Advanced Characterization and Sample Preparation Strategies for Nanoformulations
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThe authors presented the paper "Advanced Characterization and Sample Preparation Strategies for Nanoformulations"
1) The reference list should be improved. I see a lot of old references and not too many within the last 5 years.
2) DLS. Please, Present some results (pictures) of DLS in number, volume, and intensity modes and explain what these modes show.
The instrument accurately determined particles in the size range of 10-100nm. - The instrument shows the sizes from 10 to 10000. The new ones can show the concentration of nanoparticles. Please, update the data from the DLS instrument websites.
3) Can you present each method with some pictures with interesting results? These figures should provide information about what method can be useful.
4) One problem with the methods should be noted. The DLS shows the sizes with the hydration sphere which is why the sizes may be not the same as for TEM and SEM. Some good examples should be presented about it.
5) Moreover, one good example of nanoparticle aggregation under TEM conditions may be inserted. This means that under sample preparation aggregation may occur and the data will be not comparable with DLS.
6) Can you summarize the methods' advantages, disadvantages, resolution, what they show, etc. in Table.
Comments on the Quality of English LanguageMinor editing of English language required
Author Response
- The reference list should be improved. I see a lot of old references and not too many within the last 5 years.
Ans: As per the suggestion, the reference list has been updated.
- Please, Present some results (pictures) of DLS in number, volume, and intensity modes and explain what these modes show.
Ans: We acknowledged the reviewer's comments and also the importance of understanding what is this mode and when to use it. Our article primarily focuses on sample preparation which is one of the important parameters for the measurement but still looking importance of this, we added one separate section in the manuscript and highlighted it in yellow color.
- The instrument accurately determined particles in the size range of 10-100nm. - The instrument shows the sizes from 10 to 10000. The new ones can show the concentration of nanoparticles. Please, update the data from the DLS instrument websites.
Ans: Required changes have been made in the revised manuscript.
- Can you present each method with some pictures with interesting results? These figures should provide information about what method can be useful.
Ans: We added the needful image as recommended.
- One problem with the methods should be noted. The DLS shows the sizes with the hydration sphere which is why the sizes may be not the same as for TEM and SEM. Some good examples should be presented about it.
Ans: Thank you for the suggestion, the very interesting example has been included in the revised manuscript.
- Moreover, one good example of nanoparticle aggregation under TEM conditions may be inserted. This means that under sample preparation aggregation may occur and the data will be not comparable with DLS.
Ans: We appreciate the comment. We have added content to the revised manuscript. However, it is unable to find the relevant study in the literature to add a diagram to the revised manuscript.
- Can you summarize the methods' advantages, disadvantages, resolution, what they show, etc. in Table.
Ans: As suggested, a summary of all techniques has been included in the revised manuscript.
Reviewer 2 Report
Comments and Suggestions for AuthorsTo improve your manuscript as requested, consider the following comments:
1. Improve Image Quality for All Figures:
- Enhance the resolution and clarity of all images, like in Figure 4.
2. Add Improved Diagrams:
- Create and insert detailed diagrams illustrating the principles and working mechanisms of
3. Add Subheading for Principles and Uses: Create a subheading titled Principles and Uses of Microscopy Techniques and provide detailed explanations of LM, TEM, and SEM under this section.
4. Illustrate Clear Diagrams and Comparisons:
- Design clear diagrams for each technique, possibly including real images to better illustrate the practical applications and results of LM, TEM, and SEM.
- Compare closely related techniques if applicable.
5. Ensure All 99 References Are Cited within context:
- Review the manuscript to ensure that all 99 references listed are properly cited in the text.
Author Response
- Improve Image Quality for All Figures:
- Enhance the resolution and clarity of all images, like in Figure 4.
Ans: All images have been verified for resolution and clarity. Image 3 has been updated in the revised manuscript.
- Add Improved Diagrams:
- Create and insert detailed diagrams illustrating the principles and working mechanisms of
Ans: Thank you for your comment, few diagrams have been added in the revised manuscript.
- Add Subheading for Principles and Uses: Create a subheading titled Principles and Uses of Microscopy Techniques and provide detailed explanations of LM, TEM, and SEM under this section.
Ans: We appreciate the reviewer’s points; however, subheadings have been given as per the journal's guidelines.
- Illustrate Clear Diagrams and Comparisons:
- Design clear diagrams for each technique, possibly including real images to better illustrate the practical applications and results of LM, TEM, and SEM.
- Compare closely related techniques if applicable.
Ans: Thank you for the suggestions, we have newly added diagrams for practical applications of techniques.
- Ensure All 99 References Are Cited within context:
- Review the manuscript to ensure that all 99 references listed are properly cited in the text.
Ans: The whole manuscript has been checked for all the references.
Round 2
Reviewer 1 Report
Comments and Suggestions for AuthorsThank you for the revised paper.
line 184 HAuCl4
Comments on the Quality of English LanguageMinor editing of English language required.