Next Article in Journal
Cell Culture Bioreactor Manufacturing, from Material Selection to Numerical Models
Previous Article in Journal
Dual Function WS2 Thin-Films as a Substrate for Ultrafast Response Thermocouple to Temperature Evaluation in µinjection Molding
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Materials Proceedings
Volume 8
Issue 1
10.3390/materproc2022008048
materproc-logo
Submit to this Journal
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Abstract

Gastroretentive Electrospun Nanofibers Used in Gastric Wall Wound Healing †

by
Sara F. C. Guerreiro
1,2,3,*,
Anabela G. Dias
2,
Pedro L. Granja
3 and
Juliana R. Dias
1
1
Centre for Rapid and Sustainable Product Development, Polytechnic Institute of Leiria, 2430-028 Marinha Grande, Portugal
2
Medical Physics Department, Portuguese Institute of Oncology (IPO-Porto), 4200-072 Porto, Portugal
3
Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal
*
Author to whom correspondence should be addressed.
†
Presented at the Materiais 2022, Marinha Grande, Portugal, 10–13 April 2022.
Mater. Proc. 2022, 8(1), 48; https://doi.org/10.3390/materproc2022008048
Published: 27 May 2022
(This article belongs to the Proceedings of MATERIAIS 2022)
Versions Notes

Gastric cancer is the third leading cause of death by cancer worldwide [1]. Among all the stomach cancer types, 90% correspond to gastric adenocarcinoma. In most of these epithelial tumors, a close association to Helicobacter pylori infection has been found [2]. Consequently, most of the associated therapies, namely, surgery for tumor resection and antibiotic therapy for the eradication of bacteria, usually result in the pathological healing of gastric tissues and long recovery times. This inevitably led to a significant hospitalization stay, postoperative changes in biomechanical properties of the gastric wall and difficulties in bacteria eradication [3].
Hence, the demand for innovative solutions led to the development of tissue-engineered electrospun nanofibers (eNFs) as gastric wall substitutes capable of promoting the healing of the gastric wall. In fact, eNFs with a thickness of up to 1mm provide support for cell proliferation, as well as a high surface area for the efficient delivery of proteins or antibiotic agents.
Electrospinning, being a versatile and low-cost technique, was used here to obtain an interconnected network of eNFs, composed of blended polycaprolactone (PCL)/gelatin, and crosslinked polyvinyl alcohol (PVA)/chitosan using 1,4-butanediol diglycidyl ether (BDDGE) as the crosslinking agent [4,5,6].
Hybrid eNF performance was evaluated regarding biodegradation, mucoadhesion, mechanical properties and as a drug delivery system. Considering the peristaltic movements of the stomach during the digestive process, PCL allowed to increase the tensile strength and elasticity of the whole hybrid structure. Additionally, to simulate the protein release-controlled delivery of therapeutic agents through the PCL/gelatin eNFs, protein bovine serum albumin (BSA) was successfully released with an efficiency of over 60% during the first 24 h. The inclusion of PVA/chitosan eNFs also increased the mucoadhesive properties of the membrane. Finally, the degradation profile of eNFs proved to be compatible for long-term applications (over one month).
Overall, hybrid eNFs demonstrated biodegradability and a mucoadhesive capacity, as well as promising mechanical characteristics and suitable antibiotic delivery properties to work as gastric wall substitutes.

Author Contributions

Conceptualization, S.F.C.G., A.G.D., P.L.G. and J.R.D.; methodology, S.F.C.G., A.G.D., P.L.G. and J.R.D.; validation, S.F.C.G., A.G.D., P.L.G. and J.R.D.; formal analysis, S.F.C.G., A.G.D., P.L.G. and J.R.D.; investigation, S.F.C.G., A.G.D., P.L.G. and J.R.D.; resources, S.F.C.G., A.G.D., P.L.G. and J.R.D.; data curation, S.F.C.G., A.G.D., P.L.G. and J.R.D.; writing—original draft preparation, S.F.C.G.; writing—review and editing, A.G.D., P.L.G. and J.R.D.; visualization, S.F.C.G., A.G.D., P.L.G. and J.R.D.; supervision, A.G.D., P.L.G. and J.R.D.; project administration, A.G.D., P.L.G. and J.R.D.; funding acquisition, A.G.D., P.L.G. and J.R.D. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Fundação para a Ciência e Tecnologia (FCT) grant number 2021.05893.BD, UIDB/04044/2020 and UIDP/04044/2020. This study was also supported by PAMI-ROTEIRO/0328/2013 (Nº 022158), MATIS (CENTRO-01-0145-FEDER-000014-3362).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Rawla, P.; Barsouk, A. Epidemiology of gastric cancer: Global trends, risk factors and prevention. Gastroenterology Rev. 2019, 14, 26–38. [Google Scholar] [CrossRef] [PubMed]
  2. Alipour, M. Molecular Mechanism of Helicobacter pylori-Induced Gastric Cancer. J. Gastrointest. Cancer 2021, 52, 23–30. [Google Scholar] [CrossRef] [PubMed]
  3. Ajani, J.A.; Lee, J.; Sano, T.; Janjigian, Y.Y.; Fan, D.; Song, S. Gastric adenocarcinoma. Nat. Rev. Dis. Primers 2017, 3, 1–19. [Google Scholar] [CrossRef] [PubMed]
  4. Guerreiro, S.F.C.; Valente, J.F.A.; Dias, J.R.; Alves, N. Box-Behnken Design a Key Tool to Achieve Optimized PCL/Gelatin Electrospun Mesh. Macromol. Mater. Eng. 2021, 306, 2000678. [Google Scholar] [CrossRef]
  5. Juliana, D.; Pedro, G.; Paulo, B. Internal crosslinking evaluation of gelatin electrospinning fibers with 1,4 butanediol Diglycidyl ether (Bddge) for skin regeneration. In Proceedings of the 10th World Biomaterials Congress, Montréal, Canada, May 17–22 2016; Frontiers in Bioengineering and Biotechnology: Lausanne, Switzerland, 2016. [Google Scholar] [CrossRef]
  6. Dias, J.R.; dos Santos, C.; Horta, J.; Granja, P.L.; Bártolo, P.J. A new design of an electrospinning apparatus for tissue engineering applications. Int. J. Bioprinting 2017, 3, 121–129. [Google Scholar] [CrossRef] [PubMed]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Guerreiro, S.F.C.; Dias, A.G.; Granja, P.L.; Dias, J.R. Gastroretentive Electrospun Nanofibers Used in Gastric Wall Wound Healing. Mater. Proc. 2022, 8, 48. https://doi.org/10.3390/materproc2022008048

AMA Style

Guerreiro SFC, Dias AG, Granja PL, Dias JR. Gastroretentive Electrospun Nanofibers Used in Gastric Wall Wound Healing. Materials Proceedings. 2022; 8(1):48. https://doi.org/10.3390/materproc2022008048

Chicago/Turabian Style

Guerreiro, Sara F. C., Anabela G. Dias, Pedro L. Granja, and Juliana R. Dias. 2022. "Gastroretentive Electrospun Nanofibers Used in Gastric Wall Wound Healing" Materials Proceedings 8, no. 1: 48. https://doi.org/10.3390/materproc2022008048

APA Style

Guerreiro, S. F. C., Dias, A. G., Granja, P. L., & Dias, J. R. (2022). Gastroretentive Electrospun Nanofibers Used in Gastric Wall Wound Healing. Materials Proceedings, 8(1), 48. https://doi.org/10.3390/materproc2022008048

Article Metrics

Back to TopTop