1. Introduction
Sustainable soil management is essential to conserve biodiversity, and the optimization of its physical, chemical, and biological properties ensures its productivity and ecosystem functionality. Soil degradation is a global reality, intensified by unsustainable agricultural practices, deforestation, and the expansion of aggressive anthropogenic activities that alter the vegetation cover and exacerbate the effects of climate change [
1,
2]. Uncontrolled agricultural activities are recognized as the main factor in soil degradation [
3]. It is estimated that 33% of the world’s land is moderately or severely degraded, and an additional 44% faces risks of erosion, acidification, compaction, and chemical contamination [
4].
In Latin America, this situation is particularly critical, as the intensification of agriculture and the excessive use of agrochemicals have seriously compromised the soil quality, affecting its structure and reducing its productivity in the long term [
5]. On the other hand, since agrochemical residues persist in biodiversity, they end up altering food chains and posing risks to both the environment and public health [
6].
Faced with this problem, the use of biofertilizers is becoming established as a promising and viable ecological alternative to mitigate the negative effects of soil degradation, since these products improve the chemical–physical and biological properties of the soil, such as aeration and the water retention capacity. They also increase the availability of essential nutrients for plants and promote microbial activity, which is essential for the decomposition of organic matter and the release of nutrients [
7,
8,
9].
In this context,
Swietenia macrophylla (Mahogany) and
Artocarpus heterophyllus (Yaca) are species of great ecological and economic value in tropical ecosystems. Mahogany, in addition to its high demand in the timber industry, plays a crucial role in carbon capture and the restoration of degraded forests [
10]. This species is classified as vulnerable by the International Union for Conservation of Nature (IUCN) because it suffers from intense deforestation [
11]. On the other hand, the Yaca is a fruit tree of great economic and food importance in various tropical regions [
12], and both it and Mahogany are species that are commonly used in the restoration and sustainability of forest ecosystems [
12,
13,
14].
Despite the growing interest in the use of biofertilizers, their impact on forest species in the Central Forest of Peru remains little explored. This region is of the utmost importance given that it is part of the Amazon, an area that is highly sensitive to activities such as deforestation, which bring unpredictable global consequences and a strong impact on processes such as climate change.
In this work, the effects of biofertilizers such as biol, vermicompost, and bokashi on the physical, chemical, and biological properties of the soil and its relationship with the vegetative development of both Yaca and Mahogany are evaluated. These biofertilizers were chosen taking into account that they not only improve the vegetative growth of plants but also contribute to their resistance to diseases and their adaptation to adverse climatic conditions [
15,
16]. We believe that the results obtained will provide important information for sustainable soil management and the conservation of forest species in tropical ecosystems, contributing to reforestation strategies and the mitigation of soil degradation.
2. Materials and Methods
2.1. Study Site
This work was carried out at Villa Ashaninka (Pichanaqui-Chanchamayo) in the Junin region in the Central Peruvian Jungle (10°55′35” S; 74°52′22” W; 525 m.a.s.l). This region is characterized by a warm, humid, and rainy equatorial climate throughout the year, where the average annual temperature is between 21 °C and 33 °C, with minimum values above 19 °C and maximum values below 35 °C. The average annual rainfall is 458 mm, with average humidity of 65% and a UV index of 5 [
17].
2.2. Preparation of Biofertilizers
An area of 6 m × 8 m was prepared for the production of each of the biofertilizers, biol (T02) [
18,
19], vermicompost (T03), and bokashi (T04) [
20,
21]. We labeled the control treatment without biofertilizer as T01.
T02 was prepared in a biodigester in which 10 kg of sheep manure, 4 kg of brown sugar, 5 kg of phosphate rock, 6 kg of ash, 3 kg of lime, 1 kg of ground eggshells, 5 kg of kudzu leaves, 250 g of fresh yeast, and enough water to cover three-quarters of the biodigester were added, along with 3 L of “guarapo” (juice from pressed sugar cane). The mixture was fermented for 50 days.
T03 was a commercial organic fertilizer (worm humus) purchased from the company Lombri-Humus Peru [
22,
23].
T04 was prepared from inputs such as 200 kg of sheep manure, 100 kg of ash, 500 kg of agricultural soil, 1000 kg of organic waste, 5 kg of ground eggshells, 35 kg of lime, 5 kg of sugar, 250 g of dry yeast, effective microorganism (EM) compost [
24], and 400 L of irrigation water. All components were mixed homogeneously and the mixture was sprayed with a solution of sugar and EM compost. The mixture was then sealed with plastic to promote fermentation for 15 days, reaching temperatures of up to 42 °C. Weekly turning was performed, and, after 45 days, T04 was harvested.
2.3. Nursery Phase
The nursery was established taking into account the solar orientation and wind patterns. Seedbeds measuring 1 m × 10 m were constructed, on which a layer of washed and fungicide-disinfected sand was applied. After irrigation, holes were created in the sand to sow seeds of Yaca (Artocarpus heterophyllus) and Mahogany (Swietenia macrophylla), which were then covered with a thin layer of sand.
Once the seedlings reached four leaves, the most vigorous individuals were selected to be transplanted one by one into bags with substrate mixtures composed of agricultural soil, decomposed organic matter, and sand in a ratio of 2:1:1. Humidity was maintained by sprinkler irrigation, especially during the late afternoon hours.
2.4. Plant Placement in the Final Field and Fertilization
After five months in the nursery, the Mahogany and Yaca seedlings were transplanted to the final field. Each plant was labeled with the same nomenclature as for the biofertilizer used, i.e., T04 for the bokashi treatment, T03 for the vermicompost treatment, T02 for the biol treatment, and T01 for the control treatment without any biofertilizer.
At 15 days after planting, the biofertilizers T04 (5 kg per hole), T02 (10 L mixed with 10 L of water), and T03 (5 kg per hole) were applied, following the recommendations of [
25].
2.5. Sampling of Biofertilizers and Soils Before and After Fertilization
2.5.1. Sampling of Biofertilizers
To determine the quality and composition of the biofertilizers, 1 L samples of T02 and 1 kg of both T04 and T03 were taken. The physical–chemical and microbiological analyses were carried out in the laboratory of the Foundation for Agrarian Development of the Agrarian University La Molina.
2.5.2. Sampling of Soil Before (M.I.) and After Fertilization
To ensure the representativeness of the soil samples before and after fertilization, subsamples were collected at different points in the plot. After mixing and dividing them, representative samples of 1 kg were obtained, which were subjected to physical–chemical and biological analyses.
2.6. Evaluation of Biometric Parameters
To collect data on both the height and the number of leaves of the plants, 8 samples were randomly selected for each treatment and measurements were recorded at 30, 60, and 120 days after fertilization.
2.7. Evaluation of the Presence of Vegetation and Soil Fauna After the Incorporation of Biofertilizers
After 5 months of treatment with the biofertilizers, the vegetation was evaluated using an observation sheet. Soil samples were taken in an area of 20 cm2 to identify plant species (common name, scientific name, family) and measure the lengths of their roots. In the same samples, the presence of soil fauna (insects, arachnids, annelids, etc.) was recorded and evaluated using a magnifying glass.
2.8. Chlorophyll Determination
The chlorophyll content was determined using the spectrophotometric method. Leaves (8 to 10) were collected from three-quarters of the height of the plants subjected to each treatment. These leaves were ground in a mortar and ethanol (96%) was added; then, aliquots of the solutions were filtered and transferred to 10 mL cuvettes, which were analyzed, using a UV SQ2802 spectrophotometer, in terms of absorbance at wavelengths of 480, 649, and 665 nm. The absorbance values obtained were used to calculate the chlorophyll A, B and total chlorophyll content, applying the equation proposed by [
26].
2.9. Statistical Analysis
For data analysis, a database was created in a spreadsheet and subsequently processed using the RStudio (version 4.3.3) and Past 4.11 software. The statistical analysis included an analysis of variance (ANOVA) to assess the presence of significant differences among the studied variables, as well as Tukey’s multiple comparison test to determine differences between treatments. Additionally, the Shannon index was calculated to evaluate the diversity of soil fauna and vegetation. Variables such as the plant height, number of leaves, and chlorophyll concentration were analyzed. The results were expressed in terms of the probability value (p-value) and represented through graphs generated using the employed software.
3. Results
3.1. Effects of Biofertilizers on Soil Physical Characteristics
Figure 1 shows the physical properties of the soil before and after fertilization. The use of biofertilizers caused a significant decrease in the apparent density of the soil (see
Figure 1a), particularly in treatments T03 and T04, decreasing in the latter by up to 15% with respect to T01. Consequently, as expected, the porosity (
Figure 1b) increased significantly with T04 (~57%). This soil could be described as a highly porous or less dense soil. On the other hand, it could be seen that the porosity with T02 was highly similar to that of T01 (~52%).
The water retention capacity (WRC) of the soil (see
Figure 1c) was also positively influenced by the treatments and especially the application of the biofertilizers. Thus, with T03, we obtained the highest WRC value (almost 25%), whereas T02 and T04 had comparable values, at ~1% less than T03 but ~2% more than T01.
3.2. Effects of Biofertilizers on Soil Chemical Characteristics
Before the application of the biofertilizers (M.I.), the soil had a slightly acidic pH, with a value of 6.23. The use of treatment T01 led to almost no variation in this parameter; in contrast, treatments T03 and T04 increased the pH to a value of 6.7, and, with T02, the soil reached a neutral pH (see
Figure 2a).
The initial content of organic matter (OM, see
Figure 2b) in the soil M.I. was small, at 0.6%. For T01, it increased by 0.3%, and the use of biofertilizers increased the OM significantly, especially with T03 and T04, reaching the highest value with the latter (2.73%). Thus, T03 and T04 could be classified as soils with medium OM content, while T02, like T01, rather fell into the category of soils with low OM content. A similar tendency occurred for the nitrogen content (see
Figure 2c), where the value for OM was low (14.44 kg/ha.year); however, T01 increased this value by 39.5% (20.15 kg/ha.year), and the increases with T02, T03, and T04 were even larger. This was between three and five times more than for M.I., particularly with T04 (65.6 kg/ha.year). Finally, the phosphorus content (
Figure 2d) also increased after the application of the treatments, particularly with T04 (46.85 mg/kg), where it was almost 23% higher than for T03 and T02 (~38 mg/kg), and these were in turn almost double that of T01. Even the phosphorus content in T01 was almost six times higher than in M.I.
3.3. Effects of Biofertilizers on the Biological Characteristics of the Soil: Fauna and Vegetation
The evaluation of the soil fauna, carried out before and after the application of the biofertilizers, revealed a notable increase in species richness (
Table S1), particularly with the T03 and T04 treatments, which recorded, respectively, 84 and 73 units; these values were almost three times higher than with T01 (see
Table 1). The Shannon–Wiener indices, H, reported in
Table 1, also account for the increase in biodiversity in the systems with the treatment, particularly notable with T02 and T03 (H = 2.528). It is worth mentioning that the most abundant taxonomic groups included Hymenoptera, Haplotaxis (annelids), Lepidoptera, and Diptera, with the predominance of the first two (
Table S2).
The growth and biodiversity of vegetation followed the same trend as that of the fauna described above (
Table S3), highlighting the T03 and T04 treatments, with which more than twice as many units were recorded than with T01 (
Table S4). We highlight the greater biodiversity found with T03 (H = 1.085).
3.4. Biometric Parameters: Average Height and Average Number of Leaves (nL)
Table 2 shows the average heights of growth, after 30, 60, and 120 days, of the Yaca and Mahogany plants, as well as their corresponding numbers of leaves (nL) for the different applied treatments. We can see that the Yaca plants were taller than the Mahogany plants, with a growth sequence of T04 > T03 > T02 > T01 during the first 30 days; after this, the growth rate slowed down such that the rate of T04 was similar to that of T01 (~0.13 cm/day) and somewhat lower than that of T03 (~0.15 cm/day). In the case of Mahogany, the T03 treatment was the one that most favored plant growth from the start, reaching, after 30 days, lengths with a sequence of T03 > T04 > T02 > T01. From this period onwards, the growth rates were similar to those of Yaca. It is worth mentioning that the statistical treatment of our data indicated high reliability (
p < 0.05).
The evolution, according to the treatment, of n
L, for both plants, was highly similar (see
Table 2) after 30, 60, and 120 days. The increase in n
L with T02 was equal to the increase with T01 for all periods considered. During the first 30 days, treatment T04 favored n
L, with the sequence of its increase being T04 > T03 > T02 = T01. After 30 days, the increase in n
L slowed down, and similar rates were achieved with all treatments (~0.13 leaves/day).
3.5. Chlorophyll Content in Leaves of Yaca and Mahogany
The analysis of the chlorophyll concentration in the leaves of Yaca and Mahogany (see
Table 3) revealed that the T04 treatment exhibited the highest values of total chlorophyll (0.39 μg/g) and chlorophyll A (0.27 μg/g) in Yaca, which represented, with respect to T01, an increase of 30% and ~23%, respectively. Meanwhile, in Mahogany, these increases were smaller and represented ~17% (total chlorophyll) and ~14% (chlorophyll A). It is worth mentioning that the content of total chlorophyll (0.30 μg/g) and chlorophyll A (0.22 μg/g) with T01 was similar for the leaves of both plants, and these were the lowest values of the treatments considered.
Regarding the chlorophyll B content, the highest levels corresponded to the T03 treatment for Yaca leaves (0.50 μg/g) and T04 for Mahogany leaves (0.44 μg/g). These values represent, with respect to T01, increases of 25% and 19%, respectively.
With treatment T02, for both plants, the increases in chlorophyll (total, A and B) represent, with respect to T01, almost half of the corresponding increases given with T03. On the other hand, also with T02, the increase in chlorophyll A was the same for both plants (9%), while the increases in chlorophyll B and total chlorophyll were greater in Yaca leaves (~13%) than in Mahogany leaves (~3 and ~7%).
It is important to mention that the data on the content of chlorophylls A, B and total chlorophyll in Mahogany leaves, obtained under all considered treatments, did not have statistically significant differences (p > 0.05). In contrast, except for the content of chlorophyll B, these data for Yaca were statistically well distinguishable (p < 0.05)
4. Discussion
4.1. Effects of Biofertilizers on Physical Properties
The results obtained in relation to the soil bulk density confirm that biofertilizers play a crucial role in improving the soil structure. An inverse relationship was observed between the density and water and nutrient retention capacity, in agreement with the findings of [
27]. In particular, the treatment with T04 showed the lowest density, which can be attributed to its high organic matter content and the presence of active microorganisms that facilitate the formation of stable aggregates and improve the soil porosity. This result agrees with previous studies [
28,
29] that show that the addition of biofertilizers increases biological activity and promotes the development of macropores, generating a more structured and aerated soil.
T04, being a fermented biofertilizer, presents a higher concentration of beneficial microorganisms that accelerate the decomposition of organic matter and promote the formation of a functional pore network [
30]. However, although T03 also improved the soil physical properties, its bulk density was slightly higher compared to T04, possibly due to its composition and slower mineralization rate [
31].
The results also show that the application of T04 favors the formation of macropores, which improves the water infiltration capacity and gas exchange, key factors for optimal root development [
32]. In contrast, the initial soil samples and the control treatment T01 presented lower porosity, suggesting greater compaction. According to [
33], compaction reduces root growth and nutrient availability, negatively affecting plant productivity.
Regarding the WRC, soils treated with T03 presented higher water availability compared to T01. This is consistent with the studies in [
34,
35], which argue that soils with lower porosity reach their wilting points quickly, limiting water uptake by plants at critical growth stages. The observed improvement in the WRC and porosity after the application of treatments T03 and T04 reinforces the evidence that biofertilizers can positively modify the soil physical properties, as also pointed out by previous research [
36].
From an ecological point of view, these results highlight the importance of using biofertilizers as a strategy for the regeneration of degraded soils in tropical ecosystems. The improvement in porosity and water retention not only optimizes agricultural productivity but also contributes to soil’s resilience to extreme climatic events, such as prolonged droughts [
37,
38]. This underlines the need to promote the use of biofertilizers in sustainable agricultural systems, minimizing the dependence on chemical inputs and promoting the conservation of soil biodiversity. Furthermore, it is important to note that our study was conducted under specific soil and climate conditions, so future research should include long-term trials under different agroecological conditions to validate the stability of these effects over time.
4.2. Effects of Biofertilizers on Chemical Properties
The results obtained on the pH after the application of biofertilizers coincide with the findings of [
39], which reported that an increase in soil pH is associated with higher content of nitrogen and phosphorus, elements commonly provided by biofertilizers. A pH close to neutrality is beneficial, since it improves the availability of essential nutrients such as calcium, magnesium, and potassium, in addition to favoring soil microbial activity [
39,
40]. These results are also in line with previous studies, such as that of [
37], which showed a reduction in soil acidity after the application of organic fertilizers.
The differences observed in the OM content can be attributed to the variable compositions of the biofertilizers used, which included different types of plant residues and manures [
36]. OM plays a key role in improving the soil structure, water retention capacity, and biological activity, promoting the balance of soil ecosystems [
41,
42]. The incorporation of biofertilizers, especially those with high content of fermented residues, increases microbial activity and favors the decomposition of organic matter, facilitating its integration into the soil matrix.
Regarding nitrogen availability, a variation was observed depending on the quality of the applied biofertilizer. The release of nitrogen into the soil is regulated by processes such as the mineralization of organic matter, biological fixation, and leaching losses [
43]. Previous studies [
44,
45] have shown that the application of biofertilizers enriches the soil with organic nitrogen, which, through its progressive mineralization, guarantees a long-term source of nitrogen available for plants.
The increase in phosphorus availability in treated soils is related to the increase in OM, which acts as a reservoir of this nutrient and facilitates its mineralization [
46]. Biofertilizers not only increase the total phosphorus content in the soil but also reduce losses through fixation and leaching, ensuring the greater availability of this nutrient for plants [
47]. This aspect is key, since extractable phosphorus is a fundamental indicator to evaluate the capacity of the soil to efficiently supply this nutrient for plant growth [
48].
These findings are consistent with previous research highlighting the benefits of biofertilizers in improving soil chemical properties. For example, [
49] reported significant increases in OM content and the availability of essential nutrients after the application of biofertilizers. Overall, these results underline the importance of biofertilizers as organic amendments that contribute to improving soil fertility, optimizing microbial activity, and promoting a more favorable environment for crop development.
4.3. Effects of Biofertilizers on Soil Biological Properties
The results of the Shannon–Wiener index, H, indicate that the application of biofertilizers favored a more diverse and complex soil environment, which represents a positive indicator of soil health [
50]. This finding highlights the fundamental role of soil macrofauna in the functioning of terrestrial ecosystems [
51]. In particular, treatments T03 and T04 showed the significant enrichment and diversification of these communities, evidencing the effectiveness of biofertilizers in promoting soil biodiversity.
The predominance of Hymenoptera and annelids in these treatments underlines the importance of these functional groups in the decomposition of organic matter, the regulation of biological populations, and the formation of the soil structure [
52]. In this context, earthworms play a key role as ecosystem engineers, significantly improving the soil quality through bioturbation and increasing nutrient availability, as documented in various studies [
53,
54].
The results obtained confirm that the application of T03 positively impacted the biodiversity of soil fauna, reflected in the increase in the H index. This increase suggests that T03 provides a more favorable environment for the establishment of a diverse and functionally active soil community [
55]. Previous research has also shown that vermicompost stimulates soil biological activity, strengthening the food web and promoting beneficial interactions between soil organisms [
56].
Regarding vegetation, the results suggest that biofertilizers can favor plant development, with positive implications for the recovery of degraded soils and the improvement of plant biodiversity [
57]. Although the H index did not reach the expected values, a positive trend towards greater species diversity was observed in the biofertilizer treatments [
58]. The presence of pioneer species, such as legumes and grasses, indicates that biofertilizers contribute to ecological succession, generating favorable conditions for the formation of a more complex plant community in the long term [
59].
These results emphasize the role of biofertilizers as a key tool for the restoration of degraded ecosystems and the transition towards more sustainable agricultural systems [
60]. Although the effects on vegetation in the short term were lower than expected, the application of biofertilizers has been shown to improve soil quality [
61]. By optimizing its structure and increasing nutrient availability, more favorable conditions are created for the establishment of a resilient and productive soil–plant ecosystem [
62]. Previous studies have reported a close relationship between soil macrofauna and ecosystem productivity, suggesting that the continuous use of biofertilizers can promote soil biodiversity and, consequently, increase its long-term productivity [
63].
4.4. Effects of Biofertilizers on the Biometric Properties of Yaca and Mahogany
The results show that the application of biofertilizers promoted more vigorous leaf development, although the response varies depending on the type of biofertilizer used and the soil and climate conditions. The responses observed in Yaca and Mahogany agree, in some cases, with previous findings. In this regard, it is important to mention that the literature reports diverse results. For example, [
64] reported significant increases in the height and number of leaves; however, [
65,
66] reported less pronounced effects. This diversity suggests that the optimization of the use of biofertilizers requires a detailed evaluation considering the specific factors of the context.
The incorporation of OM in biofertilizers has proven to be an effective strategy to enhance plant growth. Previous research, such as that of [
67,
68], highlights the role of organic residues in the production of high-quality biofertilizers. In particular, [
69] found that compost based on chicken manure and other organic residues significantly improved the growth of Mahogany. These findings, together with those of this study, emphasize the importance of properly selecting inputs and adjusting their application rates.
Overall, the results suggest that the application of biofertilizers not only increases the plant size but also stimulates the production of new leaves. This is relevant, since a greater number of leaves can improve the photosynthetic capacity of plants, promoting more robust and sustainable growth. The combination of an increased height and greater leaf density reinforces the potential of biofertilizers as an effective agronomic practice for crop improvement and their applicability in reforestation and tree species conservation programs.
4.5. Effects of Biofertilizers on Chlorophyll Content in Leaves of Yaco and Mahogany
Our results differ from those reported in [
70], whose authors did not find significant differences in chlorophyll content in treatments applied to
Raphanus sativus and
Lactuca sativa. This discrepancy may be related to factors such as the composition of the biofertilizers, the physiological characteristics of each species, and the environmental conditions of the experiments. According to [
71], the availability of nitrogen in the soil has a direct impact on the chlorophyll levels, although [
72] emphasized that other factors, such as the climatic conditions, also influence the nutrient assimilation and vegetative development of plants.
The analysis of photosynthetic pigments indicated that chlorophyll B was the predominant pigment in the leaves of Yaco and Mahogany. This result is consistent with those obtained in [
71], whose authors found that the proportions of chlorophyll A and B can vary significantly between species and in response to environmental factors. In addition, [
31] found variations, according to the seasonal period, in the chlorophyll concentration in
Avicennia germinans, attributing these changes to soil salinity.
The results of this study and previous research highlight the importance of considering both internal factors (plant species, physiological characteristics) and external factors (edaphoclimatic conditions and nutrient availability) in the analysis of leaf chlorophyll content. This is essential to develop more effective and sustainable agronomic strategies that maximize crop yields and favor the conservation of natural resources.
5. Conclusions
In this work, we present the results of the effects of biofertilizers, namely biol (T02), vermicompost (T03), and bokashi (T04), on the soil quality and vegetative development of Mahogany (Swietenia macrophylla) and Yaca (Artocarpus heterophyllus) cultivated in the Villa Ashaninka locality of the Peruvian Central Forest (Junin region).
Regarding the soil quality, and taking as a reference the T01 treatment (without biofertilizer), we found that the decrease in density and the increases in porosity, organic matter (OM), nitrogen, and phosphorus content were more significant with T04, while the water retention capacity (WRC) was superior with T03. Both treatments T03 and T04 slightly increased the pH of the soil. This tendency was reflected in both the fauna and the vegetative development of the soil, where the T03 and T04 treatments increased the richness of species, both in number and biodiversity (considerable Shannon–Wiener H indices) in fauna with the predominance of heminopterans and annelids.
Regarding the vegetative development of Mahogany and Yaca, the biometric parameters showed that, after the first 30 days of cultivation, the Yaca plants reached larger sizes than those of Mahogany, favored by the T04 treatment, while T03 favored the growth of Mahogany. Periods longer than 30 days (60 and 120 days) showed slower growth (approximately 0.13 cm/day) for both species, being similar with all treatments. On the other hand, the evolution of the number of leaves in Yaca was essentially the same as in Mahogany; in both, this was favored by the T04 treatment.
The chlorophyll content in the leaves of Mahogany and Yaca revealed that the highest concentrations of chlorophylls were found in the latter. The T04 treatment favored the content of chlorophylls A and total, while T03 favored the content of chlorophyll B.
Finally, we would like to highlight that the incorporation of the biofertilizer T04, and also T03, improved the quality of the soil and facilitated the vegetative development of characteristic plants, such as Yaca and Mahogany, from the Central Peruvian Jungle. However, it is important to mention that additional studies are required, such as the long-term evaluation of the nutrient dynamics of biofertilized soils in the physiological processes of plants, and it is also necessary to study the adjustment of the doses and specific formulations of these treatments to maximize their effectiveness in different agroecological contexts.
Supplementary Materials
The following supporting information can be downloaded at:
https://www.mdpi.com/article/10.3390/soilsystems9020055/s1, Table S1. Abundance of edaphic fauna found before and after fertilization; Table S2. List of fauna identified after the application of biofertilizers; Table S3. List of vegetation identified after the application of biofertilizers; Table S4. Abundance of vegetation fauna found before and after fertilization.
Author Contributions
Conceptualization, investigation, formal analysis, project administration, A.C.-C.: software, data curation, resources, J.C.-P.; validation, writing—review and editing, supervision, J.Z.D.-P.; methodology, formal analysis, visualization, writing—original draft, V.S.-A. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Data will be made available on request.
Conflicts of Interest
The authors declare no conflicts of interest.
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