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3 March 2020

Ensiling Unsalable Vegetables with Crop Sorghum to Produce High Quality Feed †

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1
School of Agriculture and Food Sciences, Faculty of Science, The University of Queensland, Gatton, QLD 4343, Australia
2
School of Life and Environment Sciences, Faculty of Science, The University of Sydney, Camperdown, NSW 2006, Australia
3
Department of Agricultural, Forestry and Food Science, University of Turin, 10124 Torino, Italy
4
Lacombe Research and Development Centre, Agriculture and Agri-Food Canada, Lacombe, AB T4L 1W1, Canada
This article belongs to the Proceedings The Third International Tropical Agriculture Conference (TROPAG 2019)

Abstract

Vegetables regarded as unsalable at processing often undergo disposal into landfill, threatening food security and increasing emissions through decomposition. Ensiling vegetables with forage crops is a suggested method of waste reduction that could also double as a livestock feed. Carrot or pumpkin, ensiled at 0%, 20% or 40% DM with crop sorghum, and with or without a second-generation silage inoculant were assessed for nutritive composition, organic acid profiles, aerobic stability and in vitro rumen fermentation characteristics. Silage was sampled after 70-days ensiling for nutrient composition, 14-day aerobic stability, organic acid profiles and microbial diversity. Sorghum ensiled with carrot or pumpkin at 20% or 40% DM increased crude fat (P ≤ 0.01) and decreased (P ≤ 0.01) silage surface temperature upon aerobic exposure compared to the control. Bacterial communities analyzed through 16S rRNA gene sequencing linearly increased (P ≤ 0.01) in diversity as vegetable proportion increased in the silage; dominated by Lactobacillus species. Upon assessment in vitro, there was an increase (P ≤ 0.04) in in vitro digestibility and some CH4 parameters (% CH4, and mg CH4/g DM), with no effect (P ≥ 0.17) on remaining CH4 parameters (mL CH4/g DM, mg CH4/g DMD), gas production or pH. However, increasing vegetable proportion decreased acetic and increased propionic acid concentrations respectively, decreasing A:P ratio and total VFA as a result (P ≤ 0.01). Results from this study indicate including carrot or pumpkin at 20% or 40% DM in a sorghum silage can produce a highly digestible, microbially diverse and energy-rich livestock feed whilst acting as a method of waste diversion of considerable environmental benefit.

Supplementary Materials

Figures, tables and supplementary materials are available online at https://www.frontiersin.org/articles/10.3389/fmicb.2019.02599/full#S10.

Author Contributions

A.V.C. and S.J.M. designed the study. A.V.C., S.J.M., D.L.F., K.H., E.C., Y.H. and D.H. acquired data, read and critically revised drafts for intellectual contents, and approved the final manuscript. E.C., D.L.F., K.H., Y.H. and A.V.C. conducted laboratory analysis. D.H. conducted bioinformatics. A.V.C. and D.H. ran statistical analysis. D.L.F., K.H., D.H., S.J.M. and A.V.C. wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Acknowledgments

The authors would like to thank Katherine Gzyl and Arun Kommadath for assistance in processing the 16S rRNA gene and ITS1 sequence data. We would also like to thank Kalfresh (Kalbar, QLD, Australia) for providing the unsalable vegetables used in this study.

Conflicts of Interest

The authors declare no conflict of interest.
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