Next Article in Journal
Gamification Concepts to Promote and Maintain Therapy Adherence in Children with Growth Hormone Deficiency
Previous Article in Journal
Good Things in Small Packages? Evaluating an Economy of Scale Approach to Behavioral Health Promotion in Rural America
Article Menu

Export Article

Open AccessArticle
J 2018, 1(1), 57-70; https://doi.org/10.3390/j1010007

Pathway Analysis of a Transcriptome and Metabolite Profile to Elucidate a Compensatory Mechanism for Taurine Deficiency in the Heart of Taurine Transporter Knockout Mice

1
Faculty of Biotechnology, Fukui Prefectural University, Fukui 910-1195, Japan
2
College of Medicine, University of South Alabama, Mobile, AL 36688, USA
*
Author to whom correspondence should be addressed.
Received: 25 July 2018 / Revised: 20 August 2018 / Accepted: 25 August 2018 / Published: 27 August 2018
Full-Text   |   PDF [2788 KB, uploaded 27 August 2018]   |  
  |   Review Reports

Abstract

Taurine, which is abundant in mammalian tissues, especially in the heart, is essential for cellular osmoregulation. We previously reported that taurine deficiency leads to changes in the levels of several metabolites, suggesting that alterations in those metabolites might compensate in part for tissue taurine loss, a process that would be important in maintaining cardiac homeostasis. In this study, we investigated the molecular basis for changes in the metabolite profile of a taurine-deficient heart using pathway analysis based on the transcriptome and metabolome profile in the hearts of taurine transporter knockout mice (TauTKO mice), which have been reported by us. First, the genes associated with transport activity, such as the solute carrier (SLC) family, are increased in TauTKO mice, while the established transporters for metabolites that are elevated in the TauTKO heart, such as betaine and carnitine, are not altered by taurine deficiency. Second, the integrated analysis using transcriptome and metabolome data revealed significant increases and/or decreases in the genes involved in Arginine metabolism, Ketone body degradation, Glycerophospholipid metabolism, and Fatty acid metabolism in the KEGG pathway database. In conclusion, these pathway analyses revealed genetic compensatory mechanisms involved in the control of the metabolome profile of the taurine-deficient heart. View Full-Text
Keywords: taurine; osmoregulation; metabolomics; transcriptomics; pathway analysis taurine; osmoregulation; metabolomics; transcriptomics; pathway analysis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Ito, T.; Murakami, S.; Schaffer, S. Pathway Analysis of a Transcriptome and Metabolite Profile to Elucidate a Compensatory Mechanism for Taurine Deficiency in the Heart of Taurine Transporter Knockout Mice. J 2018, 1, 57-70.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J EISSN 2571-8800 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top