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Open AccessProceedings

Continuous Live-Cell Culture Monitoring by Compact Lensless LED Microscopes

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Institute of Semiconductor Technology (IHT), TU Braunschweig, 38106 Braunschweig, Germany
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Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, 38106 Braunschweig, Germany
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Research Center for Electronics and Telecommunication, Indonesian Institute of Sciences (LIPI), 40135 Bandung, Indonesia
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Faculty of Information Technology, Universitas Tarumanagara, 11440 Jakarta, Indonesia
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Institute of Pharmacology, Toxicology and Clinical Pharmacy (IPT), TU Braunschweig, 38106 Braunschweig, Germany
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Institute of Microtechnology (IMT), TU Braunschweig, 38124 Braunschweig, Germany
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Institute for Biochemistry, Biotechnology and Bioinformatics, TU Braunschweig, 38106 Braunschweig, Germany
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MIND-IN2UB, Department of Electronic and Biomedical Engineering, University of Barcelona, 08028 Barcelona, Spain
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Authors to whom correspondence should be addressed.
Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018.
Proceedings 2018, 2(13), 877; https://doi.org/10.3390/proceedings2130877
Published: 5 December 2018
(This article belongs to the Proceedings of EUROSENSORS 2018)
A compact lensless microscope comprising a custom-made LED engine and a CMOS imaging sensor has been developed for live-cell culture imaging inside a cell incubator environment. The imaging technique is based on digital inline-holographic microscopy, while the image reconstruction is carried out by angular spectrum approach with a custom written software. The system was tested with various biological samples including immortalized mouse astrocyte cells inside a petri dish. Besides the imaging possibility, the capability of automated cell counting and tracking could be demonstrated. By using image sensors capable of video frame rate, time series of cell movement can be captured.
Keywords: lensless holographic microscopy; LED; CMOS image sensor; cell culture; cell imaging; cell counting lensless holographic microscopy; LED; CMOS image sensor; cell culture; cell imaging; cell counting
MDPI and ACS Style

Scholz, G.; Mariana, S.; Syamsu, I.; Dharmawan, A.B.; Schulze, T.; Mattern, K.; Hörmann, P.; Hartmann, J.; Dietzel, A.; Rustenbeck, I.; Hiller, K.; Prades, J.D.; Waag, A.; Wasisto, H.S. Continuous Live-Cell Culture Monitoring by Compact Lensless LED Microscopes. Proceedings 2018, 2, 877.

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