New Advances in Fibroblast Growth Factor-Based Coatings for Hip Replacement Implants †
1
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
2
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
3
Research Institute of the University of Bucharest-ICUB, 91-95 Splaiul Independentei, 050095 Bucharest, Romania
4
National Institute for Lasers, Plasma and Radiation Physics, Măgurele 077125, Romania
5
Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1–3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania
*
Author to whom correspondence should be addressed.
†
Presented at the 2nd Coatings and Interfaces Web Conference, 15–31 May 2020; Available online: https://ciwc2020.sciforum.net/.
Mater. Proc. 2020, 2(1), 9; https://doi.org/10.3390/CIWC2020-06844
Published: 15 May 2020
(This article belongs to the Proceedings of 2nd Coatings and Interfaces Web Conference (CIWC-2 2020))
It is already well known that the tissue–implant interface is one of the most critical factors for the success of implant integration. The use of bioactive and biomimetic surfaces is of great interest in biomedical applications, especially in tissue engineering. Therefore, in our study, we aimed to obtain successful coatings based on hydroxyapatite, antibiotics and growth factors in order to increase the biocompatibility of commercial implant materials by promoting cell attachment and growth without toxic effects, as well as the inhibition of microbial biofilm formation. In this way, homogenous mixtures of hydroxyapatite, kanamycin and fibroblast growth factor (HAP/KAN, HAP/FGF and HAP/KAN/FGF) were coated on titanium-based metal plates for hip replacement implants. The coatings were able to impair the initial adherence of bacterial cells and to reduce biofilm formation throughout the release of antibiotics. The cytocompatibility of these samples was investigated on normal murine osteoblasts (MC3T3-E1 cell line) with fibroblast-like morphologies by evaluating their influence on cellular viability and their potential to generate an inflammatory response. In addition, adhesion and proliferation, as well as actin cytoskeleton organization, were observed after 24 h of cell culture on these coatings. The results confirmed the biocompatibility of all coatings, with the cell number counted for the HAP/KAN/FGF sample being equal to the control. Since it is well known that NO is a marker of inflammation with an essential role in regulating apoptotic cell death and cell viability, our study showed that cell growth on these surfaces did not induce nitric oxide (NO) release, with the NO level being maintained close to control values for all tested samples. Moreover, an excellent cell adherence and spreading on these coatings deposited on hip implants was evidenced by fluorescence microscopy, supporting their usage as substrates in tissue engineering applications.
Keywords:
biocoatings; fibroblast growth factor; hydroxyapatite; kanamycin; hip implants
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Nica, I.C.; Stan, M.S.; Dinischiotu, A.; Grumezescu, V.; Stoica, A.E.; Holban, A.; Grumezescu, A.M. New Advances in Fibroblast Growth Factor-Based Coatings for Hip Replacement Implants. Mater. Proc. 2020, 2, 9. https://doi.org/10.3390/CIWC2020-06844
AMA Style
Nica IC, Stan MS, Dinischiotu A, Grumezescu V, Stoica AE, Holban A, Grumezescu AM. New Advances in Fibroblast Growth Factor-Based Coatings for Hip Replacement Implants. Materials Proceedings. 2020; 2(1):9. https://doi.org/10.3390/CIWC2020-06844
Chicago/Turabian StyleNica, Ionela Cristina, Miruna S. Stan, Anca Dinischiotu, Valentina Grumezescu, Alexandra Elena Stoica, Alina Holban, and Alexandru Mihai Grumezescu. 2020. "New Advances in Fibroblast Growth Factor-Based Coatings for Hip Replacement Implants" Materials Proceedings 2, no. 1: 9. https://doi.org/10.3390/CIWC2020-06844
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
