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Open AccessArticle

Culture Medium Optimization for Production of Rhamnolipids by Burkholderia glumae

INRS-Institut Armand-Frappier, Laval, QC H7V 1B7, Canada
Author to whom correspondence should be addressed.
Colloids Interfaces 2018, 2(4), 49;
Received: 6 September 2018 / Revised: 10 October 2018 / Accepted: 15 October 2018 / Published: 18 October 2018
(This article belongs to the Special Issue Biosurfactants: Trends and Applications)
Burkholderia glumae is a biosafety level 1 bacterium capable of producing rhamnolipid biosurfactant with longer 3-hydroxy fatty acid chains moieties than those produced by the prototypal producer, the opportunistic pathogen Pseudomonas aeruginosa. Although the capacity of production of rhamnolipid, and the parameters affecting this production, are well established for P. aeruginosa, little is known about the factors that may affect their production in B. glumae. Hence, to evaluate and enhance the production of rhamnolipids in B. glumae, following the selection of best carbon and nitrogen sources, a two-level fractional factorial design experiment was performed to identify the limiting factors significantly affecting the production of rhamnolipids in this bacterial species. Effects of six inorganic nutrients and two physical parameters were studied, and mannitol, urea, CaCl2, and potassium phosphate buffer were selected for further optimization by applying a response surface methodology (RSM). Under the identified optimized conditions, a rhamnolipid production of 1.66 g/L was obtained, about five times higher than that of the initial non-optimized conditions. This represents a key step in the development of large-scale production processes. View Full-Text
Keywords: biosurfactant; nonpathogenic bacterium; response surface methodology (RSM) biosurfactant; nonpathogenic bacterium; response surface methodology (RSM)
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Nickzad, A.; Guertin, C.; Déziel, E. Culture Medium Optimization for Production of Rhamnolipids by Burkholderia glumae. Colloids Interfaces 2018, 2, 49.

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