3.2.1. Results of the Feed-Meter Calibration
The calibration results revealed a strong linear correlation between the programmed and actual stepper motor speeds up to 164 rpm, beyond which deviations became more pronounced (
Figure 7). This finding indicates reliable and predictable motor control within the 30–164 rpm range, which is critical for ensuring consistent feed output in automated systems. The findings also highlight the torque limitations of the NEMA 17 motor at higher speeds, where diminished output accuracy may impact dispensing precision. These results support the system’s operational suitability within the identified speed range, aligning with design objectives for accurate and efficient feed metering in aquaculture applications [
14].
Beyond 164 rpm, the speed decreases sharply, indicating the system’s mechanical or electrical saturation point due to insufficient motor torque or increased friction [
15].
Figure 8 shows that the percent speed deviation remained minimal up to 164 rpm but spiked to 82.22%, confirming control instability and torque transfer inefficiency.
The likely cause is the inherent torque drop-off of the NEMA 17 motor at high speeds and load resistance, resulting in skipped steps or stalled operations. Thus, 164 rpm represents the practical upper limit for reliable feed metering.
The calibration curve (
Figure 9) yielded an R
2 value of 0.999, indicating a strong linear fit between auger revolutions and the feed mass delivered. This corresponds to a consistent feed rate of 20.883 g per revolution, enabling the control system to translate target feed masses into auger rotations accurately. At 163 rpm, the system delivered 100 g of feed in approximately 1.70 s, confirming its fast and precise dispensing capability with minimal error.
The established calibration curve is critical for ensuring precise and programmable feed delivery. The system eliminates assumptions by defining a fixed, linear relationship between auger revolutions and feed mass and enables consistent, repeatable feeding across multiple operations. This allows the automatic feeder to deliver accurate quantities based on user-defined input, supporting effective feed management, minimizing waste, and enhancing operational reliability in aquaculture applications.
3.2.2. Results of the Feed Dispersing Performance Test
Before modeling, Statistical analysis using a Box–Behnken design assessed the feeder’s performance across three input variables. Mean Radius Spread and High Concentration Area satisfied normality assumptions (
p = 0.3425 and
p = 0.8157, respectively), while Total Spread Area did not (
p = 0.0142), indicating the need for alternative analytical approaches for that response in future studies (
Table 3).
Table 4 presents the experimental results obtained from the three-factor BBD, where the Average Radius Spread (cm) served as the response variable. The table summarizes the observed values across 15 experimental runs, reflecting the combined effects of impeller speed (
), feed mass per cycle (
), and time of spread (
) on feed dispersion.
Table 4 summarizes the experimental results of the three-factor BBD, with average radius spread (
Y) as the response variable. Results showed that both impeller speed (
) and feed mass per cycle (
) had significant effects on feed distribution, while spreading time (
) contributed moderately. Increasing impeller speed and feed mass improved dispersion up to optimal levels, beyond which turbulence reduced uniformity. These findings are consistent with previous studies reporting that vortex-type impellers generate higher flow pressure and improved mixing efficiency [
16], and that centrifugal impeller systems can achieve effective feed dispersion under controlled propulsion conditions [
17]. Optimal operating conditions were achieved at moderate combinations of
and
, resulting in consistent radial feed distribution under stable water flow and aeration. This result supports the system’s mechanical feasibility and its potential to deliver uniform, bottom-directed feed dispersion for shrimp aquaculture.
3.2.3. Regression and Analysis of Variance of the Experiment Results
An ordinary least squares (OLS) multiple linear regression model was used to assess the effects of rotational speed (rpm), mass, and time, including their quadratic and interaction terms, on the mean radius spread. The regression coefficients and associated analysis of variance (ANOVA) results are summarized in
Table 5. The model demonstrated a strong fit, with an R
2 value of 0.919, indicating that approximately 91.9% of the variability in the mean radius spread was explained by the predictors. The adjusted R
2 of 0.773 provides a more conservative evaluation of model performance by accounting for model complexity relative to the limited sample size (
n = 15).
To formally assess model adequacy beyond goodness-of-fit statistics, a lack-of-fit test was conducted using replicated center points from the Box–Behnken design. The results of this analysis are presented in
Table 6. The lack-of-fit test yielded an F-statistic of 11.74 with a corresponding
p-value of 0.0795, which exceeds the significance threshold of
. This indicates that the lack of fit is not statistically significant, confirming that the quadratic regression model sufficiently represents the experimental response within the investigated design space.
The F statistic of 6.300 and a
p value of 0.0283 confirm the model’s overall significance at the 5% level, indicating that at least one predictor meaningfully helps explain the response variable. The regression model offers quantitative insights into how rotational speed, mass, and time, along with their interactions, influence feed particle dispersion, as detailed in the coefficient estimates and
p values presented in
Table 7.
The multiple linear regression results revealed that the intercept (124.10,
p = 0.004) was statistically significant, serving as a baseline for interpreting predictor effects, although it lacks practical meaning. Among the predictors, rotational speed was the only significant linear term (
p = 0.011) with a negative coefficient (−0.3322), indicating that higher impeller speeds reduce the mean spread width. This reduction is attributed to vortex formation at elevated speeds, which pulls particles inward and limits radial dispersion, a finding that is consistent with previous fluid dynamics studies [
18].
The quadratic term for rotational speed (
p = 0.005, coefficient = 0.0004) suggests a U-shaped relationship where the spread radius decreases but increases again beyond a critical speed, reflecting the balance between centrifugal forces and vortex-induced inward drag. The feed mass had no significant effect on the spread width (
p = 0.591), which aligns with research indicating that fluid flow dynamics, rather than mass alone, dominate dispersion behavior [
19].
Time exhibited a negative but marginally nonsignificant effect (
p = 0.074), suggesting that prolonged operation may reduce the spread width due to vortex development beneath the impeller, which is consistent with existing research on flow disruption in pumps [
13,
16]. All interaction terms (rotational speed × mass, rotational speed × time, and mass × time) were nonsignificant, suggesting that these variables act independently within the tested ranges. Overall, rotational speed emerged as the primary driver of feed spread behavior, emphasizing the need to calibrate optimal speed settings to balance dispersion and vortex control.
where:
Y—is the predicted response of the mean radius spread;
β0—is the intercept of the model;
X1—is the rotational speed of the impeller (rpm);
X2—is the mass of the feed (g);
X3—is the time of spread (s);
β1—is the coefficient of the revolutions per minute of the impeller;
β2—is the coefficient of mass of the feed;
β3—is the coefficient of the time of spread.
The regression model demonstrates a statistically significant nonlinear (quadratic) relationship between impeller rotational speed (rpm) and the radial spread of feed particles. Specifically, the negative linear coefficient (β1 = −0.3322) indicates that, at lower to moderate rpm levels, an increase in rotational speed results in a reduction in mean spread radius. In contrast, the positive quadratic coefficient (β11 = 0.0004) reflects a reversal in this trend beyond a critical rotational speed threshold, where further increases in rpm are associated with an expansion of the radial spread. This results in a concave upward response surface, characteristic of a parabolic turning point.
These findings are consistent with established fluid dynamics principles. At moderate rotational speeds, impeller-induced vortical structures may develop, generating inward forces that diminish outward dispersion efficiency. As the rotational speed surpasses this critical threshold, the kinetic energy imparted to the fluid becomes sufficient to counteract these centripetal effects, thereby enhancing the radial dispersion of feed particles. Nonetheless, excessively high rotational speeds are likely to promote turbulence and energy dissipation, which are not fully accounted for within the constraints of the second-order polynomial model. This limitation suggests the need for complementary computational fluid dynamics (CFD) analyses to capture the system’s complex flow behaviors comprehensively.
The model’s intercept term (β0 = 124.0996) serves as a statistical baseline, representing the predicted mean spread radius when all predictors are set to zero. However, this condition lacks practical relevance, as zero rotational speed, mass, and time inherently imply the absence of feed dispersion. While feed mass and spread time exhibited lower magnitude and statistically non-significant effects within the tested parameter ranges, their inclusion in the model provides additional capacity to detect higher-order or interaction effects that may arise under varying operational conditions. Collectively, the results confirm impeller rotational speed as the primary determinant of feed dispersion performance, with the quadratic model effectively capturing the system’s nonlinear and non-monotonic response patterns, which would otherwise remain undetected in a purely linear modeling framework.
3.2.4. Surface Response
Three surface response plots were generated using the Box–Behnken experimental design data to better understand how the input variables influenced feed dispersion. All statistical analyses were conducted in Jupyter Notebook (Python 3.11) using the pandas, numpy, matplotlib, statsmodels, and scipy libraries. A second-order polynomial model was fitted using the ordinary least squares (OLS) method implemented in the statsmodels package, while model assumptions such as normality and homogeneity of variance were verified using the Shapiro and Levene tests, respectively. The resulting three-dimensional surface plots were visualized with matplotlib. The interactions between rotational speed, 285.98 rpm (low), 440.74 rpm (mid), and 586.85 rpm (high), and feed mass, 95.23 g, 190.45 g, and 285.68 g, were examined relative to the mean radius spread of feed, with each plot constructed at fixed spread durations of 2, 5, and 8 s. Across all conditions, rotational speed exerted the most significant influence on feed dispersion, as illustrated in
Figure 10.
At 2 s (
Figure 10a), maximum spread exceeds 55 cm, achieved at low-to-mid rpm and moderate feed mass, indicating efficient dispersion before fluid instabilities develop. High rpm settings already show a slight performance reduction, suggesting early vortex formation, which restricts outward feed movement. The surface reveals a broad, concave optimal zone centered at mid-range rpm, where centrifugal forces efficiently distribute the feed.
By 5 s (
Figure 10b), the maximum spread slightly declines to ~52.5 cm, with noticeable performance drops at high rpm across all feed masses. The optimal operating zone narrows and shifts toward lower rpm, implying that vortex formation intensifies with time, undermining dispersion at higher speeds. This reflects the model’s quadratic findings, confirming that dispersion efficiency declines beyond a certain rpm threshold.
At 8 s (
Figure 10c), dispersion patterns become more restricted, with optimal spread (~55 cm) confined to low rpm and feed mass. Both high rpm and feed mass significantly reduce spread, highlighting the progressive formation of stable vortex structures. These vortices draw particles inward, counteracting centrifugal dispersion and limiting feed coverage in the tank’s outer regions.
The overall trend shows that while moderate rpm and feed mass maximize early dispersion, prolonged operation requires progressively lower rpm settings to maintain effectiveness. Operating at excessive speeds over time enhances vortex-induced inward flow, reducing feed spread efficiency.
These results emphasize the need for dynamic rotational speed adjustment based on operating time to optimize feed dispersion and minimize waste in aquaculture systems. Maintaining the system within an efficient, vortex-minimized flow regime is essential for consistent feed coverage.
3.2.5. Cross-Validation of the Response Surface Model
Leave-one-out cross-validation (LOOCV) was conducted as an internal robustness check for the quadratic response surface model due to the limited number of experimental runs inherent to the Box–Behnken design. LOOCV is commonly applied to small-scale, structured experimental datasets to assess model stability and generalizability when independent validation experiments are not feasible [
20]. In the context of response surface methodology, LOOCV has been shown to be particularly useful for evaluating local predictive behavior within the design space rather than for establishing global predictive performance [
21].
Figure 11 illustrates the relationship between observed mean radius spread values and LOOCV-predicted responses. The dashed line represents the line of equality (y = x), where predicted values perfectly match observed values; points lying closer to this line indicate higher predictive accuracy. Predictions show closer agreement near the center of the design space, while larger deviations occur at boundary points, consistent with the expected behavior of response surface models derived from Box–Behnken designs.
The LOOCV analysis yielded a predicted of −1.03, compared with an adjusted of 0.665 for the fitted model, indicating limited predictive accuracy for individual unseen runs. A negative predicted implies that, when predicting unseen observations, the model performs worse than simply using the overall mean response. This outcome does not indicate that the response surface model is ineffective; rather, it reflects weak predictive robustness for individual runs, which is commonly observed in Box–Behnken designs with very limited experimental runs. Importantly, although optimization results are often visualized or discussed in terms of a single factor (e.g., impeller rotational speed), the fitted response surface is inherently multivariate, as it was developed from simultaneous variations in rotational speed, feed mass, and dispersion time. Consequently, the response cannot be fully expressed or predicted as a univariate function of rotational speed alone without specifying the remaining parameters. Analysis of the prediction residual sum of squares (PRESS = 1843.69) further revealed that approximately 86% of the total prediction error originated from a small number of runs located at the boundaries of the experimental domain, while runs near the center exhibited relatively small errors. This behavior highlights the local and multivariate nature of quadratic response surface models, which provide the most reliable predictions within regions of high data density and increased uncertainty at extreme combinations of the design variables.
Characterization analysis of the quadratic response surface model was conducted to describe the nonlinear relationship between impeller rotational speed and feed dispersion behavior within the Box–Behnken design space, with model reliability first assessed through LOOCV. As shown in
Figure 11, predictive agreement is strongest near the center of the experimental domain, where data density is highest, while increased deviations occur at boundary conditions. Within this validated context, analysis of the fitted model identified an operating point at approximately 415 rpm, corresponding to a predicted mean radius spread of 31.59 cm. This speed was selected because it lies near the central region of the response surface, where the Box–Behnken design provides balanced factor variation and reduced estimation variance compared with boundary points. To account for model uncertainty, the estimated response was accompanied by a 95% confidence interval (26.16–37.02 cm) and a 95% prediction interval (20.70–42.49 cm), representing uncertainty in the estimated mean and the expected variability of individual experimental outcomes, respectively. The results indicate that feed dispersion improves with increasing rotational speed up to a threshold, beyond which further increases in speed or prolonged dispersion duration promote the formation of a strong central vortex. This vortex induces localized recirculation and vertical flow dominance, which negatively affects radial feed transport and reduces dispersion uniformity. Accordingly, the identified operating point should be interpreted as an efficient condition within the central region of the investigated experimental domain, where hydrodynamic behavior remains stable and model predictions are most reliable, rather than a globally optimal setting applicable beyond the tested parameter space.
3.2.6. Coefficient of Variation (CV) from Image-Based Spread Analysis
Image analysis via OpenCV in Python was applied to evaluate feed dispersion consistency through the coefficient of variation (CV), which quantifies spatial variability by comparing high-concentration feed zones to the total dispersed area. The visual outputs included binary masks, grayscale intensity plots, and heatmaps, offering insight into dispersion patterns (
Figure 12).
The results revealed a central feed concentration with a limited radial reach, which was likely due to the impeller geometry and insufficient outward velocity. At higher RPMs, vortex formation and centripetal flow may trap feed centrally, whereas physical feed properties, such as pellet density and moisture content, further limit dispersion due to rapid settling. The feed density heatmap overlay in
Figure 12 shows this distribution pattern, where warmer colors (yellow to red) indicate higher feed concentration and cooler colors (blue to green) represent lower feed density across the tank surface. These findings are consistent with the surface response results, indicating a reduced mean spread at high speeds.
The lowest CV (0.13) occurred in Run 4 (low RPM, middle mass, high time), suggesting that a longer dispersion time and lower impeller speed promote stable flow fields and more uniform feed spread. High-speed trials (CV = 0.21–0.25) resulted in a localized but consistent distribution, whereas mid-speed, low-mass/time runs (CV = 0.32–0.35) presented greater variability, highlighting the sensitivity of feed spread to interaction effects.