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Proceedings 2019, 11(1), 20; https://doi.org/10.3390/proceedings2019011020

DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method

1
Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland
2
Institute for Biological Research “Siniša Stanković”, University of Belgrade, 11060 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Presented at Natural Products and the Hallmarks of Chronic Diseases—COST Action 16112, Luxemburg 25–27 March 2019.
Published: 17 April 2019
PDF [245 KB, uploaded 17 April 2019]

Abstract

The impact of catechins on the expression profile of redox-related genes in HT29 cell line has been studied recently by our group using Oxidative Stress RT2 Profiler PCR Array. Within the examined panel of 84 genes, the down-regulation of SRXN1 gene was unique among other up-regulated genes. We hypothesized that the observed down-regulation resulted from DNA methylation and have exploited this observation to choose the proper strategy to monitor the changes in DNA methylation patterns incurred by dietary antioxidants. The current study verified two PCR-based approaches.
Keywords: catechins; DNA methylation; methylation-specific PCR; methylation-sensitive high resolution melting; redox homeostasis; epigenetics catechins; DNA methylation; methylation-specific PCR; methylation-sensitive high resolution melting; redox homeostasis; epigenetics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Jakubek, P.; Rajić, J.; Baranowska, M.; Vidaković, M.; Bartoszek, A.; Namieśnik, J. DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method. Proceedings 2019, 11, 20.

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