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Open AccessFeature PaperArticle

Adsorption of Hyperbranched Arabinogalactan-Proteins from Plant Exudate at the Solid–Liquid Interface

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UR1268 Biopolymères Interactions Assemblages, INRA, F-44300 Nantes, France
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UMR IATE, UM-INRA-CIRAD-Montpellier Supagro, 2 Place Viala, F-34060 Montpellier CEDEX, France
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BIBS, INRA, F-44300 Nantes, France
*
Author to whom correspondence should be addressed.
Colloids Interfaces 2019, 3(2), 49; https://doi.org/10.3390/colloids3020049
Received: 24 April 2019 / Revised: 14 May 2019 / Accepted: 15 May 2019 / Published: 17 May 2019
Adsorption of hyperbranched arabinogalactan-proteins (AGPs) from two plant exudates, A. senegal and A. seyal, was thoroughly studied at the solid–liquid interface using quartz crystal microbalance with dissipation monitoring (QCM-D), surface plasmon resonance (SPR), and atomic force microscopy (AFM). Isotherms of the adsorption reveal that 3.3 fold more AGPs from A. seyal (500 ppm) are needed to cover the gold surface compared to A. senegal (150 ppm). The pH and salt concentration of the environment greatly affected the adsorption behavior of both gums, with the surface density ranging from 0.92 to 3.83 mg m−2 using SPR (i.e., “dry” mass) and from 1.16 to 19.07 mg m−2 using QCM-D (wet mass). Surprisingly, the mass adsorbed was the highest in conditions of strong electrostatic repulsions between the gold substrate and AGPs, i.e., pH 7.0, highlighting the contribution of other interactions involved in the adsorption process. Structural changes of AGPs induced by pH would result in swelling of the polysaccharide blocks and conformational changes of the polypeptide backbone, therefore increasing the protein accessibility and hydrophobic interactions and/or hydrogen bonds with the gold substrate. View Full-Text
Keywords: Acacia gum; gold; adsorption; arabinogalactan-protein; flexibility; quartz crystal microbalance; surface plasmon resonance; atomic force microscopy Acacia gum; gold; adsorption; arabinogalactan-protein; flexibility; quartz crystal microbalance; surface plasmon resonance; atomic force microscopy
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Davantès, A.; Nigen, M.; Sanchez, C.; d’Orlando, A.; Renard, D. Adsorption of Hyperbranched Arabinogalactan-Proteins from Plant Exudate at the Solid–Liquid Interface. Colloids Interfaces 2019, 3, 49.

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