Effect of Bioavailable Whey Peptides on C2C12 Muscle Cells †
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Lines
2.3. Alamar Blue Assay
2.4. Cellular Antioxidant Activity
2.5. Statistical Analysis
3. Results and Discussion
3.1. Alamar Blue
3.2. Protection against Free Radicals
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Naseeb, M.A.; Volpe, S.L. Protein and exercise in the prevention of sarcopenia and aging. Nutr. Res. 2017, 40, 1–20. [Google Scholar] [CrossRef] [PubMed]
- Corrochano, A.R.; Arranz, E.; De Noni, I.; Stuknyte, M.; Ferraretto, A.; Kelly, P.M.; Buckin, V.; Giblin, L. Intestinal health benefits of bovine whey proteins after simulated gastrointestinal digestion. J. Funct. Foods 2018, 49, 526–535. [Google Scholar] [CrossRef]
- Valerio, A.; D’Antona, G.; Nisoli, E. Branched-chain amino acids, mitochondrial biogenesis, and healthspan: An evolutionary perspective. Aging-Us 2011, 3, 464–478. [Google Scholar] [CrossRef] [PubMed]
- Davies, R.W.; Bass, J.J.; Carson, B.P.; Norton, C.; Kozior, M.; Brook, M.S.; Wilkinson, D.J.; Atherton, P.J.; Smith, K.; Jakeman, P.M. The Effect of whey protein supplementation on the recovery of contractile function following resistance training. Med. Sci. Sport Exer. 2018, 50, 839. [Google Scholar] [CrossRef]
- Lafarga, T.; Aluko, R.E.; Rai, D.K.; O’Connor, P.; Hayes, M. Identification of bioactive peptides from a papain hydrolysate of bovine serum albumin and assessment of an antihypertensive effect in spontaneously hypertensive rats. Food Res. Int. 2016, 81, 91–99. [Google Scholar] [CrossRef]
- Corrochano, A.R.; Buckin, V.; Kelly, P.M.; Giblin, L. Invited review: Whey proteins as antioxidants and promoters of cellular antioxidant pathways. J. Dairy Sci. 2018, 101, 4747–4761. [Google Scholar] [CrossRef] [PubMed]
- Basilicata, M.G.; Pepe, G.; Adesso, S.; Ostacolo, C.; Sala, M.; Sommella, E.; Scala, M.C.; Messore, A.; Autore, G.; Marzocco, S.; et al. Antioxidant properties of buffalo-milk dairy products: A beta-Lg peptide released after gastrointestinal digestion of buffalo ricotta cheese reduces oxidative stress in intestinal epithelial cells. Int. J. Mol. Sci. 2018, 19. [Google Scholar] [CrossRef] [PubMed]
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Knowles, S.; Gilmartin, S.; Arranz, E.; O’Brien, N.; Giblin, L. Effect of Bioavailable Whey Peptides on C2C12 Muscle Cells. Proceedings 2019, 11, 35. https://doi.org/10.3390/proceedings2019011035
Knowles S, Gilmartin S, Arranz E, O’Brien N, Giblin L. Effect of Bioavailable Whey Peptides on C2C12 Muscle Cells. Proceedings. 2019; 11(1):35. https://doi.org/10.3390/proceedings2019011035
Chicago/Turabian StyleKnowles, Stephen, Sarah Gilmartin, Elena Arranz, Nora O’Brien, and Linda Giblin. 2019. "Effect of Bioavailable Whey Peptides on C2C12 Muscle Cells" Proceedings 11, no. 1: 35. https://doi.org/10.3390/proceedings2019011035
APA StyleKnowles, S., Gilmartin, S., Arranz, E., O’Brien, N., & Giblin, L. (2019). Effect of Bioavailable Whey Peptides on C2C12 Muscle Cells. Proceedings, 11(1), 35. https://doi.org/10.3390/proceedings2019011035