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Volume 80
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10.5603/ARM.27611
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Advances in Respiratory Medicine is published by MDPI from Volume 90 Issue 4 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Via Medica.
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Article

The Use of Selected Commercial Molecular Assays for the Microbiological Diagnosis of Tuberculosis

by
Aleksandra Safianowska
*,
Renata Walkiewicz
,
Patrycja Nejman-Gryz
and
Hanna Grubek-Jaworska
Department of Internal Diseases, Pneumonology, and Allergies, Medical University of Warsaw, Banacha 1a, 02-097 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Adv. Respir. Med. 2012, 80(1), 6-12; https://doi.org/10.5603/ARM.27611
Submission received: 5 December 2011 / Revised: 21 December 2011 / Accepted: 21 December 2011 / Published: 21 December 2011

Abstract

Introduction: We performed a retrospective assessment of the AMPLICOR Mycobacterium tuberculosis (MTB) assay for the molecular diagnosis of tuberculosis based on our own determinations between 1999 and 2009 and a preliminary assessment of the Xpert MTB/RIF system, which we are currently using. Materials and methods: The study groups comprised 1875 samples (including 104 inhibited samples) and 213 samples, respectively. Results: The sensitivities of the AMPLICOR MTB and the Xpert MTB/RIF assays were 81.9% and 81.8%, respectively, and their specificities were 97.2% and 99.5%, respectively, versus culture on Loewenstein-Jensen medium. Both assays showed a considerable difference in sensitivity depending on whether the test samples were smear-positive (AFB+) or smearnegative (AFB–). The sensitivities of the AMPLICOR MTB and the Xpert MTB/RIF assays were 97.8% and 100.0%, respectively, for AFB+ samples and 58.1% and 50.0%, respectively, for AFB– samples. Conclusions: Our results confirm full usefulness of the Xpert MTB/RIF assay for routine diagnosis in the case of smearpositive clinical samples.
Keywords: amplifikacja kwasów nukleinowych; gruźlica; reakcja łańcuchowa polimerazy w czasie rzeczywistym amplifikacja kwasów nukleinowych; gruźlica; reakcja łańcuchowa polimerazy w czasie rzeczywistym

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MDPI and ACS Style

Safianowska, A.; Walkiewicz, R.; Nejman-Gryz, P.; Grubek-Jaworska, H. The Use of Selected Commercial Molecular Assays for the Microbiological Diagnosis of Tuberculosis. Adv. Respir. Med. 2012, 80, 6-12. https://doi.org/10.5603/ARM.27611

AMA Style

Safianowska A, Walkiewicz R, Nejman-Gryz P, Grubek-Jaworska H. The Use of Selected Commercial Molecular Assays for the Microbiological Diagnosis of Tuberculosis. Advances in Respiratory Medicine. 2012; 80(1):6-12. https://doi.org/10.5603/ARM.27611

Chicago/Turabian Style

Safianowska, Aleksandra, Renata Walkiewicz, Patrycja Nejman-Gryz, and Hanna Grubek-Jaworska. 2012. "The Use of Selected Commercial Molecular Assays for the Microbiological Diagnosis of Tuberculosis" Advances in Respiratory Medicine 80, no. 1: 6-12. https://doi.org/10.5603/ARM.27611

APA Style

Safianowska, A., Walkiewicz, R., Nejman-Gryz, P., & Grubek-Jaworska, H. (2012). The Use of Selected Commercial Molecular Assays for the Microbiological Diagnosis of Tuberculosis. Advances in Respiratory Medicine, 80(1), 6-12. https://doi.org/10.5603/ARM.27611

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