Collins, S.J.; Jones, M.G.K.; Fosu-Nyarko, J. An Update on Root Lesion Nematode Species Infecting Cereal Crops in the Southwest of Western Australia. Crops 2025, 5, 19. https://doi.org/10.3390/crops5020019
Copeland RGR, Iqbal S, Angessa TT, Collins SJ, Jones MGK, Fosu-Nyarko J. An Update on Root Lesion Nematode Species Infecting Cereal Crops in the Southwest of Western Australia. Crops. 2025; 5(2):19. https://doi.org/10.3390/crops5020019
Chicago/Turabian StyleCopeland, Rhys G. R., Sadia Iqbal, Tefera T. Angessa, Sarah J. Collins, Michael G. K. Jones, and John Fosu-Nyarko. 2025. "An Update on Root Lesion Nematode Species Infecting Cereal Crops in the Southwest of Western Australia" Crops 5, no. 2: 19. https://doi.org/10.3390/crops5020019
APA StyleCopeland, R. G. R., Iqbal, S., Angessa, T. T., Collins, S. J., Jones, M. G. K., & Fosu-Nyarko, J. (2025). An Update on Root Lesion Nematode Species Infecting Cereal Crops in the Southwest of Western Australia. Crops, 5(2), 19. https://doi.org/10.3390/crops5020019