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Chlorogenic Acid as a Model Compound for Optimization of an In Vitro Gut Microbiome-Metabolism Model

1
Natural Products and Food-Research & Analysis (NatuRA), University of Antwerp, 2610 Wilrijk, Belgium
2
Toxicological Centre, University of Antwerp, 2610 Wilrijk, Belgium
3
Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, University of Antwerp, 2610 Wilrijk, Belgium
4
Department of Critical Care Medicine, Antwerp University Hospital, Clinical Pharmacotherapy and Toxicology, University of Antwerp, 2650 Edegem, Belgium
*
Author to whom correspondence should be addressed.
Presented at Natural Products and the Hallmarks of Chronic Diseases—COST Action 16112, Luxemburg, 25–27 March 2019.
Proceedings 2019, 11(1), 31; https://doi.org/10.3390/proceedings2019011031
Published: 19 April 2019
PDF [207 KB, uploaded 19 April 2019]

Abstract

It has been believed that the metabolism of xenobiotics occurred mainly by the cytochrome P450 enzyme system in the liver. However, recent data clearly suggest a significant role for the gut microbiota in the metabolism of xenobiotic compounds. This microbiotic biotransformation could lead to differences on activation, inactivation and possible toxicity of these compounds. In vitro models are generally used to study the colonic biotransformation as they allow easy dynamic and multiple sampling over time. However, to ensure this accurately mimics communities in vivo, the pre-analytical phase requires optimization. Chlorogenic acid, a polyphenolic compound abundantly present in the human diet, was used as a model compound to optimize a ready-to-use gut microbiome biotransformation platform. Samples of the in vitro gastrointestinal dialysis-model with colon stage were analyzed by liquid chromatography coupled to high resolution time-of-flight mass spectrometry. Complementary screening approaches were also employed to identify the biotransformation products.
Keywords: gut microbiome; in vitro gastrointestinal dialysis model; 16S rDNA sequencing; liquid chromatography-mass spectrometry gut microbiome; in vitro gastrointestinal dialysis model; 16S rDNA sequencing; liquid chromatography-mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Mortelé, O.; Iturrospe, E.; Breynaert, A.; Lammens, C.; Britto, X.B.; Malhotra-Kumar, S.; Jorens, P.; Pieters, L.; Nuijs, A.L.N.; Hermans, N. Chlorogenic Acid as a Model Compound for Optimization of an In Vitro Gut Microbiome-Metabolism Model. Proceedings 2019, 11, 31.

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